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MTRF1L (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MTRF1L
Gene Name:  mitochondrial translational release factor 1-like
Sequence             
Original Clone ID: ccsbBroad304_03429
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00443756

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 814nts         Open reading frame : 1 to 814
Reference Sequence Alignment

ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
GTCCGGATAGTTCATCTTCCAACAGATTGGAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MTRF1L
Symbol Nomenclature : MTRF1L
SYNONYM : HMRF1L; MRF1L; mtRF1a
Designation : mitochondrial release factor 1 like|peptide chain release factor 1-like, mitochondrial
Full Nomenclature : mitochondrial translational release factor 1-like
GENEID : 54516
Locus Tag : RP1-101K10.1
GenBank Accession : HQ447880
HGNC : 21051
MIM : 613542
Vega : OTTHUMG00000015857
Target GenBank: HQ447880

 

Reference Sequence Alignment

  

HsCD00443756        ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
NM_001114184.1      ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
NM_019041.5         ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
                    ************************************************************

HsCD00443756        CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
NM_001114184.1      CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
NM_019041.5         CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
                    ************************************************************

HsCD00443756        GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
NM_001114184.1      GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
NM_019041.5         GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
                    ************************************************************

HsCD00443756        AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
NM_001114184.1      AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
NM_019041.5         AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
                    ************************************************************

HsCD00443756        ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
NM_001114184.1      ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
NM_019041.5         ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
                    ************************************************************

HsCD00443756        ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
NM_001114184.1      ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
NM_019041.5         ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
                    ************************************************************

HsCD00443756        TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
NM_001114184.1      TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
NM_019041.5         TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
                    ************************************************************

HsCD00443756        GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
NM_001114184.1      GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
NM_019041.5         GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
                    ************************************************************

HsCD00443756        AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
NM_001114184.1      AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
NM_019041.5         AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
                    ************************************************************

HsCD00443756        TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
NM_001114184.1      TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
NM_019041.5         TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
                    ************************************************************

HsCD00443756        AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
NM_001114184.1      AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
NM_019041.5         AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
                    ************************************************************

HsCD00443756        GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
NM_001114184.1      GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
NM_019041.5         GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
                    ************************************************************

HsCD00443756        ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
NM_001114184.1      ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
NM_019041.5         ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
                    ************************************************************

HsCD00443756        GTCCGGATAGTTCATCTTCCAACAGATTGGAAG---------------------------
NM_001114184.1      GTCCGGATAGTTCATCTTCCAACAGATTGGAAGTAA------------------------
NM_019041.5         GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
                    *************************.*   .:                            

HsCD00443756        ------------------------------------------------------------
NM_001114184.1      ------------------------------------------------------------
NM_019041.5         CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
                                                                                

HsCD00443756        ------------------------------------------------------------
NM_001114184.1      ------------------------------------------------------------
NM_019041.5         GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
                                                                                

HsCD00443756        ------------------------------------------------------------
NM_001114184.1      ------------------------------------------------------------
NM_019041.5         TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
                                                                                

HsCD00443756        ------------------------------------------------------------
NM_001114184.1      ------------------------------------------------------------
NM_019041.5         AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
                                                                                

HsCD00443756        ------------------------------------------------------------
NM_001114184.1      ------------------------------------------------------------
NM_019041.5         CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT
                                                                                

HsCD00443756        ---
NM_001114184.1      ---
NM_019041.5         TAA
                       

Protein

SMART domain : PCRF :
HPRD : 17613

Clone

Original Clone ID : ccsbBroad304_03429

Organism


TAX_ID : 9606
Species Specific ID: 54516

Genome

Chromosome : 6
Map Location : 6q25-q26
Ensembl : ENSG00000112031

Reagents

Labome : MTRF1L-antibody