DNASU Plasmid Repository • 480.965.5697 | Email

EXOSC8 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  EXOSC8
Gene Name:  exosome component 8
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH194106.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045169

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 831nts         Open reading frame : 1 to 831
Reference Sequence Alignment

ATGGCGGCTGGGTTCAAAACCGTGGAACCTCTGGAGTATTACAGGAGATTTCTGAAAGAG
AACTGCCGTCCTGATGGAAGAGAACTTGGTGAATTCAGAACCACAACTGTCAACATCGGT
TCAATTAGTACCGCAGATGGTTCTGCTTTAGTGAAGTTGGGAAATACTACAGTAATCTGT
GGAGTTAAAGCAGAATTTGCAGCACCATCAACAGATGCCCCTGATAAAGGATACGTTGTT
CCTAATGTGGATCTACCACCCCTGTGTTCATCGAGATTCCGGTCTGGACCTCCTGGAGAA
GAGGCCCAAGTGGCTAGCCAATTCATTGCAGATGTCATTGAAAATTCACAGATAATTCAG
AAAGAGGACTTATGCATTTCTCCAGGAAAGCTTGTCTGGGTTCTATACTGTGATCTCATT
TGCCTCGACTACGATGGAAACATTTTGGATGCCTGCACATTTGCTTTGCTAGCGGCTTTA
AAAAATGTACAGTTGCCTGAAGTTACTATAAATGAAGAAACTGCTTTAGCAGAAGTTAAT
TTAAAGAAGAAAAGTTATTTGAATATTAGAACTCATCCAGTTGCAACTTCCTTTGCTGTG
TTTGATGACACTTTGCTTATAGTTGACCCTACTGGAGAGGAGGAACATCTGGCAACAGGA
ACCTTAACAATAGTAATGGATGAGGAAGGCAAACTCTGTTGTCTTCACAAACCAGGTGGA
AGTGGGCTAACTGGAGCTAAACTTCAGGACTGTATGAGCCGAGCAGTTACAAGACACAAA
GAAGTTAAAAAACTGATGGATGAAGTAATTAAGAGTATGAAACCCAAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 857
Start on reference sequence 27
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EXOSC8
Symbol Nomenclature : EXOSC8
SYNONYM : CIP3; EAP2; OIP2; RP11-421P11.3; RRP43; Rrp43p; bA421P11.3; p9
Designation : CBP-interacting protein 3|OIP-2|Opa interacting protein 2|exosome complex component RRP43|exosome complex exonuclease RRP43|opa-interacting protein 2|ribosomal RNA-processing protein 43
Full Nomenclature : exosome component 8
GENEID : 11340
HGNC : 17035
MIM : 606019
Vega : OTTHUMG00000016742
Target GenBank: BC020773

 

Reference Sequence Alignment

  

HsCD00045169      ATGGCGGCTGGGTTCAAAACCGTGGAACCTCTGGAGTATTACAGGAGATTTCTGAAAGAG
NM_181503.2       ATGGCGGCTGGGTTCAAAACCGTGGAACCTCTGGAGTATTACAGGAGATTTCTGAAAGAG
                  ************************************************************

HsCD00045169      AACTGCCGTCCTGATGGAAGAGAACTTGGTGAATTCAGAACCACAACTGTCAACATCGGT
NM_181503.2       AACTGCCGTCCTGATGGAAGAGAACTTGGTGAATTCAGAACCACAACTGTCAACATCGGT
                  ************************************************************

HsCD00045169      TCAATTAGTACCGCAGATGGTTCTGCTTTAGTGAAGTTGGGAAATACTACAGTAATCTGT
NM_181503.2       TCAATTAGTACCGCAGATGGTTCTGCTTTAGTGAAGTTGGGAAATACTACAGTAATCTGT
                  ************************************************************

HsCD00045169      GGAGTTAAAGCAGAATTTGCAGCACCATCAACAGATGCCCCTGATAAAGGATACGTTGTT
NM_181503.2       GGAGTTAAAGCAGAATTTGCAGCACCATCAACAGATGCCCCTGATAAAGGATACGTTGTT
                  ************************************************************

HsCD00045169      CCTAATGTGGATCTACCACCCCTGTGTTCATCGAGATTCCGGTCTGGACCTCCTGGAGAA
NM_181503.2       CCTAATGTGGATCTACCACCCCTGTGTTCATCGAGATTCCGGTCTGGACCTCCTGGAGAA
                  ************************************************************

HsCD00045169      GAGGCCCAAGTGGCTAGCCAATTCATTGCAGATGTCATTGAAAATTCACAGATAATTCAG
NM_181503.2       GAGGCCCAAGTGGCTAGCCAGTTCATTGCAGATGTCATTGAAAATTCACAGATAATTCAG
                  ********************.***************************************

HsCD00045169      AAAGAGGACTTATGCATTTCTCCAGGAAAGCTTGTCTGGGTTCTATACTGTGATCTCATT
NM_181503.2       AAAGAGGACTTATGCATTTCTCCAGGAAAGCTTGTCTGGGTTCTATACTGTGATCTCATT
                  ************************************************************

HsCD00045169      TGCCTCGACTACGATGGAAACATTTTGGATGCCTGCACATTTGCTTTGCTAGCGGCTTTA
NM_181503.2       TGCCTCGACTACGATGGAAACATTTTGGATGCCTGCACATTTGCTTTGCTAGCGGCTTTA
                  ************************************************************

HsCD00045169      AAAAATGTACAGTTGCCTGAAGTTACTATAAATGAAGAAACTGCTTTAGCAGAAGTTAAT
NM_181503.2       AAAAATGTACAGTTGCCTGAAGTTACTATAAATGAAGAAACTGCTTTAGCAGAAGTTAAT
                  ************************************************************

HsCD00045169      TTAAAGAAGAAAAGTTATTTGAATATTAGAACTCATCCAGTTGCAACTTCCTTTGCTGTG
NM_181503.2       TTAAAGAAGAAAAGTTATTTGAATATTAGAACTCATCCAGTTGCAACTTCCTTTGCTGTG
                  ************************************************************

HsCD00045169      TTTGATGACACTTTGCTTATAGTTGACCCTACTGGAGAGGAGGAACATCTGGCAACAGGA
NM_181503.2       TTTGATGACACTTTGCTTATAGTTGACCCTACTGGAGAGGAGGAACATCTGGCAACAGGA
                  ************************************************************

HsCD00045169      ACCTTAACAATAGTAATGGATGAGGAAGGCAAACTCTGTTGTCTTCACAAACCAGGTGGA
NM_181503.2       ACCTTAACAATAGTAATGGATGAGGAAGGCAAACTCTGTTGTCTTCACAAACCAGGTGGA
                  ************************************************************

HsCD00045169      AGTGGGCTAACTGGAGCTAAACTTCAGGACTGTATGAGCCGAGCAGTTACAAGACACAAA
NM_181503.2       AGTGGGCTAACTGGAGCTAAACTTCAGGACTGTATGAGCCGAGCAGTTACAAGACACAAA
                  ************************************************************

HsCD00045169      GAAGTTAAAAAACTGATGGATGAAGTAATTAAGAGTATGAAACCCAAATAG
NM_181503.2       GAAGTTAAAAAACTGATGGATGAAGTAATTAAGAGTATGAAACCCAAATAA
                  **************************************************.

Pathways

Reactome : ATF4 activates genes
Reactome : Butyrate Response Factor 1 (BRF1) destabilizes mRNA
Reactome : Deadenylation-dependent mRNA decay
Reactome : Gene Expression
Reactome : KSRP destabilizes mRNA
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Metabolism of proteins
Reactome : PERK regulates gene expression
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Tristetraprolin (TTP) destabilizes mRNA
Reactome : Unfolded Protein Response (UPR)
Reactome : mRNA decay by 3' to 5' exoribonuclease

Protein

UniProt : Q96B26
HPRD : 09351

Clone

Cloning Information : 194106
HIP Master Clone ID : 168817
Original Clone ID : FLH194106.01X

Organism


TAX_ID : 9606
Species Specific ID: 11340

Genome

Chromosome : 13
Map Location : 13q13.1
Ensembl : ENSG00000120699

Reagents

Labome : EXOSC8-antibody