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GAN (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GAN
Gene Name:  gigaxonin
Sequence             
Original Clone ID: ccsbBroad304_01867
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442624

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1792nts         Open reading frame : 1 to 1792
Reference Sequence Alignment

ATGGCTGAGGGCAGTGCCGTGTCTGACCCTCAGCACGCCGCGCGTCTGCTGCGAGCGCTC
AGCTCTTTCCGCGAGGAGTCTCGCTTCTGCGACGCGCACCTGGTCCTCGACGGGGAGGAG
ATCCCGGTGCAGAAGAACATCCTGGCGGCGGCCAGCCCGTACATCAGGACAAAGTTAAAC
TATAATCCTCCAAAAGATGATGGATCAACTTATAAGATTGAACTTGAAGGGATATCGGTA
ATGGTTATGAGAGAGATCCTGGATTACATCTTCAGTGGGCAGATCAGGCTAAATGAAGAT
ACAATCCAAGATGTTGTTCAGGCAGCTGACCTGCTGCTACTGACGGACCTTAAAACCCTG
TGCTGTGAGTTTTTGGAAGGCTGCATTGCTGCTGAGAACTGTATTGGTATCCGTGACTTT
GCACTACATTACTGCCTCCATCACGTTCATTACCTTGCCACAGAATACCTGGAGACTCAT
TTCCGAGACGTCAGCAGCACGGAAGAATTCTTAGAGCTGAGTCCTCAAAAGCTTAAAGAA
GTGATTTCTCTTGAGAAGTTAAACGTTGGCAATGAAAGATATGTCTTTGAAGCAGTAATT
CGATGGATAGCACATGATACAGAAATAAGAAAGGTCCACATGAAGGATGTTATGTCAGCT
CTGTGGGTTTCAGGGTTGGACTCCAGTTATTTACGGGAACAGATGCTGAATGAACCATTA
GTACGAGAAATTGTCAAAGAGTGTAGCAATATACCGCTCAGCCAGCCGCAGCAAGGGGAG
GCGATGCTGGCCAACTTCAAACCCCGGGGCTACTCTGAGTGCATCGTGACTGTTGGTGGA
GAAGAGAGAGTTTCACGGAAACCCACAGCAGCGATGCGATGCATGTGCCCTCTCTATGAC
CCTAACAGGCAGCTTTGGATCGAACTGGCCCCTTTAAGCATGCCGAGAATTAACCATGGA
GTTCTCTCAGCAGAAGGATTTTTGTTTGTATTCGGGGGCCAAGATGAAAATAAGCAGACT
CTTAGCTCAGGAGAAAAGTATGATCCAGATGCAAATACATGGACAGCATTGCCACCTATG
AACGAGGCAAGACATAACTTCGGAATTGTGGAGATAGATGGGATGCTGTACATTTTGGGA
GGAGAGGATGGTGAAAAGGAGCTGATTTCCATGGAGTGTTACGATATTTATTCTAAAACC
TGGACAAAGCAACCTGATTTGACCATGGTCAGAAAGATCGGCTGCTATGCAGCTATGAAA
AAGAAAATCTACGCCATGGGTGGAGGCTCCTACGGAAAGCTTTTTGAGTCTGTAGAGTGT
TATGATCCCAGGACCCAGCAGTGGACTGCCATATGTCCACTAAAAGAGAGGAGGTTTGGA
GCGGTGGCCTGTGGAGTTGCTATGGAGCTGTATGTGTTTGGGGGAGTCCGAAGTCGTGAG
GACGCCCAGGGTAGCGAGATGGTAACTTGCAAGTCCGAGTTCTACCATGATGAGTTTAAA
AGGTGGATCTATCTTAACGACCAGAATTTATGCATCCCCGCCAGTTCCTCTTTTGTTTAT
GGAGCTGTACCTATAGGAGCCAGTATTTATGTTATTGGAGATCTTGATACAGGTACCAAT
TACGACTACGTGCGTGAGTTTAAAAGAAGCACAGGAACCTGGCACCACACTAAACCACTC
CTTCCATCCGACCTTCGCCGTACAGGATGTGCAGCCTTACGCATTGCGAATTGCAAGCTT
TTCCGCCTGCAGCTTCAGCAAGGCTTATTCCGTATTCGTGTTCATTCCCCT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GAN
Symbol Nomenclature : GAN
SYNONYM : GAN1; KLHL16
Designation : kelch-like family member 16|kelch-like protein 16
Full Nomenclature : gigaxonin
GENEID : 8139
GenBank Accession : HQ257897
HGNC : 4137
MIM : 605379
Target GenBank: HQ257897

 

Reference Sequence Alignment

  

HsCD00442624      ATGGCTGAGGGCAGTGCCGTGTCTGACCCTCAGCACGCCGCGCGTCTGCTGCGAGCGCTC
NM_022041.3       ATGGCTGAGGGCAGTGCCGTGTCTGACCCTCAGCACGCCGCGCGTCTGCTGCGAGCGCTC
                  ************************************************************

HsCD00442624      AGCTCTTTCCGCGAGGAGTCTCGCTTCTGCGACGCGCACCTGGTCCTCGACGGGGAGGAG
NM_022041.3       AGCTCTTTCCGCGAGGAGTCTCGCTTCTGCGACGCGCACCTGGTCCTCGACGGGGAGGAG
                  ************************************************************

HsCD00442624      ATCCCGGTGCAGAAGAACATCCTGGCGGCGGCCAGCCCGTACATCAGGACAAAGTTAAAC
NM_022041.3       ATCCCGGTGCAGAAGAACATCCTGGCGGCGGCCAGCCCGTACATCAGGACAAAGTTAAAC
                  ************************************************************

HsCD00442624      TATAATCCTCCAAAAGATGATGGATCAACTTATAAGATTGAACTTGAAGGGATATCGGTA
NM_022041.3       TATAATCCTCCAAAAGATGATGGATCAACTTATAAGATTGAACTTGAAGGGATATCGGTA
                  ************************************************************

HsCD00442624      ATGGTTATGAGAGAGATCCTGGATTACATCTTCAGTGGGCAGATCAGGCTAAATGAAGAT
NM_022041.3       ATGGTTATGAGAGAGATCCTGGATTACATCTTCAGTGGGCAGATCAGGCTAAATGAAGAT
                  ************************************************************

HsCD00442624      ACAATCCAAGATGTTGTTCAGGCAGCTGACCTGCTGCTACTGACGGACCTTAAAACCCTG
NM_022041.3       ACAATCCAAGATGTTGTTCAGGCAGCTGACCTGCTGCTACTGACGGACCTTAAAACCCTG
                  ************************************************************

HsCD00442624      TGCTGTGAGTTTTTGGAAGGCTGCATTGCTGCTGAGAACTGTATTGGTATCCGTGACTTT
NM_022041.3       TGCTGTGAGTTTTTGGAAGGCTGCATTGCTGCTGAGAACTGTATTGGTATCCGTGACTTT
                  ************************************************************

HsCD00442624      GCACTACATTACTGCCTCCATCACGTTCATTACCTTGCCACAGAATACCTGGAGACTCAT
NM_022041.3       GCACTACATTACTGCCTCCATCACGTTCATTACCTTGCCACAGAATACCTGGAGACTCAT
                  ************************************************************

HsCD00442624      TTCCGAGACGTCAGCAGCACGGAAGAATTCTTAGAGCTGAGTCCTCAAAAGCTTAAAGAA
NM_022041.3       TTCCGAGACGTCAGCAGCACGGAAGAATTCTTAGAGCTGAGTCCTCAAAAGCTTAAAGAA
                  ************************************************************

HsCD00442624      GTGATTTCTCTTGAGAAGTTAAACGTTGGCAATGAAAGATATGTCTTTGAAGCAGTAATT
NM_022041.3       GTGATTTCTCTTGAGAAGTTAAACGTTGGCAATGAAAGATATGTCTTTGAAGCAGTAATT
                  ************************************************************

HsCD00442624      CGATGGATAGCACATGATACAGAAATAAGAAAGGTCCACATGAAGGATGTTATGTCAGCT
NM_022041.3       CGATGGATAGCACATGATACAGAAATAAGAAAGGTCCACATGAAGGATGTTATGTCAGCT
                  ************************************************************

HsCD00442624      CTGTGGGTTTCAGGGTTGGACTCCAGTTATTTACGGGAACAGATGCTGAATGAACCATTA
NM_022041.3       CTGTGGGTTTCAGGGTTGGACTCCAGTTATTTACGGGAACAGATGCTGAATGAACCATTA
                  ************************************************************

HsCD00442624      GTACGAGAAATTGTCAAAGAGTGTAGCAATATACCGCTCAGCCAGCCGCAGCAAGGGGAG
NM_022041.3       GTACGAGAAATTGTCAAAGAGTGTAGCAATATACCGCTCAGCCAGCCGCAGCAAGGGGAG
                  ************************************************************

HsCD00442624      GCGATGCTGGCCAACTTCAAACCCCGGGGCTACTCTGAGTGCATCGTGACTGTTGGTGGA
NM_022041.3       GCGATGCTGGCCAACTTCAAACCCCGGGGCTACTCTGAGTGCATCGTGACTGTTGGTGGA
                  ************************************************************

HsCD00442624      GAAGAGAGAGTTTCACGGAAACCCACAGCAGCGATGCGATGCATGTGCCCTCTCTATGAC
NM_022041.3       GAAGAGAGAGTTTCACGGAAACCCACAGCAGCGATGCGATGCATGTGCCCTCTCTATGAC
                  ************************************************************

HsCD00442624      CCTAACAGGCAGCTTTGGATCGAACTGGCCCCTTTAAGCATGCCGAGAATTAACCATGGA
NM_022041.3       CCTAACAGGCAGCTTTGGATCGAACTGGCCCCTTTAAGCATGCCGAGAATTAACCATGGA
                  ************************************************************

HsCD00442624      GTTCTCTCAGCAGAAGGATTTTTGTTTGTATTCGGGGGCCAAGATGAAAATAAGCAGACT
NM_022041.3       GTTCTCTCAGCAGAAGGATTTTTGTTTGTATTCGGGGGCCAAGATGAAAATAAGCAGACT
                  ************************************************************

HsCD00442624      CTTAGCTCAGGAGAAAAGTATGATCCAGATGCAAATACATGGACAGCATTGCCACCTATG
NM_022041.3       CTTAGCTCAGGAGAAAAGTATGATCCAGATGCAAATACATGGACAGCATTGCCACCTATG
                  ************************************************************

HsCD00442624      AACGAGGCAAGACATAACTTCGGAATTGTGGAGATAGATGGGATGCTGTACATTTTGGGA
NM_022041.3       AACGAGGCAAGACATAACTTCGGAATTGTGGAGATAGATGGGATGCTGTACATTTTGGGA
                  ************************************************************

HsCD00442624      GGAGAGGATGGTGAAAAGGAGCTGATTTCCATGGAGTGTTACGATATTTATTCTAAAACC
NM_022041.3       GGAGAGGATGGTGAAAAGGAGCTGATTTCCATGGAGTGTTACGATATTTATTCTAAAACC
                  ************************************************************

HsCD00442624      TGGACAAAGCAACCTGATTTGACCATGGTCAGAAAGATCGGCTGCTATGCAGCTATGAAA
NM_022041.3       TGGACAAAGCAACCTGATTTGACCATGGTCAGAAAGATCGGCTGCTATGCAGCTATGAAA
                  ************************************************************

HsCD00442624      AAGAAAATCTACGCCATGGGTGGAGGCTCCTACGGAAAGCTTTTTGAGTCTGTAGAGTGT
NM_022041.3       AAGAAAATCTACGCCATGGGTGGAGGCTCCTACGGAAAGCTTTTTGAGTCTGTAGAGTGT
                  ************************************************************

HsCD00442624      TATGATCCCAGGACCCAGCAGTGGACTGCCATATGTCCACTAAAAGAGAGGAGGTTTGGA
NM_022041.3       TATGATCCCAGGACCCAGCAGTGGACTGCCATATGTCCACTAAAAGAGAGGAGGTTTGGA
                  ************************************************************

HsCD00442624      GCGGTGGCCTGTGGAGTTGCTATGGAGCTGTATGTGTTTGGGGGAGTCCGAAGTCGTGAG
NM_022041.3       GCGGTGGCCTGTGGAGTTGCTATGGAGCTGTATGTGTTTGGGGGAGTCCGAAGTCGTGAG
                  ************************************************************

HsCD00442624      GACGCCCAGGGTAGCGAGATGGTAACTTGCAAGTCCGAGTTCTACCATGATGAGTTTAAA
NM_022041.3       GACGCCCAGGGTAGCGAGATGGTAACTTGCAAGTCCGAGTTCTACCATGATGAGTTTAAA
                  ************************************************************

HsCD00442624      AGGTGGATCTATCTTAACGACCAGAATTTATGCATCCCCGCCAGTTCCTCTTTTGTTTAT
NM_022041.3       AGGTGGATCTATCTTAACGACCAGAATTTATGCATCCCCGCCAGTTCCTCTTTTGTTTAT
                  ************************************************************

HsCD00442624      GGAGCTGTACCTATAGGAGCCAGTATTTATGTTATTGGAGATCTTGATACAGGTACCAAT
NM_022041.3       GGAGCTGTACCTATAGGAGCCAGTATTTATGTTATTGGAGATCTTGATACAGGTACCAAT
                  ************************************************************

HsCD00442624      TACGACTACGTGCGTGAGTTTAAAAGAAGCACAGGAACCTGGCACCACACTAAACCACTC
NM_022041.3       TACGACTACGTGCGTGAGTTTAAAAGAAGCACAGGAACCTGGCACCACACTAAACCACTC
                  ************************************************************

HsCD00442624      CTTCCATCCGACCTTCGCCGTACAGGATGTGCAGCCTTACGCATTGCGAATTGCAAGCTT
NM_022041.3       CTTCCATCCGACCTTCGCCGTACAGGATGTGCAGCCTTACGCATTGCGAATTGCAAGCTT
                  ************************************************************

HsCD00442624      TTCCGCCTGCAGCTTCAGCAAGGCTTATTCCGTATTCGTGTTCATTCCCCT---
NM_022041.3       TTCCGCCTGCAGCTTCAGCAAGGCTTATTCCGTATTCGTGTTCATTCCCCTTGA
                  ***************************************************   

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
HPRD : 05647

Clone

Original Clone ID : ccsbBroad304_01867

Organism


TAX_ID : 9606
Species Specific ID: 8139

Genome

Chromosome : 16
Map Location : 16q24.1

Reagents

Labome : gigaxonin-antibody