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GPC2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GPC2
Gene Name:  glypican 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH196181.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00045342

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1740nts         Open reading frame : 1 to 1740
Reference Sequence Alignment

ATGTCCGCGCTGCGACCTCTCCTGCTTCTGCTGCTGCCTCTGTGTCCCGGTCCTGGTCCC
GGACCCGGGAGCGAGGCAAAGGTCACCCGGAGTTGTGCAGAGACCCGGCAGGTGCTGGGG
GCCCGGGGATATAGCTTAAACCTAATCCCTCCCGCCCTGATCTCAGGTGAGCACCTCCGG
GTCTGTCCCCAGGAGTACACCTGCTGTTCCAGTGAGACAGAGCAGAGGCTGATCAGGGAG
ACTGAGGCCACCTTCCGAGGCCTGGTGGAGGACAGCGGCTCCTTTCTGGTTCACACACTG
GCTGCCAGGCACAGAAAATTTGATGAGTTTTTTCTGGAGATGCTCTCAGTAGCCCAGCAC
TCTCTGACCCAGCTCTTCTCCCACTCCTACGGCCGCCTGTATGCCCAGCACGCCCTCATA
TTCAATGGCCTGTTCTCTCGGCTGCGAGACTTCTATGGGGAATCTGGTGAGGGGTTGGAT
GACACCCTGGCGGATTTCTGGGCACAGCTCCTGGAGAGAGTGTTCCCGCTGCTGCACCCA
CAGTACAGCTTCCCCCCTGACTACCTGCTCTGCCTCTCACGCTTGGCCTCATCTACCGAT
GGCTCTCTGCAGCCCTTTGGGGACTCACCCCGCCGCCTCCGCCTGCAGATAACCCGGACC
CTGGTGGCTGCCCGAGCCTTTGTGCAGGGCCTGGAGACTGGAAGAAATGTGGTCAGCGAA
GCGCTTAAGGTGCCGGTGTCTGAAGGCTGCAGCCAGGCTCTGATGCGTCTCATCGGCTGT
CCCCTGTGCCGGGGGGTCCCCTCACTTATGCCCTGCCAGGGCTTCTGCCTCAACGTGGTT
CGTGGCTGTCTCAGCAGCAGGGGACTGGAGCCTGACTGGGGCAACTATCTGGATGGTCTC
CTGATCCTGGCTGATAAGCTCCAGGGCCCCTTTTCCTTTGAGCTGACGGCCGAGTCCATT
GGGGTGAAGATCTCGGAGGGTTTGATGTACCTGCAGGAAAACAGTGCGAAGGTGTCCGCC
CAGGTGTTTCAGGAGTGCGGCCCCCCCGACCCGGTGCCTGCCCGCAACCGTCGAGCCCCG
CCGCCCCGGGAAGAGGCGGGCCGGCTGTGGTCGATGGTGACCGAGGAGGAGCGGCCCACG
ACGGCCGCAGGCACCAACCTGCACCGGCTGGTGTGGGAGCTCCGCGAGCGTCTGGCCCGG
ATGCGGGGCTTCTGGGCCCGGCTGTCCCTGACGGTGTGCGGAGACTCTCGCATGGCAGCG
GACGCCTCGCTGGAGGCGGCGCCCTGCTGGACCGGAGCCGGGCGGGGCCGGTACTTGCCG
CCAGTGGTCGGGGGCTCCCCGGCCGAGCAGGTCAACAACCCCGAGCTCAAGGTGGACGCC
TCGGGCCCCGATGTCCCGACACGGCGGCGTCGGCTACAGCTCCGGGCGGCCACGGCCAGA
ATGAAAACGGCCGCACTGGGACACGACCTGGACGGGCAGGACGCGGATGAGGATGCCAGC
GGCTCTGGAGGGGGACAGCAGTATGCAGATGACTGGATGGCTGGGGCTGTGGCTCCCCCA
GCCCGGCCTCCTCGGCCTCCATACCCTCCTAGAAGGGATGGTTCTGGGGGCAAAGGAGGA
GGTGGCAGTGCCCGCTACAACCAGGGCCGGAGCAGGAGTGGGGGGGCATCTATTGGTTTT
CACACCCAAACCATCCTCATTCTCTCCCTCTCAGCCCTGGCCCTGCTTGGACCTCGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1908
Start on reference sequence 169
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPC2
Symbol Nomenclature : GPC2
Designation : cerebroglycan|glypican proteoglycan 2, cerebroglycan proteoglycan|glypican-2
Full Nomenclature : glypican 2
GENEID : 221914
HGNC : 4450
Vega : OTTHUMG00000154894
Target GenBank: BC027972

 

Reference Sequence Alignment

  

HsCD00045342      ATGTCCGCGCTGCGACCTCTCCTGCTTCTGCTGCTGCCTCTGTGTCCCGGTCCTGGTCCC
NM_152742.1       ATGTCCGCGCTGCGACCTCTCCTGCTTCTGCTGCTGCCTCTGTGTCCCGGTCCTGGTCCC
                  ************************************************************

HsCD00045342      GGACCCGGGAGCGAGGCAAAGGTCACCCGGAGTTGTGCAGAGACCCGGCAGGTGCTGGGG
NM_152742.1       GGACCCGGGAGCGAGGCAAAGGTCACCCGGAGTTGTGCAGAGACCCGGCAGGTGCTGGGG
                  ************************************************************

HsCD00045342      GCCCGGGGATATAGCTTAAACCTAATCCCTCCCGCCCTGATCTCAGGTGAGCACCTCCGG
NM_152742.1       GCCCGGGGATATAGCTTAAACCTAATCCCTCCCGCCCTGATCTCAGGTGAGCACCTCCGG
                  ************************************************************

HsCD00045342      GTCTGTCCCCAGGAGTACACCTGCTGTTCCAGTGAGACAGAGCAGAGGCTGATCAGGGAG
NM_152742.1       GTCTGTCCCCAGGAGTACACCTGCTGTTCCAGTGAGACAGAGCAGAGGCTGATCAGGGAG
                  ************************************************************

HsCD00045342      ACTGAGGCCACCTTCCGAGGCCTGGTGGAGGACAGCGGCTCCTTTCTGGTTCACACACTG
NM_152742.1       ACTGAGGCCACCTTCCGAGGCCTGGTGGAGGACAGCGGCTCCTTTCTGGTTCACACACTG
                  ************************************************************

HsCD00045342      GCTGCCAGGCACAGAAAATTTGATGAGTTTTTTCTGGAGATGCTCTCAGTAGCCCAGCAC
NM_152742.1       GCTGCCAGGCACAGAAAATTTGATGAGTTTTTTCTGGAGATGCTCTCAGTAGCCCAGCAC
                  ************************************************************

HsCD00045342      TCTCTGACCCAGCTCTTCTCCCACTCCTACGGCCGCCTGTATGCCCAGCACGCCCTCATA
NM_152742.1       TCTCTGACCCAGCTCTTCTCCCACTCCTACGGCCGCCTGTATGCCCAGCACGCCCTCATA
                  ************************************************************

HsCD00045342      TTCAATGGCCTGTTCTCTCGGCTGCGAGACTTCTATGGGGAATCTGGTGAGGGGTTGGAT
NM_152742.1       TTCAATGGCCTGTTCTCTCGGCTGCGAGACTTCTATGGGGAATCTGGTGAGGGGTTGGAT
                  ************************************************************

HsCD00045342      GACACCCTGGCGGATTTCTGGGCACAGCTCCTGGAGAGAGTGTTCCCGCTGCTGCACCCA
NM_152742.1       GACACCCTGGCGGATTTCTGGGCACAGCTCCTGGAGAGAGTGTTCCCGCTGCTGCACCCA
                  ************************************************************

HsCD00045342      CAGTACAGCTTCCCCCCTGACTACCTGCTCTGCCTCTCACGCTTGGCCTCATCTACCGAT
NM_152742.1       CAGTACAGCTTCCCCCCTGACTACCTGCTCTGCCTCTCACGCTTGGCCTCATCTACCGAT
                  ************************************************************

HsCD00045342      GGCTCTCTGCAGCCCTTTGGGGACTCACCCCGCCGCCTCCGCCTGCAGATAACCCGGACC
NM_152742.1       GGCTCTCTGCAGCCCTTTGGGGACTCACCCCGCCGCCTCCGCCTGCAGATAACCCGGACC
                  ************************************************************

HsCD00045342      CTGGTGGCTGCCCGAGCCTTTGTGCAGGGCCTGGAGACTGGAAGAAATGTGGTCAGCGAA
NM_152742.1       CTGGTGGCTGCCCGAGCCTTTGTGCAGGGCCTGGAGACTGGAAGAAATGTGGTCAGCGAA
                  ************************************************************

HsCD00045342      GCGCTTAAGGTGCCGGTGTCTGAAGGCTGCAGCCAGGCTCTGATGCGTCTCATCGGCTGT
NM_152742.1       GCGCTTAAGGTGCCGGTGTCTGAAGGCTGCAGCCAGGCTCTGATGCGTCTCATCGGCTGT
                  ************************************************************

HsCD00045342      CCCCTGTGCCGGGGGGTCCCCTCACTTATGCCCTGCCAGGGCTTCTGCCTCAACGTGGTT
NM_152742.1       CCCCTGTGCCGGGGGGTCCCCTCACTTATGCCCTGCCAGGGCTTCTGCCTCAACGTGGTT
                  ************************************************************

HsCD00045342      CGTGGCTGTCTCAGCAGCAGGGGACTGGAGCCTGACTGGGGCAACTATCTGGATGGTCTC
NM_152742.1       CGTGGCTGTCTCAGCAGCAGGGGACTGGAGCCTGACTGGGGCAACTATCTGGATGGTCTC
                  ************************************************************

HsCD00045342      CTGATCCTGGCTGATAAGCTCCAGGGCCCCTTTTCCTTTGAGCTGACGGCCGAGTCCATT
NM_152742.1       CTGATCCTGGCTGATAAGCTCCAGGGCCCCTTTTCCTTTGAGCTGACGGCCGAGTCCATT
                  ************************************************************

HsCD00045342      GGGGTGAAGATCTCGGAGGGTTTGATGTACCTGCAGGAAAACAGTGCGAAGGTGTCCGCC
NM_152742.1       GGGGTGAAGATCTCGGAGGGTTTGATGTACCTGCAGGAAAACAGTGCGAAGGTGTCCGCC
                  ************************************************************

HsCD00045342      CAGGTGTTTCAGGAGTGCGGCCCCCCCGACCCGGTGCCTGCCCGCAACCGTCGAGCCCCG
NM_152742.1       CAGGTGTTTCAGGAGTGCGGCCCCCCCGACCCGGTGCCTGCCCGCAACCGTCGAGCCCCG
                  ************************************************************

HsCD00045342      CCGCCCCGGGAAGAGGCGGGCCGGCTGTGGTCGATGGTGACCGAGGAGGAGCGGCCCACG
NM_152742.1       CCGCCCCGGGAAGAGGCGGGCCGGCTGTGGTCGATGGTGACCGAGGAGGAGCGGCCCACG
                  ************************************************************

HsCD00045342      ACGGCCGCAGGCACCAACCTGCACCGGCTGGTGTGGGAGCTCCGCGAGCGTCTGGCCCGG
NM_152742.1       ACGGCCGCAGGCACCAACCTGCACCGGCTGGTGTGGGAGCTCCGCGAGCGTCTGGCCCGG
                  ************************************************************

HsCD00045342      ATGCGGGGCTTCTGGGCCCGGCTGTCCCTGACGGTGTGCGGAGACTCTCGCATGGCAGCG
NM_152742.1       ATGCGGGGCTTCTGGGCCCGGCTGTCCCTGACGGTGTGCGGAGACTCTCGCATGGCAGCG
                  ************************************************************

HsCD00045342      GACGCCTCGCTGGAGGCGGCGCCCTGCTGGACCGGAGCCGGGCGGGGCCGGTACTTGCCG
NM_152742.1       GACGCCTCGCTGGAGGCGGCGCCCTGCTGGACCGGAGCCGGGCGGGGCCGGTACTTGCCG
                  ************************************************************

HsCD00045342      CCAGTGGTCGGGGGCTCCCCGGCCGAGCAGGTCAACAACCCCGAGCTCAAGGTGGACGCC
NM_152742.1       CCAGTGGTCGGGGGCTCCCCGGCCGAGCAGGTCAACAACCCCGAGCTCAAGGTGGACGCC
                  ************************************************************

HsCD00045342      TCGGGCCCCGATGTCCCGACACGGCGGCGTCGGCTACAGCTCCGGGCGGCCACGGCCAGA
NM_152742.1       TCGGGCCCCGATGTCCCGACACGGCGGCGTCGGCTACAGCTCCGGGCGGCCACGGCCAGA
                  ************************************************************

HsCD00045342      ATGAAAACGGCCGCACTGGGACACGACCTGGACGGGCAGGACGCGGATGAGGATGCCAGC
NM_152742.1       ATGAAAACGGCCGCACTGGGACACGACCTGGACGGGCAGGACGCGGATGAGGATGCCAGC
                  ************************************************************

HsCD00045342      GGCTCTGGAGGGGGACAGCAGTATGCAGATGACTGGATGGCTGGGGCTGTGGCTCCCCCA
NM_152742.1       GGCTCTGGAGGGGGACAGCAGTATGCAGATGACTGGATGGCTGGGGCTGTGGCTCCCCCA
                  ************************************************************

HsCD00045342      GCCCGGCCTCCTCGGCCTCCATACCCTCCTAGAAGGGATGGTTCTGGGGGCAAAGGAGGA
NM_152742.1       GCCCGGCCTCCTCGGCCTCCATACCCTCCTAGAAGGGATGGTTCTGGGGGCAAAGGAGGA
                  ************************************************************

HsCD00045342      GGTGGCAGTGCCCGCTACAACCAGGGCCGGAGCAGGAGTGGGGGGGCATCTATTGGTTTT
NM_152742.1       GGTGGCAGTGCCCGCTACAACCAGGGCCGGAGCAGGAGTGGGGGGGCATCTATTGGTTTT
                  ************************************************************

HsCD00045342      CACACCCAAACCATCCTCATTCTCTCCCTCTCAGCCCTGGCCCTGCTTGGACCTCGATAG
NM_152742.1       CACACCCAAACCATCCTCATTCTCTCCCTCTCAGCCCTGGCCCTGCTTGGACCTCGATAA
                  ***********************************************************.

Pathways

NCI : Glypican 2 network
NCI : Glypican pathway
Reactome : A tetrasaccharide linker sequence is required for GAG synthesis
Reactome : Chondroitin sulfate/dermatan sulfate metabolism
Reactome : Disease
Reactome : Diseases associated with visual transduction
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : HS-GAG biosynthesis
Reactome : HS-GAG degradation
Reactome : Heparan sulfate/heparin (HS-GAG) metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora
Reactome : Retinoid metabolism and transport
Reactome : Signal Transduction
Reactome : Visual phototransduction

Protein

UniProt : Q8N158
HPRD : 17051

Clone

Cloning Information : 196181
HIP Master Clone ID : 170892
Original Clone ID : FLH196181.01X

Organism


TAX_ID : 9606
Species Specific ID: 221914

Genome

Chromosome : 7
Map Location : 7q22.1
Ensembl : ENSG00000213420

Reagents

Labome : GPC2-antibody