Gene:
Gene Symbol:
RB1CC1
Gene Name:
RB1-inducible coiled-coil 1
Original Clone ID:
FLH186014.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00044465
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGTTATATGTATTTCTGGTTAACACTGGAACTACTCTAACATTTGACACTGAACTT
ACAGTGCAAACTGTGGCAGACCTTAAGCATGCCATTCAAAGCAAATACAAGATTGCTATT
CAACACCAGGTGCTGGTGGTCAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTGT
ACCTACAGTGCTGGGACGGATACAAATCCAATTTTTCTTTTTAACAAAGAAATGATCTTA
TGTGATCGTCCACCTGCTATTCCTAAAACTACCTTTTCGACAGAAAATGACATGGAAATA
AAAGTTGAAGAATCTCTTATGATGCCTGCAGTTTTTCATACTGTTGCTTCAAGGACACAG
CTTGCATTGGAAATGTATGAAGTTGCCAAGAAACTTTGTTCTTTTTGTGAAGGTCTTGTA
CATGATGAACATCTTCAACACCAAGGCTGGGCTGCAATCATGGCCAACCTGGAGGACTGT
TCAAATTCATACCAAAAGCTACTTTTCAAGTTTGAAAGTATTTATTCAAATTATCTGCAG
TCCATAGAAGACATCAAGTTAAAACTTACTCATTTAGGAACTGCAGTTTCAGTAATGGCC
AAGATTCCACTGTTGGAGTGCCTAACCAGACATAGTTACAGAGAATGTTTGGGAAGACTG
GATTCTTTACCTGAACATGAAGACTCAGAAAAAGCTGAGACGAAAAGATCCACTGAACTG
GTGCTCTCTCCTGATATGCCTAGAACAACTAACGAATCTTTGTTAACCTCATTTCCCAAG
TCAGTGGAACATGTGTCCCCAGATACCGCAGATGCTGAAAGTGGCAAAGAAATTAGGGAA
TCTTGTCAAAGTACTGTTCATCAGCAAGATGAAACTACGATTGACACTAAAGATGGTGAT
CTGCCCTTTTTTAATGTCTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGAT
GTGGAATCTTTGGTCAGGAAGTGCTTTGATTCTATGAGCAGGCTTGATCCAAGGATTATT
CGACCATTTATAGCAGAATGCCGTCAAACTATTGCCAAACTTGATAATCAGAATATGAAA
GCCATTAAAGGACTTGAAGATCGGCTCTACGCCCTGGACCAGATGATTGCTAGCTGTGGC
CGACTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAAGAGAGCT
GAAAACTTAAAGGATGCATCTGTATTACCTGATTTATGCCTGAGTCACGCAAATCAGTTG
ATGATTATGTTGCAAAATCATAGAAAACTGTTAGATATTAAGCAGAAGTGTACCACTGCC
AAACAAGAACTAGCAAATAACCTACATGTCAGACTGAAGTGGTGTTGCTTTGTAATGCTT
CATGCTGATCAAGATGGAGAGAAGTTACAAGCTTTGCTCCGCCTCGTAATAGAGCTGTTA
GAAAGAGTCAAAATTGTTGAAGCTCTTAGTACAGTTCCTCAGATGTACTGCTTAGCTGTT
GTTGAGGTTGTAAGAAGAAAAATGTTCATAAAACACTACAGGGAGTGGGCTGGTGCTTTA
GTCAAAGATGGAAAGAGATTATATGAAGCAGAAAAATCAAAAAGGGAATCCTTTGGGAAA
TTATTTAGGAAGTCTTTTTTAAGAAATCGTCTGTTTAGGGGACTGGACTCCTGGCCCCCT
TCCTTTTGTACTCAAAAGCCTCGAAAGTTTGACTGTGAACTTCCAGATATTTCATTAAAA
GATTTACAGTTTCTGCAATCATTTTGTCCTTCGGAAGTTCAGCCATTCCTCAGGGTTCCC
TTACTTTGTGACTTTGAACCTCTACACCAGCATGTACTTGCTCTACATAATTTGGTAAAA
GCAGCACAAAGTTTGGATGAAATGTCACAGACCATTACAGATCTACTGAGTGAACAAAAG
GCATCTGTGAGTCAGACATCCCCACAGTCTGCTTCTTCACCAAGGATGGAAAGTACAGCA
GGAATTACAACTACTACCTCACCGAGAACTCCTCCACCACTGACTGTTCAGGATCCCTTA
TGTCCTGCAGTTTGTCCCTTAGAAGAATTATCTCCAGATAGTATTGATGCACATACGTTT
GATTTTGAAACTATTCCCCATCCAAACATAGAACAGACTATTCACCAAGTTTCTTTAGAC
TTGGATTCATTAGCAGAAAGTCCTGAATCAGATTTTATGTCTGCTGTGAATGAGTTTGTA
ATAGAAGAAAATTTGTCGTCTCCTAATCCTATAAGTGATCCACAAAGCCCAGAAATGATG
GTGGAATCACTTTATTCATCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACA
AATGTATGTGGTAAGGAGGATTTTGGAGATCATACTTCTCTGAATGTCCAGTTGGAAAGA
TGTAGAGTTGTTGCCCAAGACTCTCACTTCAGTATACAAACCATTAAGGAAGACCTTTGC
CACTTTAGAACATTTGTACAAAAAGAACAGTGTGACTTCTCAAATTCATTAAAATGTACA
GCAGTAGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAATAACACTAAAA
GAAAAACATCAAAAAGAACTACTGTCTTTAAAAAATGAATATGAAGGTAAACTTGACGGA
CTAATAAAGGAAACTGAAGAGAATGAAAACAAAATTAAAAAATTGAAGGGAGAGTTAGTA
TGCCTTGAGGAGGTTTTACAAAATAAAGATAATGAATTTGCTTTGGTTAAACATGAAAAA
GAAGCTGTAATCTGCCTGCAGAATGAAAAGGATCAGAAGTTGTTAGAGATGGAAAATATA
ATGCACTCTCAAAATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATAGTGTTAGAA
GACTTAAAAAAGCTCCATGTTGAAAATGATGAGAAGTTACAGTTATTGAGGGCAGAACTT
CAGTCCTTGGAGCAAAGTCATCTAAAGGAATTAGAGGACACACTTCAGGTTAGGCACATA
CAAGAGTTTGAGAAGGTTATGACAGACCACAGAGTTTCTTTGGAGGAATTAAAAAAGGAA
AACCAACAAATAATTAATCAAATACAAGAATCTCATGCTGAAATTATCCAGGAAAAAGAA
AAACAGTTACAGGAATTAAAACTCAAGGTTTCTGATTTGTCAGACACGAGATGCAAGTTA
GAGGTTGAACTTGCGTTGAAGGAAGCAGAAACTGATGAAATAAAAATTTTGCTGGAAGAA
AGCAGAGCCCAGCAGAAGGAGACCTTGAAATCTCTTCTTGAACAAGAGACAGAAAATTTG
AGAACAGAAATTAGTAAACTCAACCAAAAGATTCAGGATAATAATGAAAATTATCAGGTG
GGCTTAGCAGAGCTAAGAACTTTAATGACAATTGAAAAAGATCAGTGTATTTCCGAGTTA
ATTAGTAGACATGAAGAAGAATCTAATATACTTAAAGCTGAATTAAACAAAGTAACATCT
TTGCATAACCAAGCATTTGAAATAGAAAAAAACCTAAAAGAACAAATAATTGAACTGCAG
AGTAAATTGGATTCAGAATTGAGTGCTCTTGAAAGACAAAAAGATGAAAAAATTACCCAA
CAAGAAGAGAAATACGAAGCTATTATCCAGAACCTTGAGAAAGACAGACAAAAATTGGTC
AGCAGCCAGGAGCAAGACAGAGAACAGTTAATTCAGAAGCTTAATTGTGAAAAAGATGAA
GCTATTCAGACTGCCCTAAAAGAATTTAAATTGGAGAGAGAAGTTGTTGAGAAAGAGTTA
TTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCAAAAAGTCCTGCCATTGACTCTACC
AGAGGAGATTCTTCAAGCTTAGTTGCTGAACTTCAAGAAAAGCTTCAGGAAGAAAAAGCT
AAGTTTCTAGAACAACTTGAAGAGCAAGAAAAAAGAAAGAATGAAGAAATGCAAAATGTT
CGAACATCTTTGATTGCGGAACAACAGACCAATTTTAACACTGTTTTAACAAGAGAGAAA
ATGAGAAAAGAAAACATAATAAATGATCTTAGTGATAAGTTGAAAAGTACAATGCAGCAA
CAAGAACGGGATAAAGATTTGATAGAGTCACTTTCTGAAGATCGAGCTCGTTTGCTTGAG
GAAAAGAAAAAGCTTGAAGAAGAAGTCAGTAAGTTGCGCAGTAGCAGTTTTGTTCCTTCA
CCATATGTAGCTACAGCCCCAGAACTTTATGGAGCTTGTGCACCTGAACTCCCAGGTGAA
TCAGATAGATCCGCTGTGGAAACAGCAGATGAAGGAAGAGTGGATTCAGCAATGGAGACA
AGCATGATGTCTGTACAAGAAAATATTCATATGTTGTCTGAAGAAAAACAGCGGATAATG
CTGTTAGAACGAACATTGCAATTGAAAGAAGAAGAAAATAAACGGTTAAATCAAAGACTG
ATGTCTCAGAGCATGTCTTCAGTATCTTCAAGGCATTCTGAAAAGATAGCTATTAGAGAT
TTTCAGGTGGGAGATTTGGTACTCATCATCCTAGACGAACGCCATGACAATTATGTGTTA
TTTACTGTTAGTCCTACTTTATATTTTCTACATTCAGAGTCTCTACCTGCCCTGGATCTC
AAACCAGGTGAGGGTGCTTCAGGTGCATCTAGAAGACCCTGGGTACTCGGAAAAGTAATG
GAAAAAGAATACTGTCAAGCCAAAAAGGCACAAAACAGATTTAAAGTTCCTTTGGGGACA
AAGTTTTACAGAGTGAAAGCCGTATCATGGAATAAGAAAGTA
Protein sequence predicted based on nucleotide sequence
MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVC TYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQ LALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQ SIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAETKRSTEL VLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDTKDGD LPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAECRQTIAKLDNQNMK AIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKRAENLKDASVLPDLCLSHANQL MIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELL ERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGK LFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVP LLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQTSPQSASSPRMESTA GITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSIDAHTFDFETIPHPNIEQTIHQVSLD LDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINAIDSRRMQDT NVCGKEDFGDHTSLNVQLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCT AVEIRNIIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLKGELV CLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLE DLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQVRHIQEFEKVMTDHRVSLEELKKE NQQIINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEE SRAQQKETLKSLLEQETENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISEL ISRHEEESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDEKITQ QEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTALKEFKLEREVVEKEL LEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNV RTSLIAEQQTNFNTVLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLE EKKKLEEEVSKLRSSSFVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMET SMMSVQENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRD FQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVM EKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
123
End on reference sequence
4907
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
