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CAPN6 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN6
Gene Name:  calpain 6
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH180606.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00043548

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1926nts         Open reading frame : 1 to 1926
Reference Sequence Alignment

ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
CTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1955
Start on reference sequence 30
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN6
Symbol Nomenclature : CAPN6
SYNONYM : CANPX; CAPNX; CalpM; DJ914P14.1
Designation : calpain-6|calpain-like protease X-linked|calpamodulin
Full Nomenclature : calpain 6
GENEID : 827
HGNC : 1483
MIM : 300146
Vega : OTTHUMG00000022203
Target GenBank: BC000730

 

Reference Sequence Alignment

  

HsCD00043548      ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
NM_014289.3       ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
                  ************************************************************

HsCD00043548      ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
NM_014289.3       ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
                  ************************************************************

HsCD00043548      TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
NM_014289.3       TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
                  ************************************************************

HsCD00043548      CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
NM_014289.3       CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
                  ************************************************************

HsCD00043548      AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
NM_014289.3       AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
                  ************************************************************

HsCD00043548      ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
NM_014289.3       ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
                  ************************************************************

HsCD00043548      TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
NM_014289.3       TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
                  ************************************************************

HsCD00043548      CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
NM_014289.3       CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
                  ************************************************************

HsCD00043548      GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
NM_014289.3       GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
                  ************************************************************

HsCD00043548      ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
NM_014289.3       ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
                  ************************************************************

HsCD00043548      AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
NM_014289.3       AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
                  ************************************************************

HsCD00043548      ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
NM_014289.3       ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
                  ************************************************************

HsCD00043548      GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
NM_014289.3       GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
                  ************************************************************

HsCD00043548      AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
NM_014289.3       AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
                  ************************************************************

HsCD00043548      CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
NM_014289.3       CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
                  ************************************************************

HsCD00043548      GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
NM_014289.3       GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
                  ************************************************************

HsCD00043548      GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
NM_014289.3       GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
                  ************************************************************

HsCD00043548      CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
NM_014289.3       CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
                  ************************************************************

HsCD00043548      ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
NM_014289.3       ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
                  ************************************************************

HsCD00043548      TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
NM_014289.3       TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
                  ************************************************************

HsCD00043548      TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
NM_014289.3       TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
                  ************************************************************

HsCD00043548      ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
NM_014289.3       ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
                  ************************************************************

HsCD00043548      ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
NM_014289.3       ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
                  ************************************************************

HsCD00043548      TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
NM_014289.3       TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
                  ************************************************************

HsCD00043548      GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
NM_014289.3       GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
                  ************************************************************

HsCD00043548      ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
NM_014289.3       ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
                  ************************************************************

HsCD00043548      ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
NM_014289.3       ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
                  ************************************************************

HsCD00043548      TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
NM_014289.3       TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
                  ************************************************************

HsCD00043548      GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
NM_014289.3       GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
                  ************************************************************

HsCD00043548      CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
NM_014289.3       CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
                  ************************************************************

HsCD00043548      GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
NM_014289.3       GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
                  ************************************************************

HsCD00043548      GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
NM_014289.3       GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
                  ************************************************************

HsCD00043548      CTCTAG
NM_014289.3       CTCTAA
                  *****.

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 180606
HIP Master Clone ID : 155707
Original Clone ID : FLH180606.01X

Organism


TAX_ID : 9606
Species Specific ID: 827

Genome

Chromosome : X
Map Location : Xq23
Ensembl : ENSG00000077274

Reagents

Labome : CAPN6-antibody