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RBMX (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RBMX
Gene Name:  RNA binding motif protein, X-linked
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH195282.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045239

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1176nts         Open reading frame : 1 to 1176
Reference Sequence Alignment

ATGGTTGAAGCAGATCGCCCAGGAAAGCTCTTCATTGGTGGGCTTAATACGGAAACAAAT
GAGAAAGCTCTTGAAGCAGTATTTGGCAAATATGGACGAATAGTGGAAGTACTCTTGATG
AAAGACCGTGAAACCAACAAATCAAGAGGATTTGCTTTTGTCACCTTTGAAAGCCCAGCA
GACGCTAAGGATGCAGCCAGAGACATGAATGGAAAGTCATTAGATGGAAAAGCCATCAAG
GTGGAACAAGCCACCAAACCATCATTTGAAAGTGGTAGACGTGGACCGCCTCCACCTCCA
AGAAGTAGAGGCCCTCCAAGAGGTCTTAGAGGTGGAAGAGGAGGAAGTGGAGGAACCAGG
GGACCTCCCTCACGGGGAGGACACATGGATGACGGTGGATATTCCATGAATTTTAACATG
AGTTCTTCCAGGGGACCACTCCCAGTAAAAAGAGGACCACCACCAAGAAGTGGGGGTCCT
CCTCCTAAGAGATCTGCACCTTCAGGACCAGTTCGCAGTAGCAGTGGAATGGGAGGAAGA
GCTCCTGTATCACGTGGAAGAGATAGTTATGGAGGTCCACCTCGAAGGGAACCGCTGCCC
TCTCGTAGAGATGTTTATTTGTCCCCAAGAGATGATGGGTATTCTACTAAAGACAGCTAT
TCAAGCAGAGATTACCCAAGTTCTCGTGATACTAGAGATTATGCACCACCACCACGAGAT
TATACTTACCGTGATTATGGTCATTCCAGTTCACGTGATGACTATCCATCAAGAGGATAT
AGCGATAGAGATGGATATGGTCGTGATCGTGACTATTCAGATCATCCAAGTGGAGGTTCC
TACAGAGATTCATATGAGAGTTATGGTAACTCACGTAGTGCTCCACCTACACGAGGGCCC
CCGCCATCTTATGGTGGAAGCAGTCGCTATGATGATTACAGCAGCTCACGTGACGGATAT
GGTGGAAGTCGAGACAGTTACTCAAGCAGCCGAAGTGATCTCTACTCAAGTGGTCGTGAT
CGGGTTGGCAGACAAGAAAGAGGGCTTCCCCCTTCTATGGAAAGGGGGTACCCTCCTCCA
CGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAGGTGGTGGCCGTGGAGGAAGC
CGATCTGATAGAGGGGGAGGCAGAAGCAGATACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1331
Start on reference sequence 156
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RBMX
Symbol Nomenclature : RBMX
SYNONYM : HNRNPG; HNRPG; RBMXP1; RBMXRT; RNMX; hnRNP-G
Designation : RNA binding motif protein, X chromosome|RNA-binding motif protein, X chromosome|glycoprotein p43|heterogeneous nuclear ribonucleoprotein G|hnRNP G
Full Nomenclature : RNA binding motif protein, X-linked
GENEID : 27316
Locus Tag : RP11-1114A5.1
HGNC : 9910
MIM : 300199
Vega : OTTHUMG00000022517
Target GenBank: BC007435

 

Reference Sequence Alignment

  

HsCD00045239      ATGGTTGAAGCAGATCGCCCAGGAAAGCTCTTCATTGGTGGGCTTAATACGGAAACAAAT
NM_002139.3       ATGGTTGAAGCAGATCGCCCAGGAAAGCTCTTCATTGGTGGGCTTAATACGGAAACAAAT
                  ************************************************************

HsCD00045239      GAGAAAGCTCTTGAAGCAGTATTTGGCAAATATGGACGAATAGTGGAAGTACTCTTGATG
NM_002139.3       GAGAAAGCTCTTGAAGCAGTATTTGGCAAATATGGACGAATAGTGGAAGTACTCTTGATG
                  ************************************************************

HsCD00045239      AAAGACCGTGAAACCAACAAATCAAGAGGATTTGCTTTTGTCACCTTTGAAAGCCCAGCA
NM_002139.3       AAAGACCGTGAAACCAACAAATCAAGAGGATTTGCTTTTGTCACCTTTGAAAGCCCAGCA
                  ************************************************************

HsCD00045239      GACGCTAAGGATGCAGCCAGAGACATGAATGGAAAGTCATTAGATGGAAAAGCCATCAAG
NM_002139.3       GACGCTAAGGATGCAGCCAGAGACATGAATGGAAAGTCATTAGATGGAAAAGCCATCAAG
                  ************************************************************

HsCD00045239      GTGGAACAAGCCACCAAACCATCATTTGAAAGTGGTAGACGTGGACCGCCTCCACCTCCA
NM_002139.3       GTGGAACAAGCCACCAAACCATCATTTGAAAGTGGTAGACGTGGACCGCCTCCACCTCCA
                  ************************************************************

HsCD00045239      AGAAGTAGAGGCCCTCCAAGAGGTCTTAGAGGTGGAAGAGGAGGAAGTGGAGGAACCAGG
NM_002139.3       AGAAGTAGAGGCCCTCCAAGAGGTCTTAGAGGTGGAAGAGGAGGAAGTGGAGGAACCAGG
                  ************************************************************

HsCD00045239      GGACCTCCCTCACGGGGAGGACACATGGATGACGGTGGATATTCCATGAATTTTAACATG
NM_002139.3       GGACCTCCCTCACGGGGAGGACACATGGATGACGGTGGATATTCCATGAATTTTAACATG
                  ************************************************************

HsCD00045239      AGTTCTTCCAGGGGACCACTCCCAGTAAAAAGAGGACCACCACCAAGAAGTGGGGGTCCT
NM_002139.3       AGTTCTTCCAGGGGACCACTCCCAGTAAAAAGAGGACCACCACCAAGAAGTGGGGGTCCT
                  ************************************************************

HsCD00045239      CCTCCTAAGAGATCTGCACCTTCAGGACCAGTTCGCAGTAGCAGTGGAATGGGAGGAAGA
NM_002139.3       CCTCCTAAGAGATCTGCACCTTCAGGACCAGTTCGCAGTAGCAGTGGAATGGGAGGAAGA
                  ************************************************************

HsCD00045239      GCTCCTGTATCACGTGGAAGAGATAGTTATGGAGGTCCACCTCGAAGGGAACCGCTGCCC
NM_002139.3       GCTCCTGTATCACGTGGAAGAGATAGTTATGGAGGTCCACCTCGAAGGGAACCGCTGCCC
                  ************************************************************

HsCD00045239      TCTCGTAGAGATGTTTATTTGTCCCCAAGAGATGATGGGTATTCTACTAAAGACAGCTAT
NM_002139.3       TCTCGTAGAGATGTTTATTTGTCCCCAAGAGATGATGGGTATTCTACTAAAGACAGCTAT
                  ************************************************************

HsCD00045239      TCAAGCAGAGATTACCCAAGTTCTCGTGATACTAGAGATTATGCACCACCACCACGAGAT
NM_002139.3       TCAAGCAGAGATTACCCAAGTTCTCGTGATACTAGAGATTATGCACCACCACCACGAGAT
                  ************************************************************

HsCD00045239      TATACTTACCGTGATTATGGTCATTCCAGTTCACGTGATGACTATCCATCAAGAGGATAT
NM_002139.3       TATACTTACCGTGATTATGGTCATTCCAGTTCACGTGATGACTATCCATCAAGAGGATAT
                  ************************************************************

HsCD00045239      AGCGATAGAGATGGATATGGTCGTGATCGTGACTATTCAGATCATCCAAGTGGAGGTTCC
NM_002139.3       AGCGATAGAGATGGATATGGTCGTGATCGTGACTATTCAGATCATCCAAGTGGAGGTTCC
                  ************************************************************

HsCD00045239      TACAGAGATTCATATGAGAGTTATGGTAACTCACGTAGTGCTCCACCTACACGAGGGCCC
NM_002139.3       TACAGAGATTCATATGAGAGTTATGGTAACTCACGTAGTGCTCCACCTACACGAGGGCCC
                  ************************************************************

HsCD00045239      CCGCCATCTTATGGTGGAAGCAGTCGCTATGATGATTACAGCAGCTCACGTGACGGATAT
NM_002139.3       CCGCCATCTTATGGTGGAAGCAGTCGCTATGATGATTACAGCAGCTCACGTGACGGATAT
                  ************************************************************

HsCD00045239      GGTGGAAGTCGAGACAGTTACTCAAGCAGCCGAAGTGATCTCTACTCAAGTGGTCGTGAT
NM_002139.3       GGTGGAAGTCGAGACAGTTACTCAAGCAGCCGAAGTGATCTCTACTCAAGTGGTCGTGAT
                  ************************************************************

HsCD00045239      CGGGTTGGCAGACAAGAAAGAGGGCTTCCCCCTTCTATGGAAAGGGGGTACCCTCCTCCA
NM_002139.3       CGGGTTGGCAGACAAGAAAGAGGGCTTCCCCCTTCTATGGAAAGGGGGTACCCTCCTCCA
                  ************************************************************

HsCD00045239      CGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAGGTGGTGGCCGTGGAGGAAGC
NM_002139.3       CGTGATTCCTACAGCAGTTCAAGCCGCGGAGCACCAAGAGGTGGTGGCCGTGGAGGAAGC
                  ************************************************************

HsCD00045239      CGATCTGATAGAGGGGGAGGCAGAAGCAGATACTAG
NM_002139.3       CGATCTGATAGAGGGGGAGGCAGAAGCAGATACTAG
                  ************************************

Pathways

Reactome : Gene Expression
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
SMART domain : RRM_1 : RNA recognition motif
UniProt : B4E3U4
UniProt : P38159
HPRD : 02186

Clone

Cloning Information : 195282
HIP Master Clone ID : 169993
Original Clone ID : FLH195282.01X

Organism


TAX_ID : 9606
Species Specific ID: 27316

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000147274

Reagents

Labome : RBMX-antibody