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Plim_1758 (Planctomyces limnophilus) in pMCSG7 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Plim_1758
Gene Name:  regulatory protein DeoR
Sequence             
Original Clone ID: APC100546.102
Keyword: None
Species: Planctomyces limnophilus
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Midwest Center for Structural Genomics
Jessica Bearden
PSI:Biology
PlCD00454527

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 750nts         Open reading frame : 40 to 789

GATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCTTTTGGATCAACGCCGT
CGACATGTGCTCGAACTCATTGAAGAAAAAGGCTTTATATCGCTTCATGAGCTTGCGACC
AAAACCGGGGTCAGCGAGTCAACACTGAGGCGGGATTTGGAATATCTTGACGGAATTCGG
CAGGTTCGCCGCACACGAGGCGGGGCTGCCTATGTCGGTGAATCGGTTGTTTCTCTGGAA
GAGCGAAGTGTGACCTCTCTGGTCGAGAAGCAGAGAATTGCTCGTTCGATCTCGGAAACG
ATAGGAAGTGGTGAAACAGTCCTGCTCGATGGGGGAACGACAACGCTTGAGGTGGCTCGT
GCCTTGATTGGCAAAGAACTGCAAGTTGTGACCAATTCCCTGGCGATTGCCAATTTGCTG
GTGAACAGCCCGGGTGTGGAATTGATTTTTTTAGGAGGGTATCTGCATCCCAAAACAGGT
GTGACTTTAGGCCCGCTCCTGAATTTGGCGTTATCGCAATTGCAGGTCCCTCGCATGGTT
TTCAGTGTCGGTGGCGTGACGGAGAAAGGCTTGTTCAACAGCAACACCCTCCTCGTTGAG
GCCGAGCGGCGGATGATTGATGCCGCTGAACGAGTGGTGCTGGCGGTTGATAGTCGGAAG
TTTGGAAAAGCAGCCCTCTCCCCACTTTGTCCACTCGACCGTGTTCATGAAATTGTGACA
GACGAAGGGATTCCCGAAGATTGGCGAAAGCGGATTGAAGACTTGGGAATTGAACTGCGA
ATTGCGTAACATTGGAAGTGGATAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100546
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : Plim_1758
GENEID : 9138459
Locus Tag : Plim_1758
GI : 296014349
Target GenBank: None

 

Protein

SMART domain : HTH_DEOR : helix_turn_helix, Deoxyribose operon repressor
SMART domain : DeoRC : DeoR C terminal sensor domain
Structural Annotation Wiki : TOPSAN
Structural Annotation Wiki : MCSG-APC100546

Clone

Cloning Information : 583972
HIP Master Clone ID : 486901
Original Clone ID : APC100546.102

Experimental

TargetTrack - Experimental data : MCSG-APC100546

Organism


TAX_ID : 521674
Species Specific ID: 9138459

Reagents

Labome : Plim_1758-cDNA