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Detailed Vector Information: pMCSG53

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG53              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments: None
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00450863

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG53

pMCSG53 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments: 1 kb of sequence (including the rop repressor) removed from pMCSG52 by digestion with PshAI and Tth111I, followed by treatment with Klenow, dNTPs and T4 DNA ligase
Size (bp): 4808
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Argonne National Laboratory
PSI:Biology
Publications: PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 2801 3389
ligation independent cloning LIC Ligation independent cloning site 204 235
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI LacI CDS 1413 2372
selectable marker AmpR ampicillin resistance gene 3560 4420
tRNA argU argU tRNA gene 443 799
tRNA ileX ileX tRNA gene 959 1110
tag His N-terminal 6xHis tag 268 285
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None