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LRRC32 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LRRC32
Gene Name:  leucine rich repeat containing 32
Sequence             
Original Clone ID: ccsbBroad304_06255
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00442905

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1987nts         Open reading frame : 1 to 1987
Reference Sequence Alignment

ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACGGCACTTCGTCACCTGGACCTG
AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAAGACTGTGAGAAG
GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
AAAGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC32
Symbol Nomenclature : LRRC32
SYNONYM : D11S833E; GARP
Designation : garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32
Full Nomenclature : leucine rich repeat containing 32
GENEID : 2615
HGNC : 4161
MIM : 137207
Vega : OTTHUMG00000133687
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00442905        ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
NM_005512.2         ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
NM_001128922.1      ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
                    ************************************************************

HsCD00442905        CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
NM_005512.2         CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
NM_001128922.1      CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
                    ************************************************************

HsCD00442905        CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
NM_005512.2         CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
NM_001128922.1      CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
                    ************************************************************

HsCD00442905        CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACGGCACTTCGTCACCTGGACCTG
NM_005512.2         CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACAGCACTTCGTCACCTGGACCTG
NM_001128922.1      CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACAGCACTTCGTCACCTGGACCTG
                    **************************************.*********************

HsCD00442905        AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
NM_005512.2         AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
NM_001128922.1      AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
                    ************************************************************

HsCD00442905        CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
NM_005512.2         CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
NM_001128922.1      CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
                    ************************************************************

HsCD00442905        GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
NM_005512.2         GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
NM_001128922.1      GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
                    ************************************************************

HsCD00442905        CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
NM_005512.2         CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
NM_001128922.1      CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
                    ************************************************************

HsCD00442905        CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
NM_005512.2         CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
NM_001128922.1      CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
                    ************************************************************

HsCD00442905        CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
NM_005512.2         CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
NM_001128922.1      CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
                    ************************************************************

HsCD00442905        CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
NM_005512.2         CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
NM_001128922.1      CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
                    ************************************************************

HsCD00442905        CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
NM_005512.2         CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
NM_001128922.1      CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
                    ************************************************************

HsCD00442905        GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
NM_005512.2         GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
NM_001128922.1      GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
                    ************************************************************

HsCD00442905        CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
NM_005512.2         CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
NM_001128922.1      CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
                    ************************************************************

HsCD00442905        ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
NM_005512.2         ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
NM_001128922.1      ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
                    ************************************************************

HsCD00442905        CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
NM_005512.2         CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
NM_001128922.1      CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
                    ************************************************************

HsCD00442905        GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
NM_005512.2         GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
NM_001128922.1      GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
                    ************************************************************

HsCD00442905        CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
NM_005512.2         CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
NM_001128922.1      CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
                    ************************************************************

HsCD00442905        TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
NM_005512.2         TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
NM_001128922.1      TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
                    ************************************************************

HsCD00442905        GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
NM_005512.2         GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
NM_001128922.1      GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
                    ************************************************************

HsCD00442905        CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
NM_005512.2         CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
NM_001128922.1      CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
                    ************************************************************

HsCD00442905        CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
NM_005512.2         CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
NM_001128922.1      CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
                    ************************************************************

HsCD00442905        GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
NM_005512.2         GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
NM_001128922.1      GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
                    ************************************************************

HsCD00442905        GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
NM_005512.2         GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
NM_001128922.1      GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
                    ************************************************************

HsCD00442905        GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
NM_005512.2         GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
NM_001128922.1      GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
                    ************************************************************

HsCD00442905        AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
NM_005512.2         AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
NM_001128922.1      AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
                    ************************************************************

HsCD00442905        CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
NM_005512.2         CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
NM_001128922.1      CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
                    ************************************************************

HsCD00442905        CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
NM_005512.2         CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
NM_001128922.1      CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
                    ************************************************************

HsCD00442905        ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
NM_005512.2         ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
NM_001128922.1      ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
                    ************************************************************

HsCD00442905        CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
NM_005512.2         CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
NM_001128922.1      CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
                    ************************************************************

HsCD00442905        CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAAGACTGTGAGAAG
NM_005512.2         CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAG
NM_001128922.1      CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAG
                    ***********************************************.************

HsCD00442905        GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
NM_005512.2         GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
NM_001128922.1      GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
                    ************************************************************

HsCD00442905        CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
NM_005512.2         CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
NM_001128922.1      CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
                    ************************************************************

HsCD00442905        AAAGCC---
NM_005512.2         AAAGCCTAA
NM_001128922.1      AAAGCCTAA
                    ******   

Clone

Original Clone ID : ccsbBroad304_06255

Organism


TAX_ID : 9606
Species Specific ID: 2615

Genome

Chromosome : 11
Map Location : 11q13.5-q14
Ensembl : ENSG00000137507

Reagents

Labome : GARP-antibody