DNASU Plasmid Repository • 480.965.5697 | Email

ITGB8 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ITGB8
Gene Name:  integrin, beta 8
Sequence             
Original Clone ID: ccsbBroad304_10927
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00435192

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1318nts        
Open reading frame : 1 to 1318
Reference Sequence Alignment

ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter CMV CMV promoter 14 566
promoter T7 T7 promoter 3690 3672
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker BlastR Blasticidin resistance gene 8753 9148
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ITGB8
Symbol Nomenclature : ITGB8
Designation : integrin beta-8
Full Nomenclature : integrin, beta 8
GENEID : 3696
HGNC : 6163
MIM : 604160
Vega : OTTHUMG00000023594
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00435192      ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
NM_002214.2       ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
                  ************************************************************

HsCD00435192      CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
NM_002214.2       CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
                  ************************************************************

HsCD00435192      CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
NM_002214.2       CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
                  ************************************************************

HsCD00435192      CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
NM_002214.2       CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
                  ************************************************************

HsCD00435192      GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
NM_002214.2       GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
                  ************************************************************

HsCD00435192      TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
NM_002214.2       TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
                  ************************************************************

HsCD00435192      GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
NM_002214.2       GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
                  ************************************************************

HsCD00435192      CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
NM_002214.2       CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
                  ************************************************************

HsCD00435192      AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
NM_002214.2       AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
                  ************************************************************

HsCD00435192      TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
NM_002214.2       TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
                  ************************************************************

HsCD00435192      AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
NM_002214.2       AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
                  ************************************************************

HsCD00435192      CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
NM_002214.2       CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
                  ************************************************************

HsCD00435192      GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
NM_002214.2       GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
                  ************************************************************

HsCD00435192      CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
NM_002214.2       CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
                  ************************************************************

HsCD00435192      CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
NM_002214.2       CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
                  ************************************************************

HsCD00435192      GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
NM_002214.2       GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
                  ************************************************************

HsCD00435192      GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
NM_002214.2       GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
                  ************************************************************

HsCD00435192      TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
NM_002214.2       TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
                  ************************************************************

HsCD00435192      ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
NM_002214.2       ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
                  ************************************************************

HsCD00435192      TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
NM_002214.2       TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
                  ************************************************************

HsCD00435192      TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
NM_002214.2       TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
                  ************************************************************

HsCD00435192      AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAAC---
NM_002214.2       AACGTGACGAGCAATGATGAAGTTCTTTTCAATGTAACAGTTACAATGAAAAAATGTGAT
                  ***********************:  *   :.** *: : * .*::* ****:*:.    

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GTCACAGGAGGAAAAAACTATGCAATAATCAAACCTATTGGTTTTAATGAAACCGCTAAA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       ATTCATATACACAGAAACTGCAGCTGTCAGTGTGAGGACAACAGAGGACCTAAAGGAAAG
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       TGTGTAGATGAAACTTTTCTAGATTCCAAGTGTTTCCAGTGTGATGAGAATAAATGTCAT
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       TTTGATGAAGATCAGTTTTCTTCTGAGAGTTGCAAGTCACACAAGGATCAGCCTGTTTGC
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       AGTGGTCGAGGAGTTTGTGTTTGTGGGAAATGTTCATGTCACAAAATTAAGCTTGGAAAA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GTGTATGGAAAATACTGTGAAAAGGATGACTTTTCTTGTCCATATCACCATGGAAATCTG
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       TGTGCTGGGCATGGAGAGTGTGAAGCAGGCAGATGCCAATGCTTCAGTGGCTGGGAAGGT
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GATCGATGCCAGTGCCCTTCAGCAGCAGCCCAGCACTGTGTCAATTCAAAGGGCCAAGTG
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       TGCAGTGGAAGAGGCACGTGTGTGTGTGGAAGGTGTGAGTGCACCGATCCCAGGAGCATC
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GGCCGCTTCTGTGAACACTGCCCCACCTGTTATACAGCCTGCAAGGAAAACTGGAATTGT
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       ATGCAATGCCTTCACCCTCACAATTTGTCTCAGGCTATACTTGATCAGTGCAAAACCTCA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       TGTGCTCTCATGGAACAACAGCATTATGTCGACCAAACTTCAGAATGTTTCTCCAGCCCA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       AGCTACTTGAGAATATTTTTCATCATTTTCATAGTTACATTCTTGATTGGGTTGCTTAAA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GTCCTGATCATTAGACAGGTGATACTACAATGGAATAGTAATAAAATTAAGTCCTCATCA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GATTACAGAGTGTCAGCCTCAAAAAAGGATAAGTTGATTCTGCAAAGTGTTTGCACAAGA
                                                                              

HsCD00435192      ------------------------------------------------------------
NM_002214.2       GCAGTCACCTACCGACGTGAGAAGCCTGAAGAAATAAAAATGGATATCAGCAAATTAAAT
                                                                              

HsCD00435192      ------------------------------
NM_002214.2       GCTCATGAAACTTTCAGGTGCAACTTCTAA
                                                

Pathways

NCI : Beta5 beta6 beta7 and beta8 integrin cell surface interactions
NCI : Integrin family cell surface interactions
Panther : Integrin signalling pathway
Reactome : Elastic fibre formation
Reactome : Extracellular matrix organization
Reactome : Integrin cell surface interactions
Reactome : Molecules associated with elastic fibres
WikiPathway : Focal Adhesion
WikiPathway : Integrin-mediated cell adhesion

Clone

Original Clone ID : ccsbBroad304_10927

Organism


TAX_ID : 9606
Species Specific ID: 3696

Genome

Chromosome : 7
Map Location : 7p21.1
Ensembl : ENSG00000105855

Reagents

Labome : ITGB8-antibody