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CPSF5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NUDT21
Gene Name:  nudix (nucleoside diphosphate linked moiety X)-type motif 21
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH183389.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00044205

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 684nts         Open reading frame : 1 to 684
Reference Sequence Alignment

ATGTCTGTGGTACCGCCCAATCGCTCGCAGACCGGCTGGCCCCGGGGGGTCACTCAGTTC
GGCAACAAGTACATCCAGCAGACGAAGCCCCTCACCCTGGAGCGCACCATCAACCTGTAC
CCTCTTACCAATTATACTTTTGGTACAAAAGAGCCCCTCTACGAGAAGGACAGCTCTGTT
GCAGCCAGATTTCAGCGCATGAGGGAAGAATTTGATAAAATTGGAATGAGGAGGACTGTA
GAAGGGGTTCTGATTGTACATGAGCACCGGCTACCCCATGTGTTACTGCTGCAGCTGGGA
ACAACTTTCTTCAAACTACCTGGTGGTGAACTTAACCCAGGAGAAGATGAAGTTGAAGGA
CTAAAACGCTTAATGACAGAGATACTGGGTCGTCAGGATGGAGTTTTGCAAGACTGGGTC
ATTGACGATTGCATTGGTAACTGGTGGAGACCAAATTTTGAACCTCCTCAGTATCCATAT
ATTCCTGCACATATTACAAAGCCTAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAA
GAAAAAGCCTTGTTTGCAGTCCCTAAAAATTACAAGCTGGTAGCTGCACCATTGTTTGAA
TTGTATGACAATGCACCAGGATATGGACCCATCATTTCTAGTCTCCCTCAGCTGTTGAGC
AGGTTCAATTTTATTTACAACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 801
Start on reference sequence 118
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NUDT21
Symbol Nomenclature : NUDT21
SYNONYM : CFIM25; CPSF5
Designation : CPSF 25 kDa subunit|cleavage and polyadenylation specific factor 5, 25 kD subunit|cleavage and polyadenylation specific factor 5, 25 kDa|cleavage and polyadenylation specificity factor 25 kDa subunit|cleavage and polyadenylation specificity factor subunit 5|cleavage factor Im complex 25 kDa subunit|nucleoside diphosphate-linked moiety X motif 21|nudix motif 21|pre-mRNA cleavage factor Im (25kD)|pre-mRNA cleavage factor Im 25 kDa subunit|pre-mRNA cleavage factor Im, 25kD subunit
Full Nomenclature : nudix (nucleoside diphosphate linked moiety X)-type motif 21
GENEID : 11051
HGNC : 13870
MIM : 604978
Vega : OTTHUMG00000133240
Target GenBank: BC001403

 

Reference Sequence Alignment

  

HsCD00044205      ATGTCTGTGGTACCGCCCAATCGCTCGCAGACCGGCTGGCCCCGGGGGGTCACTCAGTTC
NM_007006.2       ATGTCTGTGGTACCGCCCAATCGCTCGCAGACCGGCTGGCCCCGGGGGGTCACTCAGTTC
                  ************************************************************

HsCD00044205      GGCAACAAGTACATCCAGCAGACGAAGCCCCTCACCCTGGAGCGCACCATCAACCTGTAC
NM_007006.2       GGCAACAAGTACATCCAGCAGACGAAGCCCCTCACCCTGGAGCGCACCATCAACCTGTAC
                  ************************************************************

HsCD00044205      CCTCTTACCAATTATACTTTTGGTACAAAAGAGCCCCTCTACGAGAAGGACAGCTCTGTT
NM_007006.2       CCTCTTACCAATTATACTTTTGGTACAAAAGAGCCCCTCTACGAGAAGGACAGCTCTGTT
                  ************************************************************

HsCD00044205      GCAGCCAGATTTCAGCGCATGAGGGAAGAATTTGATAAAATTGGAATGAGGAGGACTGTA
NM_007006.2       GCAGCCAGATTTCAGCGCATGAGGGAAGAATTTGATAAAATTGGAATGAGGAGGACTGTA
                  ************************************************************

HsCD00044205      GAAGGGGTTCTGATTGTACATGAGCACCGGCTACCCCATGTGTTACTGCTGCAGCTGGGA
NM_007006.2       GAAGGGGTTCTGATTGTACATGAGCACCGGCTACCCCATGTGTTACTGCTGCAGCTGGGA
                  ************************************************************

HsCD00044205      ACAACTTTCTTCAAACTACCTGGTGGTGAACTTAACCCAGGAGAAGATGAAGTTGAAGGA
NM_007006.2       ACAACTTTCTTCAAACTACCTGGTGGTGAACTTAACCCAGGAGAAGATGAAGTTGAAGGA
                  ************************************************************

HsCD00044205      CTAAAACGCTTAATGACAGAGATACTGGGTCGTCAGGATGGAGTTTTGCAAGACTGGGTC
NM_007006.2       CTAAAACGCTTAATGACAGAGATACTGGGTCGTCAGGATGGAGTTTTGCAAGACTGGGTC
                  ************************************************************

HsCD00044205      ATTGACGATTGCATTGGTAACTGGTGGAGACCAAATTTTGAACCTCCTCAGTATCCATAT
NM_007006.2       ATTGACGATTGCATTGGTAACTGGTGGAGACCAAATTTTGAACCTCCTCAGTATCCATAT
                  ************************************************************

HsCD00044205      ATTCCTGCACATATTACAAAGCCTAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAA
NM_007006.2       ATTCCTGCACATATTACAAAGCCTAAGGAACATAAGAAGTTGTTTCTGGTTCAGCTTCAA
                  ************************************************************

HsCD00044205      GAAAAAGCCTTGTTTGCAGTCCCTAAAAATTACAAGCTGGTAGCTGCACCATTGTTTGAA
NM_007006.2       GAAAAAGCCTTGTTTGCAGTCCCTAAAAATTACAAGCTGGTAGCTGCACCATTGTTTGAA
                  ************************************************************

HsCD00044205      TTGTATGACAATGCACCAGGATATGGACCCATCATTTCTAGTCTCCCTCAGCTGTTGAGC
NM_007006.2       TTGTATGACAATGCACCAGGATATGGACCCATCATTTCTAGTCTCCCTCAGCTGTTGAGC
                  ************************************************************

HsCD00044205      AGGTTCAATTTTATTTACAACTAG
NM_007006.2       AGGTTCAATTTTATTTACAACTGA
                  **********************..

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of Intronless pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Processing of Capped Intronless Pre-mRNA
Reactome : Processing of Intronless Pre-mRNAs
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

UniProt : O43809
HPRD : 05400

Clone

Cloning Information : 183389
HIP Master Clone ID : 158333
Original Clone ID : FLH183389.01X

Organism


TAX_ID : 9606
Species Specific ID: 11051

Genome

Chromosome : 16
Map Location : 16q12.2
Ensembl : ENSG00000167005

Reagents

Labome : NUDT21-antibody