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RP11-444E17.2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TCTE1
Gene Name:  t-complex-associated-testis-expressed 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177608.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043528

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1506nts         Open reading frame : 1 to 1506
Reference Sequence Alignment

ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCTACAGCTCTCAAAGCCT
TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACGTCCCAGGGCCAATATCCGTCGG
ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
TTCCTCTTCACCTACCGCGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
GACCGTGGTGCACGAGGCGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
GCCCTGAATCCCAGCCACTTCATGTCAACCATCACTGCCAATGGCCCTGAGAACTCTGTG
GGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1634
Start on reference sequence 129
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCTE1
Symbol Nomenclature : TCTE1
SYNONYM : D6S46
Designation : T-complex-associated testis-expressed protein 1|tcte-1
Full Nomenclature : t-complex-associated-testis-expressed 1
GENEID : 202500
Locus Tag : RP11-444E17.2
HGNC : 11693
MIM : 186975
Target GenBank: BC035022

 

Reference Sequence Alignment

  

HsCD00043528        ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
NM_182539.3         ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
XM_005248872.1      ATGCAGGATACCGTAACGACATCAGCATTGTTGGACCCCAGCCACTCCTCAGTCTCCACC
                    ************************************************************

HsCD00043528        CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCTACAGCTCTCAAAGCCT
NM_182539.3         CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCCACAGCTCTCAAAGCCT
XM_005248872.1      CAGGACAATTCCTCCACTGGAGGACACACTTCAAGCACAAGCCCACAGCTCTCAAAGCCT
                    ******************************************* ****************

HsCD00043528        TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACGTCCCAGGGCCAATATCCGTCGG
NM_182539.3         TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACATCCCAGGGCCAATATCCGTCGG
XM_005248872.1      TCAATCACACCAGTCCCTGCAAAGTCCAGGAACCCACATCCCAGGGCCAATATCCGTCGG
                    *************************************.**********************

HsCD00043528        ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
NM_182539.3         ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
XM_005248872.1      ATGCGCCGGATCATTGCTGAGGATCCTGAGTGGTCACTGGCCATCGTGCCCCTCCTCACA
                    ************************************************************

HsCD00043528        GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
NM_182539.3         GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
XM_005248872.1      GAGCTCTGCATTCAGCACATTATCAGGAACTTCCAGAAAAACCCTATCCTGAAGCAGATG
                    ************************************************************

HsCD00043528        CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
NM_182539.3         CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
XM_005248872.1      CTCCCGGAACACCAGCAGAAGGTCCTGAACCACCTGTCCCCTGACCTACCACTGGCTGTG
                    ************************************************************

HsCD00043528        ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
NM_182539.3         ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
XM_005248872.1      ACCGCCAACCTGATAGACAGTGAGAACTACTGGCTCCGCTGCTGCATGCATCGCTGGCCC
                    ************************************************************

HsCD00043528        GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
NM_182539.3         GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
XM_005248872.1      GTGTGCCACGTGGCCCACCATGGCGGCAGCTGGAAACGCATGTTCTTCGAGCGGCACCTG
                    ************************************************************

HsCD00043528        GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
NM_182539.3         GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
XM_005248872.1      GAGAACCTGCTAAAGCACTTTATCCCAGGCACCACAGACCCTGCGGTGATCCTCGACCTG
                    ************************************************************

HsCD00043528        CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
NM_182539.3         CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
XM_005248872.1      CTGCCGCTCTGCCGGAATTACGTGCGCAGGGTCCACGTCGATCAGTTCCTTCCGCCGGTG
                    ************************************************************

HsCD00043528        CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
NM_182539.3         CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
XM_005248872.1      CAGCTCCCGGCCCAGCTCCGGCCGGGCGACCAGTCCGACTCAGGCAGCGAGGGAGAGATG
                    ************************************************************

HsCD00043528        GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
NM_182539.3         GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
XM_005248872.1      GAGGAGCCCACCGTTGACCACTACCAACTGGGCGATCTGGTAGCTGGCCTGAGCCACCTG
                    ************************************************************

HsCD00043528        GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
NM_182539.3         GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
XM_005248872.1      GAGGAGCTGGACCTGGTGTACGATGTCAAGGACTGCGGCATGAATTTCGAGTGGAATCTC
                    ************************************************************

HsCD00043528        TTCCTCTTCACCTACCGCGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
NM_182539.3         TTCCTCTTCACCTACCGTGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
XM_005248872.1      TTCCTCTTCACCTACCGTGACTGCCTCTCCTTGGCAGCCGCCATCAAGGCATGCCACACC
                    ***************** ******************************************

HsCD00043528        CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
NM_182539.3         CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
XM_005248872.1      CTCAAGATCTTCAAGCTGACCCGAAGCAAGGTGGATGATGACAAGGCACGCATCATAATT
                    ************************************************************

HsCD00043528        CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
NM_182539.3         CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
XM_005248872.1      CGAAGCCTTCTGGACCACCCAGTCCTCGAGGAGCTGGACTTGTCACAAAACCTCATTGGA
                    ************************************************************

HsCD00043528        GACCGTGGTGCACGAGGCGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
NM_182539.3         GACCGTGGTGCACGAGGTGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
XM_005248872.1      GACCGTGGTGCACGAGGTGCTGCCAAGCTGCTGAGCCACAGCCGCCTGCGTGTGCTCAAC
                    ***************** ******************************************

HsCD00043528        CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
NM_182539.3         CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
XM_005248872.1      CTGGCTAACAACCAGGTGCGTGCACCCGGTGCCCAGTCCCTGGCTCACGCTCTGGCACAC
                    ************************************************************

HsCD00043528        AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
NM_182539.3         AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
XM_005248872.1      AACACCAACCTCATTTCCCTCAACCTACGTCTCAACTGCATCGAGGATGAGGGTGGCCAG
                    ************************************************************

HsCD00043528        GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
NM_182539.3         GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
XM_005248872.1      GCTCTTGCCCATGCCTTGCAGACCAACAAGTGCCTCACCACGCTGCACCTCGGTGGCAAT
                    ************************************************************

HsCD00043528        GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
NM_182539.3         GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
XM_005248872.1      GAGCTGTCTGAGCCCACCGCCACACTCCTGTCACAGGTGCTCGCCATCAACACCACACTC
                    ************************************************************

HsCD00043528        ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
NM_182539.3         ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
XM_005248872.1      ACCAGCATCAACCTGTCCTGCAACCACATCGGGCTGGACGGTGGGAAGCAGCTCCTGGAA
                    ************************************************************

HsCD00043528        GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
NM_182539.3         GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
XM_005248872.1      GGCATGTCAGACAACAAGACCCTCCTGGAATTTGACTTGCGCCTGTCAGATGTGGCCCAG
                    ************************************************************

HsCD00043528        GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
NM_182539.3         GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
XM_005248872.1      GAAAGCGAGTACCTCATTGGCCAGGCCCTCTACGCAAACCGAGAAGCAGCCCGCCAGCGG
                    ************************************************************

HsCD00043528        GCCCTGAATCCCAGCCACTTCATGTCAACCATCACTGCCAATGGCCCTGAGAACTCTGTG
NM_182539.3         GCCCTGAATCCCAGCCACTTCATGTCAACCATAACTGCCAATGGCCCTGAGAACTCTGTG
XM_005248872.1      GCCCTGAATCCCAGCCACTTCATGTCAACCATAACTGCCAATGGCCCTGAGAACTCTGTG
                    ********************************.***************************

HsCD00043528        GGATAG
NM_182539.3         GGATAA
XM_005248872.1      GGATAA
                    *****.

Protein

SMART domain : LRR_RI : Leucine rich repeat, ribonuclease inhibitor type
UniProt : Q5JU00
HPRD : 14568

Clone

Cloning Information : 177608
HIP Master Clone ID : 152753
Original Clone ID : FLH177608.01X

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 6
Map Location : 6p21.1

Reagents

Labome : TCTE1-antibody