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RNF41 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RNF41
Gene Name:  ring finger protein 41
Sequence             
Original Clone ID: ccsbBroad304_02346
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00435610

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 952nts         Open reading frame : 1 to 952
Reference Sequence Alignment

ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNF41
Symbol Nomenclature : RNF41
SYNONYM : FLRF; NRDP1
Designation : E3 ubiquitin-protein ligase NRDP1|fetal liver ring finger|neuregulin receptor degradation protein-1
Full Nomenclature : ring finger protein 41
GENEID : 10193
Locus Tag : SBBI03
GenBank Accession : EU446758
HGNC : 18401
Target GenBank: EU446758

 

Reference Sequence Alignment

  

HsCD00435610        ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_001242826.1      ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_005785.3         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_194359.2         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
                    ************************************************************

HsCD00435610        TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_001242826.1      TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_005785.3         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_194359.2         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
                    ************************************************************

HsCD00435610        GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_001242826.1      GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_005785.3         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_194359.2         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
                    ************************************************************

HsCD00435610        ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_001242826.1      ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_005785.3         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_194359.2         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
                    ************************************************************

HsCD00435610        ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_001242826.1      ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_005785.3         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_194359.2         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
                    ************************************************************

HsCD00435610        TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_001242826.1      TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_005785.3         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_194359.2         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
                    ************************************************************

HsCD00435610        GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_001242826.1      GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_005785.3         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_194359.2         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
                    ************************************************************

HsCD00435610        GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_001242826.1      GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_005785.3         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_194359.2         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
                    ************************************************************

HsCD00435610        CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_001242826.1      CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_005785.3         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_194359.2         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
                    ************************************************************

HsCD00435610        CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_001242826.1      CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_005785.3         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_194359.2         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
                    ************************************************************

HsCD00435610        GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_001242826.1      GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_005785.3         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_194359.2         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
                    ************************************************************

HsCD00435610        CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_001242826.1      CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_005785.3         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_194359.2         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
                    ************************************************************

HsCD00435610        TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_001242826.1      TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_005785.3         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_194359.2         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
                    ************************************************************

HsCD00435610        ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_001242826.1      ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_005785.3         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_194359.2         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
                    ************************************************************

HsCD00435610        CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_001242826.1      CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_005785.3         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_194359.2         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
                    ************************************************************

HsCD00435610        GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATA---
NM_001242826.1      GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_005785.3         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_194359.2         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
                    ***************************************************   

Pathways

NCI : ErbB2/ErbB3 signaling events
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Downregulation of ERBB2:ERBB3 signaling
Reactome : Immune System
Reactome : Signal Transduction
Reactome : Signaling by ERBB2
WikiPathway : Regulation of toll-like receptor signaling pathway

Protein

SMART domain : Ubox : Modified RING finger domain
SMART domain : RING : Ring finger
HPRD : 11520

Clone

Original Clone ID : ccsbBroad304_02346

Organism


TAX_ID : 9606
Species Specific ID: 10193

Genome

Chromosome : 12
Map Location : 12q13.13

Reagents

Labome : RNF41-antibody