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OPN4 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN4
Gene Name:  opsin 4
Sequence             
Original Clone ID: ccsbBroad304_09368
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443112

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1435nts         Open reading frame : 1 to 1435
Reference Sequence Alignment

ATGAACCCTCCTTCGGGGCCAAGAGTCCTGCCCAGCCCAACCCAAGAGCCCAGCTGCATG
GCCACCCCAGCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGC
CGGCTTCCATCCATCAGTCCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTC
CCCACGGTTGATGTTCCAGACCATGCCCACTATACCCTGGGCACAGTGATCTTGCTGGTG
GGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATACCTTCTGCAGGAGCAGAAGC
CTCCGGACACCTGCCAACATGTTCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCC
TTCACCCAGGCCCCTGTCTTCTTCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAG
ACAGGCTGCGAGTTCTATGCCTTCTGTGGAGCTCTCTTTGGCATTTCCTCCATGATCACC
CTGACGGCCATCGCCCTGGACCGCTACCTGGTAATCACACGCCCGCTGGCCACCTTTGGT
GTGGCGTCCAAGAGGCGTGCGGCATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTGGCC
TGGAGTCTGCCACCCTTCTTCGGCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCC
TGCTCCTGGGACTACATGAGCTTCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGC
TGCTTCGTGTTCTTCCTCCCTCTGCTTATCATCATCTACTGCTACATCTTCATCTTCAGG
GCCATCCGGGAGACAGGACGGGCTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAG
TCCCTGTGGCAGCGGCAGCGGCTGCAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTG
GTCATCCTCCTCTTCGTGCTCTCCTGGGCTCCCTATTCCGCTGTGGCCCTGGTGGCCTTT
GCTGGGTACGCACACGTCCTGACACCCTACATGAGCTCGGTGCCAGCCGTCATCGCCAAG
GCCTCTGCAATCCACAACCCCATCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCC
ATTGCCCAGCACCTGCCCTGCCTGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGC
CCCTACCCCAGCTACCGCTCCACCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTC
AGCTGGATCTCCATACGGAGGCGCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGG
ACACACATGGAGGCAGCAGCTGTGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTC
TACGGTCAGGGTCTGGAGGACTTGGAAGCCAAGGCACCCCCCAGACCCCAGGGACACGAA
GCAGAGACTCCAGGGAAGACCAAGGGGCTGATCCCCAGCCAGGACCCCAGGATG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN4
Symbol Nomenclature : OPN4
SYNONYM : MOP
Designation : melanopsin|opsin-4
Full Nomenclature : opsin 4
GENEID : 94233
HGNC : 14449
MIM : 606665
Vega : OTTHUMG00000018654
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00443112        ATGAACCCTCCTTCGGGGCCAAGAGTCCTGCCCAGCCCAACCCAAGAGCCCAGCTGCATG
NM_001030015.2      ATGAACCCTCCTTCGGGGCCAAGAGTCCCGCCCAGCCCAACCCAAGAGCCCAGCTGCATG
NM_033282.3         ATGAACCCTCCTTCGGGGCCAAGAGTCCCGCCCAGCCCAACCCAAGAGCCCAGCTGCATG
                    **************************** *******************************

HsCD00443112        GCCACCCCAGCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGC
NM_001030015.2      GCCACCCCAGCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGC
NM_033282.3         GCCACCCCAGCACCACCCAGCTGGTGGGACAGCTCCCAGAGCAGCATCTCCAGCCTGGGC
                    ************************************************************

HsCD00443112        CGGCTTCCATCCATCAGTCCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTC
NM_001030015.2      CGGCTTCCATCCATCAGTCCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTC
NM_033282.3         CGGCTTCCATCCATCAGTCCCACAGCACCTGGGACTTGGGCTGCTGCCTGGGTCCCCCTC
                    ************************************************************

HsCD00443112        CCCACGGTTGATGTTCCAGACCATGCCCACTATACCCTGGGCACAGTGATCTTGCTGGTG
NM_001030015.2      CCCACGGTTGATGTTCCAGACCATGCCCACTATACCCTGGGCACAGTGATCTTGCTGGTG
NM_033282.3         CCCACGGTTGATGTTCCAGACCATGCCCACTATACCCTGGGCACAGTGATCTTGCTGGTG
                    ************************************************************

HsCD00443112        GGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATACCTTCTGCAGG---------
NM_001030015.2      GGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATACCTTCT-----GCAGAGCTG
NM_033282.3         GGACTCACGGGGATGCTGGGCAACCTGACGGTCATCTATACCTTCT-----GCAGG----
                    **********************************************              

HsCD00443112        -----------------------------AGCAGAAGCCTCCGGACACCTGCCAACATGT
NM_001030015.2      TGCTTCGTGGAGTCACTGTGATGATGCAGAGCAGAAGCCTCCGGACACCTGCCAACATGT
NM_033282.3         -----------------------------AGCAGAAGCCTCCGGACACCTGCCAACATGT
                                                 *******************************

HsCD00443112        TCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTCT
NM_001030015.2      TCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTCT
NM_033282.3         TCATTATCAACCTCGCGGTCAGCGACTTCCTCATGTCCTTCACCCAGGCCCCTGTCTTCT
                    ************************************************************

HsCD00443112        TCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCT
NM_001030015.2      TCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCT
NM_033282.3         TCACCAGTAGCCTCTATAAGCAGTGGCTCTTTGGGGAGACAGGCTGCGAGTTCTATGCCT
                    ************************************************************

HsCD00443112        TCTGTGGAGCTCTCTTTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACC
NM_001030015.2      TCTGTGGAGCTCTCTTTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACC
NM_033282.3         TCTGTGGAGCTCTCTTTGGCATTTCCTCCATGATCACCCTGACGGCCATCGCCCTGGACC
                    ************************************************************

HsCD00443112        GCTACCTGGTAATCACACGCCCGCTGGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGG
NM_001030015.2      GCTACCTGGTAATCACACGCCCGCTGGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGG
NM_033282.3         GCTACCTGGTAATCACACGCCCGCTGGCCACCTTTGGTGTGGCGTCCAAGAGGCGTGCGG
                    ************************************************************

HsCD00443112        CATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTGGCCTGGAGTCTGCCACCCTTCTTCG
NM_001030015.2      CATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTGGCCTGGAGTCTGCCACCCTTCTTCG
NM_033282.3         CATTTGTCCTGCTGGGCGTTTGGCTCTATGCCCTGGCCTGGAGTCTGCCACCCTTCTTCG
                    ************************************************************

HsCD00443112        GCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCCTGCTCCTGGGACTACATGAGCT
NM_001030015.2      GCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCCTGCTCCTGGGACTACATGAGCT
NM_033282.3         GCTGGAGCGCCTACGTGCCCGAGGGGTTGCTGACATCCTGCTCCTGGGACTACATGAGCT
                    ************************************************************

HsCD00443112        TCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGCTGCTTCGTGTTCTTCCTCCCTC
NM_001030015.2      TCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGCTGCTTCGTGTTCTTCCTCCCTC
NM_033282.3         TCACGCCGGCCGTGCGTGCCTACACCATGCTTCTCTGCTGCTTCGTGTTCTTCCTCCCTC
                    ************************************************************

HsCD00443112        TGCTTATCATCATCTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACAGGACGGG
NM_001030015.2      TGCTTATCATCATCTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACAGGACGGG
NM_033282.3         TGCTTATCATCATCTACTGCTACATCTTCATCTTCAGGGCCATCCGGGAGACAGGACGGG
                    ************************************************************

HsCD00443112        CTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGC
NM_001030015.2      CTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGC
NM_033282.3         CTCTCCAGACCTTCGGGGCCTGCAAGGGCAATGGCGAGTCCCTGTGGCAGCGGCAGCGGC
                    ************************************************************

HsCD00443112        TGCAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCT
NM_001030015.2      TGCAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCT
NM_033282.3         TGCAGAGCGAGTGCAAGATGGCCAAGATCATGCTGCTGGTCATCCTCCTCTTCGTGCTCT
                    ************************************************************

HsCD00443112        CCTGGGCTCCCTATTCCGCTGTGGCCCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGA
NM_001030015.2      CCTGGGCTCCCTATTCCGCTGTGGCCCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGA
NM_033282.3         CCTGGGCTCCCTATTCCGCTGTGGCCCTGGTGGCCTTTGCTGGGTACGCACACGTCCTGA
                    ************************************************************

HsCD00443112        CACCCTACATGAGCTCGGTGCCAGCCGTCATCGCCAAGGCCTCTGCAATCCACAACCCCA
NM_001030015.2      CACCCTACATGAGCTCGGTGCCAGCCGTCATCGCCAAGGCCTCTGCAATCCACAACCCCA
NM_033282.3         CACCCTACATGAGCTCGGTGCCAGCCGTCATCGCCAAGGCCTCTGCAATCCACAACCCCA
                    ************************************************************

HsCD00443112        TCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCCATTGCCCAGCACCTGCCCTGCC
NM_001030015.2      TCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCCATTGCCCAGCACCTGCCCTGCC
NM_033282.3         TCATTTACGCCATCACCCACCCCAAGTACAGGGTGGCCATTGCCCAGCACCTGCCCTGCC
                    ************************************************************

HsCD00443112        TGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGCCCCTACCCCAGCTACCGCTCCA
NM_001030015.2      TGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGCCCCTACCCCAGCTACCGCTCCA
NM_033282.3         TGGGGGTGCTGCTGGGTGTATCACGCCGGCACAGTCGCCCCTACCCCAGCTACCGCTCCA
                    ************************************************************

HsCD00443112        CCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTCAGCTGGATCTCCATACGGAGGC
NM_001030015.2      CCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTCAGCTGGATCTCCATACGGAGGC
NM_033282.3         CCCACCGCTCCACGCTGACCAGCCACACCTCCAACCTCAGCTGGATCTCCATACGGAGGC
                    ************************************************************

HsCD00443112        GCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCAGCTG
NM_001030015.2      GCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCAGCTG
NM_033282.3         GCCAGGAGTCCCTGGGCTCGGAGAGTGAGGTGGGCTGGACACACATGGAGGCAGCAGCTG
                    ************************************************************

HsCD00443112        TGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACT
NM_001030015.2      TGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACT
NM_033282.3         TGTGGGGAGCTGCCCAGCAAGCAAATGGGCGGTCCCTCTACGGTCAGGGTCTGGAGGACT
                    ************************************************************

HsCD00443112        TGGAAGCCAAGGCACCCCCCAGACCCCAGGGACACGAAGCAGAGACTCCAGGGAAGACCA
NM_001030015.2      TGGAAGCCAAGGCACCCCCCAGACCCCAGGGACACGAAGCAGAGACTCCAGGGAAGACCA
NM_033282.3         TGGAAGCCAAGGCACCCCCCAGACCCCAGGGACACGAAGCAGAGACTCCAGGGAAGACCA
                    ************************************************************

HsCD00443112        AGGGGCTGATCCCCAGCCAGGACCCCAGGATG---
NM_001030015.2      AGGGGCTGATCCCCAGCCAGGACCCCAGGATGTAG
NM_033282.3         AGGGGCTGATCCCCAGCCAGGACCCCAGGATGTAG
                    ********************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 05977

Clone

Original Clone ID : ccsbBroad304_09368

Organism


TAX_ID : 9606
Species Specific ID: 94233

Genome

Chromosome : 10
Map Location : 10q22
Ensembl : ENSG00000122375

Reagents

Labome : OPN4-antibody