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GRPEL1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GRPEL1
Gene Name:  GrpE-like 1, mitochondrial (E. coli)
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177704.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043540

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 654nts         Open reading frame : 1 to 654
Reference Sequence Alignment

ATGGCGGCTCAGTGCGTGAGGTTGGCGCGGCGCAGTCTTCCTGCTTTGGCGTTGTCTCTC
AGGCCATCTCCCCGGTTGTTGTGCACAGCCACGAAACAAAAGAACAGTGGCCAGAACCTG
GAAGAGGACATGGGTCAGAGTGAACAGAAGGCAGATCCTCCTGCTACAGAGAAGACCCTC
CTGGAAGAGAAGGTCAAGTTGGAGGAACAGCTGAAGGAGACTGTGGAAAAATATAAACGA
GCTTTGGCAGACACTGAGAACTTACGGCAGAGGAGCCAGAAATTGGTGGAGGAGGCAAAA
TTATACGGCATTCAAGCCTTCTGCAAGGACTTGTTGGAGGTGGCAGACGTTCTGGAGAAG
GCAACACAGTGTGTTCCAAAAGAAGAAATTAAAGACGATAACCCTCACCTGAAGAACCTC
TATGAGGGGCTGGTCATGACTGAAGTCCAGATCCAGAAGGTGTTCACAAAGCATGGCTTG
CTCAAGTTGAACCCTGTCGGAGCCAAGTTCGACCCTTATGAACATGAGGCCTTGTTCCAC
ACACCGGTTGAGGGGAAGGAGCCAGGCACAGTGGCCCTAGTTAGCAAAGTGGGGTACAAG
CTGCATGGGCGCACTCTGAGACCCGCCCTGGTGGGGGTGGTGAAGGAAGCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 700
Start on reference sequence 47
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GRPEL1
Symbol Nomenclature : GRPEL1
SYNONYM : HMGE
Designation : GrpE-like protein cochaperone|grpE protein homolog 1, mitochondrial|mt-GrpE#1
Full Nomenclature : GrpE-like 1, mitochondrial (E. coli)
GENEID : 80273
HGNC : 19696
MIM : 606173
Target GenBank: BC024242

 

Reference Sequence Alignment

  

HsCD00043540      ATGGCGGCTCAGTGCGTGAGGTTGGCGCGGCGCAGTCTTCCTGCTTTGGCGTTGTCTCTC
NM_025196.2       ATGGCGGCTCAGTGCGTGAGGTTGGCGCGGCGCAGTCTTCCTGCTTTGGCGTTGTCTCTC
                  ************************************************************

HsCD00043540      AGGCCATCTCCCCGGTTGTTGTGCACAGCCACGAAACAAAAGAACAGTGGCCAGAACCTG
NM_025196.2       AGGCCATCTCCCCGGTTGTTGTGCACAGCCACGAAACAAAAGAACAGTGGCCAGAACCTG
                  ************************************************************

HsCD00043540      GAAGAGGACATGGGTCAGAGTGAACAGAAGGCAGATCCTCCTGCTACAGAGAAGACCCTC
NM_025196.2       GAAGAGGACATGGGTCAGAGTGAACAGAAGGCAGATCCTCCTGCTACAGAGAAGACCCTC
                  ************************************************************

HsCD00043540      CTGGAAGAGAAGGTCAAGTTGGAGGAACAGCTGAAGGAGACTGTGGAAAAATATAAACGA
NM_025196.2       CTGGAAGAGAAGGTCAAGTTGGAGGAACAGCTGAAGGAGACTGTGGAAAAATATAAACGA
                  ************************************************************

HsCD00043540      GCTTTGGCAGACACTGAGAACTTACGGCAGAGGAGCCAGAAATTGGTGGAGGAGGCAAAA
NM_025196.2       GCTTTGGCAGACACTGAGAACTTACGGCAGAGGAGCCAGAAATTGGTGGAGGAGGCAAAA
                  ************************************************************

HsCD00043540      TTATACGGCATTCAAGCCTTCTGCAAGGACTTGTTGGAGGTGGCAGACGTTCTGGAGAAG
NM_025196.2       TTATACGGCATTCAAGCCTTCTGCAAGGACTTGTTGGAGGTGGCAGACGTTCTGGAGAAG
                  ************************************************************

HsCD00043540      GCAACACAGTGTGTTCCAAAAGAAGAAATTAAAGACGATAACCCTCACCTGAAGAACCTC
NM_025196.2       GCAACACAGTGTGTTCCAAAAGAAGAAATTAAAGACGATAACCCTCACCTGAAGAACCTC
                  ************************************************************

HsCD00043540      TATGAGGGGCTGGTCATGACTGAAGTCCAGATCCAGAAGGTGTTCACAAAGCATGGCTTG
NM_025196.2       TATGAGGGGCTGGTCATGACTGAAGTCCAGATCCAGAAGGTGTTCACAAAGCATGGCTTG
                  ************************************************************

HsCD00043540      CTCAAGTTGAACCCTGTCGGAGCCAAGTTCGACCCTTATGAACATGAGGCCTTGTTCCAC
NM_025196.2       CTCAAGTTGAACCCTGTCGGAGCCAAGTTCGACCCTTATGAACATGAGGCCTTGTTCCAC
                  ************************************************************

HsCD00043540      ACACCGGTTGAGGGGAAGGAGCCAGGCACAGTGGCCCTAGTTAGCAAAGTGGGGTACAAG
NM_025196.2       ACACCGGTTGAGGGGAAGGAGCCAGGCACAGTGGCCCTAGTTAGCAAAGTGGGGTACAAG
                  ************************************************************

HsCD00043540      CTGCATGGGCGCACTCTGAGACCCGCCCTGGTGGGGGTGGTGAAGGAAGCTTAG
NM_025196.2       CTGCATGGGCGCACTCTGAGACCCGCCCTGGTGGGGGTGGTGAAGGAAGCTTAG
                  ******************************************************

Pathways

Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

UniProt : Q9HAV7
HPRD : 07550

Clone

Cloning Information : 177704
HIP Master Clone ID : 152849
Original Clone ID : FLH177704.01X

Organism


TAX_ID : 9606
Species Specific ID: 80273

Genome

Chromosome : 4
Map Location : 4p16

Reagents

Labome : GRPEL1-antibody