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all2095 (Nostoc sp. PCC 7120) in pMCSG7 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  all2095
Gene Name:  two-component sensor histidine kinase
Sequence             
Original Clone ID: APC67669.3_SG328_pMCSG7
Keyword: partial cds
Species: Nostoc sp. PCC 7120
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Midwest Center for Structural Genomics
Jessica Bearden
NsCD00452520

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 390nts         Open reading frame : 51 to 440

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTACGCAAAA
GTGAAGAACGATGGCAATTAGCGATCGTCGGCACTGATGAAGCCATTTGGGACTGGGATA
TACTGACTAATCATACCTATCGTTCAGACCGTTGGTTTGAAATGCTAGGCTATGAACGTC
ATGAAATGAGTACTTTTGATGATGAATGGAGTATCCGCATTCATCCTGATGATTATAACC
GAGTGATGGCTGCTCAAGCAGCTTATCTGCGTCGGGAAGTTCCAGTATATTACACTGAGT
ATCGTCTCCGATGCAAAGATGGTAACTACAAATGGTTTCGTTCACGGGCTAAAGGTGTTT
GGGATGAACAAGGCAACCCCATAAGATTGGTTGGTTCCTTGGGTGACATTACAGGACGTA
AAAGTATTGAATTAGACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCG
ACAAGCTTGCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GCC

3' Linker Sequence:
TAAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC67669.3
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : all2095
GENEID : 1105693
Locus Tag : all2095
GI : 17131186
Target GenBank: None

 

Clone

Cloning Information : 575981
HIP Master Clone ID : 481580
Original Clone ID : APC67669.3_SG328_pMCSG7

Experimental

TargetTrack - Experimental data : APC67669.3

Organism


TAX_ID : 103690
Species Specific ID: 1105693

Reagents

Labome : all2095-cDNA