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FAM46D (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FAM46D
Gene Name:  family with sequence similarity 46, member D
Sequence             
Original Clone ID: ccsbBroad304_14423
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435012

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1167nts         Open reading frame : 1 to 1167
Reference Sequence Alignment

ATGTCTGAAATCAGATTCACCAATCTCGCTTGGGATCAAGTTATAACACTGGATCAAGTG
TTAGATGAAGTAATTCCAATTCATGGAAAGGGGAATTTCCCCACAATGGAGGTAAAACCA
AAAGACATCATTCATGTTGTGAAAGATCAACTCATAGGGCAAGGAATTATTGTTAAAGAT
GCCAGATTGAATGGTTCCGTAGCAAGTTACATACTTGCAAGCCACAATGGAATCAGCTAT
AAGGATCTGGACGTTATTTTTGGTGTTGAGCTTCCAGGTAACGAAGAATTTCAGGTTGTT
AAAGATGCAGTTCTAGACTGTCTACTTGACTTTTTACCAAAAGATGTAAAGAAGGAAAAG
CTCTCCCCAGATATCATGAAAGACGCTTACGTACAGAAATTGGTCAAGGTTTGCAATGGG
CATGATTGTTGGAGTCTTATCTCCCTTTCAAATAACACTGGGAAGAATTTAGAACTAAAA
TTTGTGAGTTCACTCAGACGGCAGTTTGAATTTAGTGTAGATTCCTTTCAAATTGTTTTG
GATCCCATGTTAGACTTCTACAGTGACAAAAATGCCAAGCTAACCAAAGAATCCTATCCT
GTTGTGGTAGCTGAAAGCATGTATGGAGACTTCCAGGAAGCAATGACACATTTGCAACAC
AAGCTCATATGTACCAGGAAACCTGAAGAGATTAGAGGTGGTGGCCTTCTGAAGTACTGC
AGCTTGCTGGTTCATGGCTTCAAGCCAGCCTGTATGTCAGAAATCAAAAACCTAGAACGT
TATATGTGCTCTAGATTCTTTATTGATTTTCCTCATATAGAAGAACAGCAAAAGAAAATT
GAATCATACCTCCACAACCATTTCATAGGTGAAGGAATGACCAAGTATGACTACCTTATG
ACCTTGCATGGAGTTGTGAATGAAAGCACTGTTTGCCTCATGAGTTATGAAAGAAGACAG
ATTCTCCACCTGATCACCATGATGGCTTTGAAAGTACTTGGAGAACTAAATATTCTACCC
AATACACAAAAGGTAACTTGCTTTTATCAGCCTGCTCCGTACTTTGCAGCTGAGGCAAGG
TACCCTATTTATGTAATACCTGAGCCACCCCCCGTTAGCTTCCAGCCATACCACCCACTG
CACCTTCGTGTATCAAATGGTTGAGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; 2 non-synonomous errors;
 early stop codon; insertion or deletion in coding sequence
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FAM46D
Symbol Nomenclature : FAM46D
SYNONYM : CT1.26; CT112
Designation : cancer/testis antigen 112|protein FAM46D
Full Nomenclature : family with sequence similarity 46, member D
GENEID : 169966
HGNC : 28399
Vega : OTTHUMG00000021902
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00435012        ATGTCTGAAATCAGATTCACCAATCTCGCTTGGGATCAAGTTATAACACTGGATCAAGTG
NM_001170574.1      ATGTCTGAAATCAGATTCACCAATCTCACTTGGGATCAAGTTATAACACTGGATCAAGTG
NM_152630.4         ATGTCTGAAATCAGATTCACCAATCTCACTTGGGATCAAGTTATAACACTGGATCAAGTG
                    ***************************.********************************

HsCD00435012        TTAGATGAAGTAATTCCAATTCATGGAAAGGGGAATTTCCCCACAATGGAGGTAAAACCA
NM_001170574.1      TTAGATGAAGTAATTCCAATTCATGGAAAGGGGAATTTCCCCACAATGGAGGTAAAACCA
NM_152630.4         TTAGATGAAGTAATTCCAATTCATGGAAAGGGGAATTTCCCCACAATGGAGGTAAAACCA
                    ************************************************************

HsCD00435012        AAAGACATCATTCATGTTGTGAAAGATCAACTCATAGGGCAAGGAATTATTGTTAAAGAT
NM_001170574.1      AAAGACATCATTCATGTTGTGAAAGATCAACTCATAGGGCAAGGAATTATTGTTAAAGAT
NM_152630.4         AAAGACATCATTCATGTTGTGAAAGATCAACTCATAGGGCAAGGAATTATTGTTAAAGAT
                    ************************************************************

HsCD00435012        GCCAGATTGAATGGTTCCGTAGCAAGTTACATACTTGCAAGCCACAATGGAATCAGCTAT
NM_001170574.1      GCCAGATTGAATGGTTCCGTAGCAAGTTACATACTTGCAAGCCACAATGGAATCAGCTAT
NM_152630.4         GCCAGATTGAATGGTTCCGTAGCAAGTTACATACTTGCAAGCCACAATGGAATCAGCTAT
                    ************************************************************

HsCD00435012        AAGGATCTGGACGTTATTTTTGGTGTTGAGCTTCCAGGTAACGAAGAATTTCAGGTTGTT
NM_001170574.1      AAGGATCTGGACGTTATTTTTGGTGTTGAGCTTCCAGGTAACGAAGAATTTCAGGTTGTT
NM_152630.4         AAGGATCTGGACGTTATTTTTGGTGTTGAGCTTCCAGGTAACGAAGAATTTCAGGTTGTT
                    ************************************************************

HsCD00435012        AAAGATGCAGTTCTAGACTGTCTACTTGACTTTTTACCAAAAGATGTAAAGAAGGAAAAG
NM_001170574.1      AAAGATGCAGTTCTAGACTGTCTACTTGACTTTTTACCAAAAGATGTAAAGAAGGAAAAG
NM_152630.4         AAAGATGCAGTTCTAGACTGTCTACTTGACTTTTTACCAAAAGATGTAAAGAAGGAAAAG
                    ************************************************************

HsCD00435012        CTCTCCCCAGATATCATGAAAGACGCTTACGTACAGAAATTGGTCAAGGTTTGCAATGGG
NM_001170574.1      CTCTCCCCAGATATCATGAAAGACGCTTACGTACAGAAATTGGTCAAGGTTTGCAATGGG
NM_152630.4         CTCTCCCCAGATATCATGAAAGACGCTTACGTACAGAAATTGGTCAAGGTTTGCAATGGG
                    ************************************************************

HsCD00435012        CATGATTGTTGGAGTCTTATCTCCCTTTCAAATAACACTGGGAAGAATTTAGAACTAAAA
NM_001170574.1      CATGATTGTTGGAGTCTTATCTCCCTTTCAAATAACACTGGGAAGAATTTAGAACTAAAA
NM_152630.4         CATGATTGTTGGAGTCTTATCTCCCTTTCAAATAACACTGGGAAGAATTTAGAACTAAAA
                    ************************************************************

HsCD00435012        TTTGTGAGTTCACTCAGACGGCAGTTTGAATTTAGTGTAGATTCCTTTCAAATTGTTTTG
NM_001170574.1      TTTGTGAGTTCACTCAGACGGCAGTTTGAATTTAGTGTAGATTCCTTTCAAATTGTTTTG
NM_152630.4         TTTGTGAGTTCACTCAGACGGCAGTTTGAATTTAGTGTAGATTCCTTTCAAATTGTTTTG
                    ************************************************************

HsCD00435012        GATCCCATGTTAGACTTCTACAGTGACAAAAATGCCAAGCTAACCAAAGAATCCTATCCT
NM_001170574.1      GATCCCATGTTAGACTTCTACAGTGACAAAAATGCCAAGCTAACCAAAGAATCCTATCCT
NM_152630.4         GATCCCATGTTAGACTTCTACAGTGACAAAAATGCCAAGCTAACCAAAGAATCCTATCCT
                    ************************************************************

HsCD00435012        GTTGTGGTAGCTGAAAGCATGTATGGAGACTTCCAGGAAGCAATGACACATTTGCAACAC
NM_001170574.1      GTTGTGGTAGCTGAAAGCATGTATGGAGACTTCCAGGAAGCAATGACACATTTGCAACAC
NM_152630.4         GTTGTGGTAGCTGAAAGCATGTATGGAGACTTCCAGGAAGCAATGACACATTTGCAACAC
                    ************************************************************

HsCD00435012        AAGCTCATATGTACCAGGAAACCTGAAGAGATTAGAGGTGGTGGCCTTCTGAAGTACTGC
NM_001170574.1      AAGCTCATATGTACCAGGAAACCTGAAGAGATTAGAGGTGGTGGCCTTCTGAAGTACTGC
NM_152630.4         AAGCTCATATGTACCAGGAAACCTGAAGAGATTAGAGGTGGTGGCCTTCTGAAGTACTGC
                    ************************************************************

HsCD00435012        AGCTTGCTGGTTCATGGCTTCAAGCCAGCCTGTATGTCAGAAATCAAAAACCTAGAACGT
NM_001170574.1      AGCTTGCTGGTTCATGGCTTCAAGCCAGCCTGTATGTCAGAAATCAAAAACCTAGAACGT
NM_152630.4         AGCTTGCTGGTTCATGGCTTCAAGCCAGCCTGTATGTCAGAAATCAAAAACCTAGAACGT
                    ************************************************************

HsCD00435012        TATATGTGCTCTAGATTCTTTATTGATTTTCCTCATATAGAAGAACAGCAAAAGAAAATT
NM_001170574.1      TATATGTGCTCTAGATTCTTTATTGATTTTCCTCATATAGAAGAACAGCAAAAGAAAATT
NM_152630.4         TATATGTGCTCTAGATTCTTTATTGATTTTCCTCATATAGAAGAACAGCAAAAGAAAATT
                    ************************************************************

HsCD00435012        GAATCATACCTCCACAACCATTTCATAGGTGAAGGAATGACCAAGTATGACTACCTTATG
NM_001170574.1      GAATCATACCTCCACAACCATTTCATAGGTGAAGGAATGACCAAGTATGACTACCTTATG
NM_152630.4         GAATCATACCTCCACAACCATTTCATAGGTGAAGGAATGACCAAGTATGACTACCTTATG
                    ************************************************************

HsCD00435012        ACCTTGCATGGAGTTGTGAATGAAAGCACTGTTTGCCTCATGAGTTATGAAAGAAGACAG
NM_001170574.1      ACCTTGCATGGAGTTGTGAATGAAAGCACTGTTTGCCTCATGAGTTATGAAAGAAGACAG
NM_152630.4         ACCTTGCATGGAGTTGTGAATGAAAGCACTGTTTGCCTCATGAGTTATGAAAGAAGACAG
                    ************************************************************

HsCD00435012        ATTCTCCACCTGATCACCATGATGGCTTTGAAAGTACTTGGAGAACTAAATATTCTACCC
NM_001170574.1      ATTCTCCACCTGATCACCATGATGGCTTTGAAAGTACTTGGAGAACTAAATATTCTACCC
NM_152630.4         ATTCTCCACCTGATCACCATGATGGCTTTGAAAGTACTTGGAGAACTAAATATTCTACCC
                    ************************************************************

HsCD00435012        AATACACAAAAGGTAACTTGCTTTTATCAGCCTGCTCCGTACTTTGCAGCTGAGGCAAGG
NM_001170574.1      AATACACAAAAGGTAACTTGCTTTTATCAGCCTGCTCCGTACTTTGCAGCTGAGGCAAGG
NM_152630.4         AATACACAAAAGGTAACTTGCTTTTATCAGCCTGCTCCGTACTTTGCAGCTGAGGCAAGG
                    ************************************************************

HsCD00435012        TACCCTATTTATGTAATACCTGAGCCACCCCCCGTTAGCTTCCAGCCATACCACCCACTG
NM_001170574.1      TACCCTATTTATGTAATACCTGAGCCACCCCCCGTTAGCTTCCAGCCATACCACCCACTG
NM_152630.4         TACCCTATTTATGTAATACCTGAGCCACCCCCCGTTAGCTTCCAGCCATACCACCCACTG
                    ************************************************************

HsCD00435012        CACCTTCGTGTATCAAATGGTTGAGT----
NM_001170574.1      CACTTTCGTGGATCAAATGGTATGAGTTAA
NM_152630.4         CACTTTCGTGGATCAAATGGTATGAGTTAA
                    *** ****** **********: ..     

Protein

SMART domain : DUF1693 : Domain of unknown function (DUF1693)
HPRD : 06643

Clone

Original Clone ID : ccsbBroad304_14423

Organism


TAX_ID : 9606
Species Specific ID: 169966

Genome

Chromosome : X
Map Location : Xq21.1
Ensembl : ENSG00000174016

Reagents

Labome : FAM46D-antibody