| Gene: |
Gene Symbol:
CDK1 Gene Name: cyclin-dependent kinase 1 Sequence Map: pDNR-Dual_with_human_insert.pdf |
|---|---|
| Original Clone ID: | FLH026553.01X |
| Keyword: | None |
| Species: | Homo sapiens |
| Type: | cDNA |
| Vector Name: | pDNR-Dual Format: CLOSED |
| Source: | HIP |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / No |
| Publications: |
PMID: 15928075 Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation. |
| Authors: |
HIP |
|
HsCD00004538 Price: Login for Pricing AVAILABLE No restriction Special MTA: None |
|
Insert sequence: 894nts
Open reading frame : 1 to 894 Reference Sequence Alignment ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG
GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA
GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT
CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT
GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG
GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC
TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA
ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT
ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT
TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG
AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG
GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA
TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC
TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG
GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Reference Sequence Annotations: Start on reference sequence 14 End on reference sequence 907 |
|
|
Mutations: None |
Discrepancy: None |
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC GACACC(ATG-GOI) |
|
3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC |
|
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
| Protein Expression Results: | None |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pDNR-Dual |
pDNR-Dual in advanced viewed |
| Synonyms: | '' | |
| Sequencing Primer: | Forward:
M13F
Reverse: Unknown |
|
| Description: | Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from). | |
| Comments: | The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added. | |
| Size (bp): | 4938 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker | |
| Author Name: |
BD Biosciences Clontech |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| MCS | MCS | restriction enzyme-type multiple cloning site | 45 | 129 |
| bacterial origin | ColE1 | ColE1 (pUC-type) origin of replication | 4111 | 4574 |
| negative selection marker | SacB | SacB negative selection marker gene (sucrose sensitivity) | 1057 | 2972 |
| primer site | M13 primer | M13 primer sequencing site | 4839 | 4855 |
| primer site | T7 primer | T7 RNA Pol primer sequencing site | 4865 | 4883 |
| recombination site | loxP site | loxP recombination site | 9 | 42 |
| recombination site | loxP site | loxP recombination site | 1015 | 1048 |
| selectable marker | CmR | chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) | 986 | 327 |
| selectable marker | AmpR | ampicillin resistance gene | 3103 | 3963 |
| splice donor site | SD site | splice donor site | 132 | 136 |
| tag | HN | 6xHN epitope tag | 141 | 176 |
- Gene
- Pathways
- Protein
- Clone
- Organism
- Genome
- Reagents
Gene
| Gene Symbol : | CDK1 |
|---|---|
| Symbol Nomenclature : | CDK1 |
| SYNONYM : | CDC2; CDC28A; P34CDC2 |
| Designation : | cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase |
| Full Nomenclature : | cyclin-dependent kinase 1 |
| GENEID : | 983 |
| GI : | 61362000 |
| GenBank Accession : | AY890196 |
| HGNC : | 1722 |
| MIM : | 116940 |
| Vega : | OTTHUMG00000018290 |
| Target GenBank: | BC014563 |
Reference Sequence Alignment
HsCD00004538 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG
XM_005270303.1 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG
NM_001786.4 ATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGGAGTTGTGTATAAG
************************************************************
HsCD00004538 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA
XM_005270303.1 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA
NM_001786.4 GGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAGACTAGAAAGTGAA
************************************************************
HsCD00004538 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT
XM_005270303.1 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT
NM_001786.4 GAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAAGGAACTTCGTCAT
************************************************************
HsCD00004538 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT
XM_005270303.1 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT
NM_001786.4 CCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTTATATCTCATCTTT
************************************************************
HsCD00004538 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG
XM_005270303.1 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG
NM_001786.4 GAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCCTGGTCAGTACATG
************************************************************
HsCD00004538 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC
XM_005270303.1 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC
NM_001786.4 GATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGATTGTGTTTTGTCAC
************************************************************
HsCD00004538 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA
XM_005270303.1 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA
NM_001786.4 TCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGATTGATGACAAAGGA
************************************************************
HsCD00004538 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT
XM_005270303.1 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT
NM_001786.4 ACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACCTATCAGAGTATAT
************************************************************
HsCD00004538 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT
XM_005270303.1 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT
NM_001786.4 ACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCTGGGGTCAGCTCGT
************************************************************
HsCD00004538 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG
XM_005270303.1 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG
NM_001786.4 TACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGAACTAGCAACTAAG
************************************************************
HsCD00004538 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG
XM_005270303.1 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG
NM_001786.4 AAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGATTTTCAGAGCTTTG
************************************************************
HsCD00004538 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA
XM_005270303.1 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA
NM_001786.4 GGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGACTATAAGAATACA
************************************************************
HsCD00004538 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC
XM_005270303.1 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC
NM_001786.4 TTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTTGGATGAAAATGGC
************************************************************
HsCD00004538 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG
XM_005270303.1 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG
NM_001786.4 TTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAATTTCTGGCAAAATG
************************************************************
HsCD00004538 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG
XM_005270303.1 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG
NM_001786.4 GCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAAGATGTAG
******************************************************
Pathways
| NCI : | AP-1 transcription factor network |
|---|---|
| NCI : | E2F transcription factor network |
| NCI : | FOXM1 transcription factor network |
| NCI : | p73 transcription factor network |
| NCI : | PLK1 signaling events |
| NCI : | Retinoic acid receptors-mediated signaling |
| Reactome : | Activated TLR4 signalling |
| Reactome : | Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins |
| Reactome : | Activation of NIMA Kinases NEK9, NEK6, NEK7 |
| Reactome : | APC/C-mediated degradation of cell cycle proteins |
| Reactome : | APC/C:Cdc20 mediated degradation of Cyclin B |
| Reactome : | APC/C:Cdc20 mediated degradation of mitotic proteins |
| Reactome : | ARMS-mediated activation |
| Reactome : | Axon guidance |
| Reactome : | Cdc20:Phospho-APC/C mediated degradation of Cyclin A |
| Reactome : | Cell Cycle |
| Reactome : | Cell Cycle Checkpoints |
| Reactome : | Cell Cycle, Mitotic |
| Reactome : | Centrosome maturation |
| Reactome : | Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex |
| Reactome : | Condensation of Prometaphase Chromosomes |
| Reactome : | Condensation of Prophase Chromosomes |
| Reactome : | Cyclin A/B1 associated events during G2/M transition |
| Reactome : | Cyclin B2 mediated events |
| Reactome : | Cytokine Signaling in Immune system |
| Reactome : | DAP12 interactions |
| Reactome : | DAP12 signaling |
| Reactome : | Depolymerisation of the Nuclear Lamina |
| Reactome : | Developmental Biology |
| Reactome : | Disease |
| Reactome : | Downstream signal transduction |
| Reactome : | Downstream signaling of activated FGFR |
| Reactome : | E2F mediated regulation of DNA replication |
| Reactome : | E2F-enabled inhibition of pre-replication complex formation |
| Reactome : | ERK activation |
| Reactome : | ERK1 activation |
| Reactome : | Fc epsilon receptor (FCERI) signaling |
| Reactome : | FCERI mediated MAPK activation |
| Reactome : | Frs2-mediated activation |
| Reactome : | FRS2-mediated cascade |
| Reactome : | G0 and Early G1 |
| Reactome : | G1/S Transition |
| Reactome : | G1/S-Specific Transcription |
| Reactome : | G2/M Checkpoints |
| Reactome : | G2/M DNA damage checkpoint |
| Reactome : | G2/M DNA replication checkpoint |
| Reactome : | G2/M Transition |
| Reactome : | Gastrin-CREB signalling pathway via PKC and MAPK |
| Reactome : | Golgi Cisternae Pericentriolar Stack Reorganization |
| Reactome : | GRB2 events in EGFR signaling |
| Reactome : | GRB2 events in ERBB2 signaling |
| Reactome : | IGF1R signaling cascade |
| Reactome : | Immune System |
| Reactome : | Innate Immune System |
| Reactome : | Insulin receptor signalling cascade |
| Reactome : | Interleukin-2 signaling |
| Reactome : | IRS-mediated signalling |
| Reactome : | IRS-related events |
| Reactome : | IRS-related events triggered by IGF1R |
| Reactome : | Loss of Nlp from mitotic centrosomes |
| Reactome : | Loss of proteins required for interphase microtubule organizationÿ from the centrosome |
| Reactome : | M Phase |
| Reactome : | MAP kinase activation in TLR cascade |
| Reactome : | MASTL Facilitates Mitotic Progression |
| Reactome : | Mitotic G1-G1/S phases |
| Reactome : | Mitotic G2-G2/M phases |
| Reactome : | Mitotic M-M/G1 phases |
| Reactome : | Mitotic Prometaphase |
| Reactome : | Mitotic Prophase |
| Reactome : | MyD88 cascade initiated on plasma membrane |
| Reactome : | MyD88 dependent cascade initiated on endosome |
| Reactome : | MyD88-independent cascade |
| Reactome : | MyD88:Mal cascade initiated on plasma membrane |
| Reactome : | NCAM signaling for neurite out-growth |
| Reactome : | NGF signalling via TRKA from the plasma membrane |
| Reactome : | Nuclear Envelope Breakdown |
| Reactome : | Nuclear Pore Complex (NPC) Disassembly |
| Reactome : | Phosphorylation of Emi1 |
| Reactome : | Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes |
| Reactome : | Phosphorylation of the APC/C |
| Reactome : | Prolonged ERK activation events |
| Reactome : | RAF/MAP kinase cascade |
| Reactome : | Recruitment of mitotic centrosome proteins and complexes |
| Reactome : | Recruitment of NuMA to mitotic centrosomes |
| Reactome : | Regulation of APC/C activators between G1/S and early anaphase |
| Reactome : | Regulation of mitotic cell cycle |
| Reactome : | Regulation of PLK1 Activity at G2/M Transition |
| Reactome : | Resolution of Sister Chromatid Cohesion |
| Reactome : | SHC-mediated signalling |
| Reactome : | SHC-related events |
| Reactome : | SHC-related events triggered by IGF1R |
| Reactome : | SHC1 events in EGFR signaling |
| Reactome : | SHC1 events in ERBB2 signaling |
| Reactome : | SHC1 events in ERBB4 signaling |
| Reactome : | Signal Transduction |
| Reactome : | Signaling by EGFR |
| Reactome : | Signaling by EGFR in Cancer |
| Reactome : | Signaling by ERBB2 |
| Reactome : | Signaling by ERBB4 |
| Reactome : | Signaling by FGFR |
| Reactome : | Signaling by FGFR in disease |
| Reactome : | Signaling by GPCR |
| Reactome : | Signaling by Insulin receptor |
| Reactome : | Signaling by Interleukins |
| Reactome : | Signaling by Leptin |
| Reactome : | Signaling by PDGF |
| Reactome : | Signaling by SCF-KIT |
| Reactome : | Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) |
| Reactome : | Signalling by NGF |
| Reactome : | Signalling to ERKs |
| Reactome : | Signalling to p38 via RIT and RIN |
| Reactome : | Signalling to RAS |
| Reactome : | SOS-mediated signalling |
| Reactome : | Toll Like Receptor 10 (TLR10) Cascade |
| Reactome : | Toll Like Receptor 2 (TLR2) Cascade |
| Reactome : | Toll Like Receptor 3 (TLR3) Cascade |
| Reactome : | Toll Like Receptor 4 (TLR4) Cascade |
| Reactome : | Toll Like Receptor 5 (TLR5) Cascade |
| Reactome : | Toll Like Receptor 7/8 (TLR7/8) Cascade |
| Reactome : | Toll Like Receptor 9 (TLR9) Cascade |
| Reactome : | Toll Like Receptor TLR1:TLR2 Cascade |
| Reactome : | Toll Like Receptor TLR6:TLR2 Cascade |
| Reactome : | Toll-Like Receptors Cascades |
| Reactome : | TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation |
| Reactome : | TRAF6 Mediated Induction of proinflammatory cytokines |
| Reactome : | TRIF-mediated TLR3/TLR4 signaling |
| WikiPathway : | Cell cycle |
| WikiPathway : | DNA damage response |
| WikiPathway : | G1 to S cell cycle control |
| WikiPathway : | Integrated Cancer pathway |
| WikiPathway : | miRNA regulation of DNA Damage Response |
| WikiPathway : | TGF beta Signaling Pathway |
Protein
| SMART domain : | TyrKc : Tyrosine kinase, catalytic domain |
|---|---|
| SMART domain : | S_TKc : Serine/Threonine protein kinases, catalytic domain |
| UniProt : | P06493 |
| UniProt : | B7Z3D6 |
| HPRD : | 00302 |
Clone
| Cloning Information : | 26553 |
|---|---|
| HIP Master Clone ID : | 56929 |
| Original Clone ID : | FLH026553.01X |
Organism
| TAX_ID : | 9606 |
|---|---|
| Species Specific ID: | 983 |
Genome
| Chromosome : | 10 |
|---|---|
| Map Location : | 10q21.1 |
| Ensembl : | ENSG00000170312 |
Reagents
| Labome : | Cdc2-antibody |
|---|
