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LGALS12 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS12
Gene Name:  lectin, galactoside-binding, soluble, 12
Sequence             
Original Clone ID: ccsbBroad304_04477
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00441371

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1009nts         Open reading frame : 1 to 1009
Reference Sequence Alignment

ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAGGTTTCAGGTGGACTTC
CAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGCTTC
CATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGGGAG
GCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTCTTC
GGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTACCGG
CTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCTGTT
GGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACATCCT
TTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGTCTC
TCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTTACT
GTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCAGAC
AGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTCCTC
TTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAGCTG
GCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAGCTG
CGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS12
Symbol Nomenclature : LGALS12
SYNONYM : GAL12; GRIP1
Designation : galectin 12|galectin-12|galectin-related inhibitor of proliferation
Full Nomenclature : lectin, galactoside-binding, soluble, 12
GENEID : 85329
GenBank Accession : JF432638
HGNC : 15788
MIM : 606096
Vega : OTTHUMG00000167807
Target GenBank: JF432638

 

Reference Sequence Alignment

  

HsCD00441371        ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_001142535.1      ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_033101.3         ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_001142536.1      ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
                    ************************************************************

HsCD00441371        ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_001142535.1      ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_033101.3         ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_001142536.1      ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
                    ************************************************************

HsCD00441371        CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_001142535.1      CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_033101.3         CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_001142536.1      CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
                    ************************************************************

HsCD00441371        AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
NM_001142535.1      AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAGTAGGTTTCAGGTGGAC
NM_033101.3         AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
NM_001142536.1      AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
                    ********************************************   *************

HsCD00441371        TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_001142535.1      TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_033101.3         TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_001142536.1      TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
                    ************************************************************

HsCD00441371        TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_001142535.1      TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_033101.3         TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_001142536.1      TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
                    ************************************************************

HsCD00441371        GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_001142535.1      GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_033101.3         GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_001142536.1      GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
                    ************************************************************

HsCD00441371        TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_001142535.1      TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_033101.3         TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_001142536.1      TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
                    ************************************************************

HsCD00441371        CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_001142535.1      CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_033101.3         CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_001142536.1      CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
                    ************************************************************

HsCD00441371        GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_001142535.1      GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_033101.3         GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_001142536.1      GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTG-----
                    *******************************************************     

HsCD00441371        CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_001142535.1      CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_033101.3         CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_001142536.1      ----------------------GACATGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
                                          *.*: *********************************

HsCD00441371        CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_001142535.1      CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_033101.3         CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_001142536.1      CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
                    ************************************************************

HsCD00441371        ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_001142535.1      ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_033101.3         ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_001142536.1      ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
                    ************************************************************

HsCD00441371        GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_001142535.1      GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_033101.3         GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_001142536.1      GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
                    ************************************************************

HsCD00441371        CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_001142535.1      CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_033101.3         CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_001142536.1      CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
                    ************************************************************

HsCD00441371        CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_001142535.1      CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_033101.3         CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_001142536.1      CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
                    ************************************************************

HsCD00441371        CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCC---
NM_001142535.1      CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
NM_033101.3         CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
NM_001142536.1      CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
                    ***************************************************   

Protein

SMART domain : GLECT : Galectin
SMART domain : Gal-bind_lectin : Galactoside-binding lectin
HPRD : 16196

Clone

Original Clone ID : ccsbBroad304_04477

Organism


TAX_ID : 9606
Species Specific ID: 85329

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000133317

Reagents

Labome : LGALS12-antibody