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SSTR4 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SSTR4
Gene Name:  somatostatin receptor 4
Sequence             
Original Clone ID: ccsbBroad304_01603
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23430259
Title: Role of C-terminal Membrane-Proximal Basic Residues in Cell Surface Trafficking of HIV Coreceptor GPR15
PMID: 23430259
Title: Role of C-terminal Membrane-Proximal Basic Residues in Cell Surface Trafficking of HIV Coreceptor GPR15
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00440165

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1165nts         Open reading frame : 1 to 1165
Reference Sequence Alignment

ATGAGCGCCCCCTCGACGCTGCCCCCCGGGGGCGAGGAAGGGCTGGGGACGGCCTGGCCC
TCTGCAGCCAATGCCAGTAGCGCTCCGGCGGAGGCGGAGGAGGCGGTGGCGGGGCCCGGG
GACGCGCGGGCGGCGGGCATGGTCGCTATCCAGTGCATCTACGCGCTGGTGTGCCTGGTG
GGGCTGGTGGGCAACGCCCTGGTCATCTTCGTGATCCTTCGCTACGCCAAGATGAAGACG
GCTACCAACATCTACCTGCTCAACCTGGCCGTAGCCGACGAGCTCTTCATGCTGAGCGTG
CCCTTCGTGGCCTCGTCGGCCGCCCTGCGCCACTGGCCCTTCGGCTCCGTGCTGTGCCGC
GCGGTGCTCAGCGTCGACGGCCTCAACATGTTCACCAGCGTCTTCTGTCTCACCGTGCTC
AGCGTGGACCGCTACGTGGCCGTGGTGCACCCTCTGCGCGCGGCGACCTACCGGCGGCCC
AGCGTGGCCAAGCTCATCAACCTGGGCGTGTGGCTGGCATCCCTGTTGGTCACTCTCCCC
ATCGCCATCTTCGCAGACACCAGACCGGCTCGCGGCGGCCAGGCCGTGGCCTGCAACCTG
CAGTGGCCACACCCGGCCTGGTCGGCAGTCTTCGTGGTCTACACTTTCCTGCTGGGCTTC
CTGCTGCCCGTGCTGGCCATTGGCCTGTGCTACCTGCTCATCGTGGGCAAGATGCGCGCC
GTGGCCCTGCGCGCTGGCTGGCAGCAGCGCAGGCGCTCGGAGAAGAAAATCACCAGGCTG
GTGCTGATGGTCGTGGTCGTCTTTGTGCTCTGCTGGATGCCTTTCTACGTGGTGCAGCTG
CTGAACCTCTTCGTGACCAGCCTTGATGCCACCGTCAACCACGTGTCCCTTATCCTTAGC
TATGCCAACAGCTGCGCCAACCCCATTCTCTATGGCTTCCTCTCCGACAACTTCCGCCGA
TTCTTCCAGCGGGTTCTCTGCCTGCGCTGCTGCCTCCTGGAAGGTGCTGGAGGTGCTGAG
GAGGAGCCCCTGGACTACTATGCCACTGCTCTCAAGAGCAAAGGTGGGGCAGGGTGCATG
TGCCCCCCACTCCCCTGCCAGCAGGAAGCCCTGCAACCAGAACCCGGCCGCAAGCGCATC
CCCCTCACCAGGACCACCACCTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SSTR4
Symbol Nomenclature : SSTR4
SYNONYM : SS-4-R; SS4-R; SS4R
Designation : G-protein coupled receptor|somatostatin receptor type 4
Full Nomenclature : somatostatin receptor 4
GENEID : 6754
HGNC : 11333
MIM : 182454
Vega : OTTHUMG00000032054
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00440165      ATGAGCGCCCCCTCGACGCTGCCCCCCGGGGGCGAGGAAGGGCTGGGGACGGCCTGGCCC
NM_001052.2       ATGAGCGCCCCCTCGACGCTGCCCCCCGGGGGCGAGGAAGGGCTGGGGACGGCCTGGCCC
                  ************************************************************

HsCD00440165      TCTGCAGCCAATGCCAGTAGCGCTCCGGCGGAGGCGGAGGAGGCGGTGGCGGGGCCCGGG
NM_001052.2       TCTGCAGCCAATGCCAGTAGCGCTCCGGCGGAGGCGGAGGAGGCGGTGGCGGGGCCCGGG
                  ************************************************************

HsCD00440165      GACGCGCGGGCGGCGGGCATGGTCGCTATCCAGTGCATCTACGCGCTGGTGTGCCTGGTG
NM_001052.2       GACGCGCGGGCGGCGGGCATGGTCGCTATCCAGTGCATCTACGCGCTGGTGTGCCTGGTG
                  ************************************************************

HsCD00440165      GGGCTGGTGGGCAACGCCCTGGTCATCTTCGTGATCCTTCGCTACGCCAAGATGAAGACG
NM_001052.2       GGGCTGGTGGGCAACGCCCTGGTCATCTTCGTGATCCTTCGCTACGCCAAGATGAAGACG
                  ************************************************************

HsCD00440165      GCTACCAACATCTACCTGCTCAACCTGGCCGTAGCCGACGAGCTCTTCATGCTGAGCGTG
NM_001052.2       GCTACCAACATCTACCTGCTCAACCTGGCCGTAGCCGACGAGCTCTTCATGCTGAGCGTG
                  ************************************************************

HsCD00440165      CCCTTCGTGGCCTCGTCGGCCGCCCTGCGCCACTGGCCCTTCGGCTCCGTGCTGTGCCGC
NM_001052.2       CCCTTCGTGGCCTCGTCGGCCGCCCTGCGCCACTGGCCCTTCGGCTCCGTGCTGTGCCGC
                  ************************************************************

HsCD00440165      GCGGTGCTCAGCGTCGACGGCCTCAACATGTTCACCAGCGTCTTCTGTCTCACCGTGCTC
NM_001052.2       GCGGTGCTCAGCGTCGACGGCCTCAACATGTTCACCAGCGTCTTCTGTCTCACCGTGCTC
                  ************************************************************

HsCD00440165      AGCGTGGACCGCTACGTGGCCGTGGTGCACCCTCTGCGCGCGGCGACCTACCGGCGGCCC
NM_001052.2       AGCGTGGACCGCTACGTGGCCGTGGTGCACCCTCTGCGCGCGGCGACCTACCGGCGGCCC
                  ************************************************************

HsCD00440165      AGCGTGGCCAAGCTCATCAACCTGGGCGTGTGGCTGGCATCCCTGTTGGTCACTCTCCCC
NM_001052.2       AGCGTGGCCAAGCTCATCAACCTGGGCGTGTGGCTGGCATCCCTGTTGGTCACTCTCCCC
                  ************************************************************

HsCD00440165      ATCGCCATCTTCGCAGACACCAGACCGGCTCGCGGCGGCCAGGCCGTGGCCTGCAACCTG
NM_001052.2       ATCGCCATCTTCGCAGACACCAGACCGGCTCGCGGCGGCCAGGCCGTGGCCTGCAACCTG
                  ************************************************************

HsCD00440165      CAGTGGCCACACCCGGCCTGGTCGGCAGTCTTCGTGGTCTACACTTTCCTGCTGGGCTTC
NM_001052.2       CAGTGGCCACACCCGGCCTGGTCGGCAGTCTTCGTGGTCTACACTTTCCTGCTGGGCTTC
                  ************************************************************

HsCD00440165      CTGCTGCCCGTGCTGGCCATTGGCCTGTGCTACCTGCTCATCGTGGGCAAGATGCGCGCC
NM_001052.2       CTGCTGCCCGTGCTGGCCATTGGCCTGTGCTACCTGCTCATCGTGGGCAAGATGCGCGCC
                  ************************************************************

HsCD00440165      GTGGCCCTGCGCGCTGGCTGGCAGCAGCGCAGGCGCTCGGAGAAGAAAATCACCAGGCTG
NM_001052.2       GTGGCCCTGCGCGCTGGCTGGCAGCAGCGCAGGCGCTCGGAGAAGAAAATCACCAGGCTG
                  ************************************************************

HsCD00440165      GTGCTGATGGTCGTGGTCGTCTTTGTGCTCTGCTGGATGCCTTTCTACGTGGTGCAGCTG
NM_001052.2       GTGCTGATGGTCGTGGTCGTCTTTGTGCTCTGCTGGATGCCTTTCTACGTGGTGCAGCTG
                  ************************************************************

HsCD00440165      CTGAACCTCTTCGTGACCAGCCTTGATGCCACCGTCAACCACGTGTCCCTTATCCTTAGC
NM_001052.2       CTGAACCTCTTCGTGACCAGCCTTGATGCCACCGTCAACCACGTGTCCCTTATCCTTAGC
                  ************************************************************

HsCD00440165      TATGCCAACAGCTGCGCCAACCCCATTCTCTATGGCTTCCTCTCCGACAACTTCCGCCGA
NM_001052.2       TATGCCAACAGCTGCGCCAACCCCATTCTCTATGGCTTCCTCTCCGACAACTTCCGCCGA
                  ************************************************************

HsCD00440165      TTCTTCCAGCGGGTTCTCTGCCTGCGCTGCTGCCTCCTGGAAGGTGCTGGAGGTGCTGAG
NM_001052.2       TTCTTCCAGCGGGTTCTCTGCCTGCGCTGCTGCCTCCTGGAAGGTGCTGGAGGTGCTGAG
                  ************************************************************

HsCD00440165      GAGGAGCCCCTGGACTACTATGCCACTGCTCTCAAGAGCAAAGGTGGGGCAGGGTGCATG
NM_001052.2       GAGGAGCCCCTGGACTACTATGCCACTGCTCTCAAGAGCAAAGGTGGGGCAGGGTGCATG
                  ************************************************************

HsCD00440165      TGCCCCCCACTCCCCTGCCAGCAGGAAGCCCTGCAACCAGAACCCGGCCGCAAGCGCATC
NM_001052.2       TGCCCCCCACTCCCCTGCCAGCAGGAAGCCCTGCAACCAGAACCCGGCCGCAAGCGCATC
                  ************************************************************

HsCD00440165      CCCCTCACCAGGACCACCACCTTC---
NM_001052.2       CCCCTCACCAGGACCACCACCTTCTGA
                  ************************   

Pathways

Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway
Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Peptide ligand-binding receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : Peptide GPCRs

Protein

HPRD : 01676

Clone

Original Clone ID : ccsbBroad304_01603

Organism


TAX_ID : 9606
Species Specific ID: 6754

Genome

Chromosome : 20
Map Location : 20p11.2
Ensembl : ENSG00000132671

Reagents

Labome : SSTR4-antibody