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ADAL (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ADAL
Gene Name:  adenosine deaminase-like
Sequence             
Original Clone ID: ccsbBroad304_14418
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446071

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 803nts         Open reading frame : 1 to 803
Reference Sequence Alignment

ATGATAGAGGCAGAAGAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAA
GTGGAACTTCATGCCCACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATA
GCCCAGAAGCCAGATCTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAA
AGAACTTTGGAAGAATGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCT
GAAGATATTCTAATGGTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAG
TACCTGGAACTAAGGAGCACACCCAGAAGAGAAAATGCTACCGGAATGACTAAAAAGACT
TATGTGGAATCTATACTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGAT
GTTAGGTATTTGATAGCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTA
AAACTTGCCGAGGAGTTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGT
GGAGACCCTACTGTAGGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAA
GCAGGTCTGAAGTTAGCATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAAGAAACACA
AATACTCCTGGATCTGCTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGA
GGGAGGATCCCTGGATCTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGC
TTGGAGTTTCAGGTCTTCCAGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
 early stop codon; insertion or deletion in coding sequence
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ADAL
Symbol Nomenclature : ADAL
Designation : adenosine deaminase-like protein
Full Nomenclature : adenosine deaminase-like
GENEID : 161823
HGNC : 31853
Vega : OTTHUMG00000176646
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00446071        ATGATAGAGGCAGAAGAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAA
NM_001012969.3      ATGATAGAGGCAGAAGAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAA
NM_001159280.2      ATGATAGAGGCAGAAGAGCAACAGCCTTGCAAGACAGACTTCTATTCTGAATTGCCAAAA
                    ************************************************************

HsCD00446071        GTGGAACTTCATGCCCACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATA
NM_001012969.3      GTGGAACTTCATGCCCACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATA
NM_001159280.2      GTGGAACTTCATGCCCACTTGAATGGATCCATTAGTTCTCATACCATGAAGAAATTAATA
                    ************************************************************

HsCD00446071        GCCCAGAAGCCAGATCTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAA
NM_001012969.3      GCCCAGAAGCCAGATCTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAA
NM_001159280.2      GCCCAGAAGCCAGATCTTAAAATCCACGATCAGATGACTGTGATTGACAAGGGAAAGAAA
                    ************************************************************

HsCD00446071        AGAACTTTGGAAGAATGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCT
NM_001012969.3      AGAACTTTGGAAGAATGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCT
NM_001159280.2      AGAACTTTGGAAGAATGTTTCCAGATGTTTCAAACTATTCATCAGCTTACTAGTAGCCCT
                    ************************************************************

HsCD00446071        GAAGATATTCTAATGGTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAG
NM_001012969.3      GAAGATATTCTAATGGTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAG
NM_001159280.2      GAAGATATTCTAATGGTCACAAAAGATGTCATAAAAGAATTTGCAGATGACGGCGTCAAG
                    ************************************************************

HsCD00446071        TACCTGGAACTAAGGAGCACACCCAGAAGAGAAAATGCTACCGGAATGACTAAAAAGACT
NM_001012969.3      TACCTGGAACTAAGGAGCACACCCAGAAGAGAAAATGCTACTGGAATGACTAAAAAGACT
NM_001159280.2      TACCTGGAACTAAGGAGCACACCCAGAAGAGAAAATGCTACTGGAATGACTAAAAAGACT
                    ***************************************** ******************

HsCD00446071        TATGTGGAATCTATACTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGAT
NM_001012969.3      TATGTGGAATCTATACTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGAT
NM_001159280.2      TATGTGGAATCTATACTTGAAGGTATAAAACAGTCCAAACAAGAAAACTTGGACATTGAT
                    ************************************************************

HsCD00446071        GTTAGGTATTTGATAGCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTA
NM_001012969.3      GTTAGGTATTTGATAGCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTA
NM_001159280.2      GTTAGGTATTTGATAGCAGTTGACAGAAGAGGTGGCCCTTTAGTAGCCAAGGAGACTGTA
                    ************************************************************

HsCD00446071        AAACTTGCCGAGGAGTTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGT
NM_001012969.3      AAACTTGCCGAGGAGTTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGT
NM_001159280.2      AAACTTGCCGAGGAGTTCTTCCTTTCTACTGAGGGTACAGTTCTTGGCCTTGACCTCAGT
                    ************************************************************

HsCD00446071        GGAGACCCTACTGTAGGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAA
NM_001012969.3      GGAGACCCTACTGTAGGACAAGCAAAAGACTTCTTGGAACCTCTTTTAGAAGCTAAGAAA
NM_001159280.2      GGAGACCCTACTATTCCAAACCAAAAAAAAGAAACACAAATACTCCTGGATCTGCTTCCT
                    ************.*:  *.*. .****.*. :.: . **. :**  *.**:   .: ..:

HsCD00446071        GCAGGTCTGAAGTTAG--------------------------------------------
NM_001012969.3      GCAGGTCTGAAGTTAG--------------------------------------------
NM_001159280.2      GACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCTGGATCTGGTG
                    *...*:.* ..* ::*                                            

HsCD00446071        ------------------------------------------------------------
NM_001012969.3      ------------------------------------------------------------
NM_001159280.2      GACTTTGTGAGGCAACATCGGATACCACTGGAACTCTGTTTGACCTCAAACGTCAAAAGT
                                                                                

HsCD00446071        ----CATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAAGAAACACAAATACTCCTGGA
NM_001012969.3      ----CATTGCATCTTTCAGAGATTCCAAACCAAAAAAAAG-AAACACAAATACTCCTGGA
NM_001159280.2      CAGACAGTTCCATCTTATGACCAGCACCATTTCGGATTCTGGTACAGCATTGCCCATCCT
                        ** * *.:  **.:** .: *...*  :...*::.  .:*** .*:*.* *.*  :

HsCD00446071        TCTGCTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCT
NM_001012969.3      TCTGCTTCCTGACAGAATCGGGCATGGAACATTTCTCAACTCCGGTGAGGGAGGATCCCT
NM_001159280.2      TCTGTGATCTGTACTGATGATAAGGGTGTTTTTGCAACACACCTTTCTCAAGAGTACCAG
                    ****  : ***:.. .** . ... * .: :** *:..**:**  * : ....*::**. 

HsCD00446071        GGATCTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGCTTGGAGTTTCAG
NM_001012969.3      GGATCTGGTGGACTTTGTGAGGCAACATCGGATACCACTGGGTAAGGCTTGGAGTTTCAG
NM_001159280.2      CTGGCAGCTGAAACATTTAATTTGACCCAGTCTCAGGTGTGGGATCTGTCTTATGAATCC
                      . *:* **.*. :* *.*   .**. .* .*.. .   ** *:   *   *  :: . 

HsCD00446071        GTCTTCCAGA--------------------------------------------------
NM_001012969.3      GTCTTCCAGATGA-----------------------------------------------
NM_001159280.2      ATCAACTACATCTTTGCTTCTGACAGCACCAGATCTGAACTGAGGAAGAAATGGAATCAC
                    .**::* * *                                                  

HsCD00446071        ---------------------------
NM_001012969.3      ---------------------------
NM_001159280.2      CTGAAGCCCAGAGTGTTACATATTTAA
                                               

Pathways

Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Purine metabolism
Reactome : Purine salvage

Protein

HPRD : 11779

Clone

Original Clone ID : ccsbBroad304_14418

Organism


TAX_ID : 9606
Species Specific ID: 161823

Genome

Chromosome : 15
Map Location : 15q15.3
Ensembl : ENSG00000168803

Reagents

Labome : ADAL-antibody