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KLHDC7B (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHDC7B
Gene Name:  kelch domain containing 7B
Sequence             
Original Clone ID: ccsbBroad304_13026
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00444967

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1486nts         Open reading frame : 1 to 1486
Reference Sequence Alignment

ATGCCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCC
TCTGCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCC
CCGAGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCG
GGGGAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCC
CGGAAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGG
AAGCCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGAC
CTGGGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCG
CAGGAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTC
TACCGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGC
CGGGCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTC
CCCAGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCT
GCGCACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCC
GAGGAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCG
GGGGGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAAC
CCTCTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAG
CTGGTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAG
TGCTACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTC
CCTGTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTC
TTCTACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGT
GCCAGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGAC
CTGCTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGC
AGGGCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAAC
ACCATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCC
CAGTTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCA
TTCATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHDC7B
Symbol Nomenclature : KLHDC7B
Designation : kelch domain-containing protein 7B
Full Nomenclature : kelch domain containing 7B
GENEID : 113730
HGNC : 25145
Vega : OTTHUMG00000150250
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00444967      ------------------------------------------------------------
NM_138433.3       ATGGTTCTTAGAAGCCACCCCTTCCCCAGGCAAGACAGGCCCCAAGGGAGTGTCCCGAGG
                                                                              

HsCD00444967      ------------------------------------------------------------
NM_138433.3       GCGGTTCCCGGGAGCCCCGTGGGTCCCAGCACTTCCACACACTCTGAGGACAGACACGGC
                                                                              

HsCD00444967      ------------------------------------------------------------
NM_138433.3       CCCTCTTCTTCAGTGGGGACAGTCATAGGGACAGGTACAGGGGGCCTGGTTGAGGCTGGA
                                                                              

HsCD00444967      ------------------------------------------------------------
NM_138433.3       GGTCAGCCACAGCCAAGAAGCTCCGAGACCAACGGATCGCCCAGCCCAGACCCTCCCCCA
                                                                              

HsCD00444967      ---------------------------------------------------------ATG
NM_138433.3       GGCCTAAGAGGAGAGGGAACCAGGGAGAAAAGTCTAGACCCGCTGCCCCAAGCCGCGATG
                                                                           ***

HsCD00444967      CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
NM_138433.3       CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
                  ************************************************************

HsCD00444967      GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
NM_138433.3       GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
                  ************************************************************

HsCD00444967      AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
NM_138433.3       AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
                  ************************************************************

HsCD00444967      GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
NM_138433.3       GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
                  ************************************************************

HsCD00444967      AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
NM_138433.3       AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
                  ************************************************************

HsCD00444967      CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
NM_138433.3       CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
                  ************************************************************

HsCD00444967      GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
NM_138433.3       GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
                  ************************************************************

HsCD00444967      GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
NM_138433.3       GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
                  ************************************************************

HsCD00444967      CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
NM_138433.3       CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
                  ************************************************************

HsCD00444967      GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
NM_138433.3       GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
                  ************************************************************

HsCD00444967      AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
NM_138433.3       AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
                  ************************************************************

HsCD00444967      CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
NM_138433.3       CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
                  ************************************************************

HsCD00444967      GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
NM_138433.3       GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
                  ************************************************************

HsCD00444967      GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
NM_138433.3       GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
                  ************************************************************

HsCD00444967      CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
NM_138433.3       CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
                  ************************************************************

HsCD00444967      GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
NM_138433.3       GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
                  ************************************************************

HsCD00444967      TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
NM_138433.3       TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
                  ************************************************************

HsCD00444967      GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
NM_138433.3       GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
                  ************************************************************

HsCD00444967      TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
NM_138433.3       TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
                  ************************************************************

HsCD00444967      AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
NM_138433.3       AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
                  ************************************************************

HsCD00444967      CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
NM_138433.3       CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
                  ************************************************************

HsCD00444967      GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
NM_138433.3       GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
                  ************************************************************

HsCD00444967      ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
NM_138433.3       ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
                  ************************************************************

HsCD00444967      TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
NM_138433.3       TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
                  ************************************************************

HsCD00444967      ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC---
NM_138433.3       ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTCTGA
                  ******************************************   

Protein

SMART domain : Kelch :
HPRD : 14453

Clone

Original Clone ID : ccsbBroad304_13026

Organism


TAX_ID : 9606
Species Specific ID: 113730

Genome

Chromosome : 22
Map Location : 22q13.33
Ensembl : ENSG00000130487

Reagents

Labome : KLHDC7B-antibody