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PEPD (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PEPD
Gene Name:  peptidase D
Sequence             
Original Clone ID: ccsbBroad304_10441
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00438302

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1480nts         Open reading frame : 1 to 1480
Reference Sequence Alignment

ATGGCGGCGGCCACCTGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTG
GCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTG
CAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGAC
ACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCA
GGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTT
CCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAG
TATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG
CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAG
GCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATC
GTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAA
ATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATAT
GAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCC
TACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGA
GCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG
TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCA
GACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATG
AAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAG
CTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGG
GCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGA
GGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCA
CGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC
CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCTTTAACCGCGAGGTCCTG
CAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGC
GGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCA
GGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCCAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
2 errors; 2 non-synonomous errors;
 early stop codon
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: EvNO00877063
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PEPD
Symbol Nomenclature : PEPD
SYNONYM : PROLIDASE
Designation : X-Pro dipeptidase|aminoacyl-L-proline hydrolase|imidodipeptidase|proline dipeptidase|xaa-Pro dipeptidase
Full Nomenclature : peptidase D
GENEID : 5184
HGNC : 8840
MIM : 613230
Vega : OTTHUMG00000180470
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00438302        ATGGCGGCGGCCACCTGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTG
NM_000285.3         ATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTG
NM_001166056.1      ATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTG
                    *************** ********************************************

HsCD00438302        GCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTG
NM_000285.3         GCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTG
NM_001166056.1      GCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTG
                    ************************************************************

HsCD00438302        CAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGAC
NM_000285.3         CAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGAC
NM_001166056.1      CAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGAC
                    ************************************************************

HsCD00438302        ACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCA
NM_000285.3         ACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCA
NM_001166056.1      ACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCA
                    ************************************************************

HsCD00438302        GGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTT
NM_000285.3         GGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTT
NM_001166056.1      GGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTT
                    ************************************************************

HsCD00438302        CCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAG
NM_000285.3         CCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAG
NM_001166056.1      CCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTTCAAGGAGAAG
                    ************************************************************

HsCD00438302        TATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG
NM_000285.3         TATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG
NM_001166056.1      TATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG
                    ************************************************************

HsCD00438302        CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAG
NM_000285.3         CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAG
NM_001166056.1      CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAG
                    ************************************************************

HsCD00438302        GCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATC
NM_000285.3         GCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATC
NM_001166056.1      GCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATC
                    ************************************************************

HsCD00438302        GTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAA
NM_000285.3         GTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAA
NM_001166056.1      GTTGAGT-----------------------------------------------------
                    *******                                                     

HsCD00438302        ATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATAT
NM_000285.3         ATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAGTGGGAATGAAAGAATAT
NM_001166056.1      ------------------------------------------------------------
                                                                                

HsCD00438302        GAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCC
NM_000285.3         GAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCC
NM_001166056.1      ----------GCCTCTTCGAGCACTACTGCTACTCCCGGGGCGGCATGCGCCACAGCTCC
                              **************************************************

HsCD00438302        TACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGA
NM_000285.3         TACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGA
NM_001166056.1      TACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGGACACGCCGGA
                    ************************************************************

HsCD00438302        GCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG
NM_000285.3         GCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG
NM_001166056.1      GCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG
                    ************************************************************

HsCD00438302        TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCA
NM_000285.3         TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCA
NM_001166056.1      TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCA
                    ************************************************************

HsCD00438302        GACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATG
NM_000285.3         GACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATG
NM_001166056.1      GACCAGAAGGCCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATG
                    ************************************************************

HsCD00438302        AAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAG
NM_000285.3         AAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAG
NM_001166056.1      AAGCCAGGTGTCTGGTGGCCTGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAG
                    ************************************************************

HsCD00438302        CTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGG
NM_000285.3         CTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGG
NM_001166056.1      CTGGCCCACATGGGCATCCTGAGCGGCAGCGTGGACGCCATGGTCCAGGCTCACCTGGGG
                    ************************************************************

HsCD00438302        GCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGA
NM_000285.3         GCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGA
NM_001166056.1      GCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCATTGACGTGCACGACGTGGGA
                    ************************************************************

HsCD00438302        GGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCA
NM_000285.3         GGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCA
NM_001166056.1      GGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCA
                    ************************************************************

HsCD00438302        CGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC
NM_000285.3         CGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC
NM_001166056.1      CGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC
                    ************************************************************

HsCD00438302        CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCTTTAACCGCGAGGTCCTG
NM_000285.3         CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTG
NM_001166056.1      CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTG
                    ****************************************** *****************

HsCD00438302        CAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGC
NM_000285.3         CAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGC
NM_001166056.1      CAGCGCTTTCGCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGC
                    ************************************************************

HsCD00438302        GGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCA
NM_000285.3         GGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCA
NM_001166056.1      GGCATAGAGCTGCTGACCTGCGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCA
                    ************************************************************

HsCD00438302        GGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCCAG---
NM_000285.3         GGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCAAGTAG
NM_001166056.1      GGCTGTGACAAGGCCTTTACCCCCTTCTCTGGCCCCAAGTAG
                    ************************************.**   

Protein

SMART domain : AMP_N : Aminopeptidase P, N-terminal domain
HPRD : 01358

Clone

Original Clone ID : ccsbBroad304_10441

Organism


TAX_ID : 9606
Species Specific ID: 5184

Genome

Chromosome : 19
Map Location : 19q13.11
Ensembl : ENSG00000124299

Reagents

Labome : PEPD-antibody