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OPN5 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN5
Gene Name:  opsin 5
Sequence             
Original Clone ID: ccsbBroad304_14439
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00437112

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 568nts         Open reading frame : 1 to 568
Reference Sequence Alignment

ATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGAACCTCGTGCACCCTG
GACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTCCATCCTGAACATCCTCTTC
TTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTAAAGATCATTGCCAAG
GTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATCCATAGCAGCCATGTG
CTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGATTCCTGATTGCCTGG
ATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCAGACTCCATTCCCATA
CAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATGTACAATCCCATCATT
TACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGTTTGAAAGCAACCAAG
AAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTCAGGAAGTCTTCTGCT
GTGCTGGAAATTCATGAAGAGTGGGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN5
Symbol Nomenclature : OPN5
SYNONYM : GPR136; PGR12; TMEM13
Designation : G-protein coupled receptor 136|neuropsin|opsin-5|transmembrane protein 13
Full Nomenclature : opsin 5
GENEID : 221391
HGNC : 19992
MIM : 609042
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00437112      ------------------------------------------------------------
NM_181744.3       ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
                                                                              

HsCD00437112      ------------------------------------------------------------
NM_181744.3       GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
                                                                              

HsCD00437112      ---------------ATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
NM_181744.3       TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
                                 *********************************************

HsCD00437112      ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTCCATC
NM_181744.3       ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
                  ******************************************************* ****

HsCD00437112      CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
NM_181744.3       CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
                  ************************************************************

HsCD00437112      AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
NM_181744.3       AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
                  ************************************************************

HsCD00437112      CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
NM_181744.3       CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
                  ************************************************************

HsCD00437112      TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
NM_181744.3       TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
                  ************************************************************

HsCD00437112      GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
NM_181744.3       GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
                  ************************************************************

HsCD00437112      TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
NM_181744.3       TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
                  ************************************************************

HsCD00437112      TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
NM_181744.3       TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
                  ************************************************************

HsCD00437112      AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA---
NM_181744.3       AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAATAA
                  ******************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

HPRD : 12358

Clone

Original Clone ID : ccsbBroad304_14439

Organism


TAX_ID : 9606
Species Specific ID: 221391

Genome

Chromosome : 6
Map Location : 6p12.3

Reagents

Labome : OPN5-antibody