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KBTBD3 (Homo sapiens) in pLX304 (Gateway V5-tagged lentiviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD3
Gene Name:  kelch repeat and BTB (POZ) domain containing 3
Sequence             
Original Clone ID: ccsbBroad304_04965
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pLX304               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00446537

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1837nts         Open reading frame : 1 to 1837
Reference Sequence Alignment

ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
AAATACAGAGACCCATGGTTTTCTAATCTATGTGCT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pLX304

pLX304 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  CMV forward
Reverse:  Unknown
Description: Gateway compatible lentiviral vector with a C-terminal V5 tag and CMV promoter for mammalian cell expression; blasticidin resistance in mammalian cells; ampicillin resistance in bacteria; Gateway cloning.
Comments: None
Size (bp): 9377
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name: David Root
Publications: PMID: 21706014
Title: A public genome-scale lentiviral expression library of human ORFs

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
polypurine tract cPPT human immunodeficiency virus type 1 central polypurine tract 3027 3042
promoter T7 T7 promoter 3690 3672
promoter CMV CMV promoter 14 566
recombination site attR1 595 719
recombination site attR2 2282 2297
selectable marker CAT CAT/CamR Chloramphenical resistance 828 1487
selectable marker ccdB Control of cell death B gene 1829 2134
selectable marker AmpR Ampicillin resistance gene 4444 5304
selectable marker BlastR Blasticidin resistance gene 8753 9148
tag V5 C-terminal V5 tag 2301 2342
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 2386 2961

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD3
Symbol Nomenclature : KBTBD3
SYNONYM : BKLHD3
Designation : BTB and kelch domain containing 3|BTB and kelch domain-containing protein 3|kelch repeat and BTB domain-containing protein 3
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 3
GENEID : 143879
GenBank Accession : EU832015
HGNC : 22934
Vega : OTTHUMG00000166251
Target GenBank: EU832015

 

Reference Sequence Alignment

  

HsCD00446537      ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
NM_198439.2       ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
NM_152433.3       ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
                  ************************************************************

HsCD00446537      CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
NM_198439.2       CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
NM_152433.3       CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
                  ************************************************************

HsCD00446537      GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
NM_198439.2       GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
NM_152433.3       GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
                  ************************************************************

HsCD00446537      GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
NM_198439.2       GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
NM_152433.3       GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
                  ************************************************************

HsCD00446537      TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
NM_198439.2       TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
NM_152433.3       TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
                  ************************************************************

HsCD00446537      AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
NM_198439.2       AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
NM_152433.3       AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
                  ************************************************************

HsCD00446537      AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
NM_198439.2       AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
NM_152433.3       AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
                  ************************************************************

HsCD00446537      TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
NM_198439.2       TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
NM_152433.3       TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
                  ************************************************************

HsCD00446537      TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
NM_198439.2       TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
NM_152433.3       TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
                  ************************************************************

HsCD00446537      TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
NM_198439.2       TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
NM_152433.3       TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
                  ************************************************************

HsCD00446537      TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
NM_198439.2       TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
NM_152433.3       TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
                  ************************************************************

HsCD00446537      AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
NM_198439.2       AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
NM_152433.3       AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
                  ************************************************************

HsCD00446537      GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
NM_198439.2       GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
NM_152433.3       GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
                  ************************************************************

HsCD00446537      TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
NM_198439.2       TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
NM_152433.3       TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
                  ************************************************************

HsCD00446537      TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
NM_198439.2       TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
NM_152433.3       TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
                  ************************************************************

HsCD00446537      AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
NM_198439.2       AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
NM_152433.3       AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
                  ************************************************************

HsCD00446537      TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
NM_198439.2       TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
NM_152433.3       TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
                  ************************************************************

HsCD00446537      GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
NM_198439.2       GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
NM_152433.3       GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
                  ************************************************************

HsCD00446537      CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
NM_198439.2       CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
NM_152433.3       CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
                  ************************************************************

HsCD00446537      AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
NM_198439.2       AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
NM_152433.3       AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
                  ************************************************************

HsCD00446537      GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
NM_198439.2       GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
NM_152433.3       GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
                  ************************************************************

HsCD00446537      CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
NM_198439.2       CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
NM_152433.3       CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
                  ************************************************************

HsCD00446537      CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
NM_198439.2       CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
NM_152433.3       CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
                  ************************************************************

HsCD00446537      TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
NM_198439.2       TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
NM_152433.3       TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
                  ************************************************************

HsCD00446537      GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
NM_198439.2       GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
NM_152433.3       GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
                  ************************************************************

HsCD00446537      TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
NM_198439.2       TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
NM_152433.3       TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
                  ************************************************************

HsCD00446537      GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
NM_198439.2       GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
NM_152433.3       GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
                  ************************************************************

HsCD00446537      GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
NM_198439.2       GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
NM_152433.3       GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
                  ************************************************************

HsCD00446537      GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
NM_198439.2       GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
NM_152433.3       GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
                  ************************************************************

HsCD00446537      GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
NM_198439.2       GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
NM_152433.3       GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
                  ************************************************************

HsCD00446537      AAATACAGAGACCCATGGTTTTCTAATCTATGTGCT---
NM_198439.2       AAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTGA
NM_152433.3       AAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTGA
                  ************************************   

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
HPRD : 10005

Clone

Original Clone ID : ccsbBroad304_04965

Organism


TAX_ID : 9606
Species Specific ID: 143879

Genome

Chromosome : 11
Map Location : 11q22.3
Ensembl : ENSG00000182359

Reagents

Labome : KBTBD3-antibody