DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pMCSG54

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG54              
Source: Midwest Center for Structural Genomics
Description: Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning.
Comments: None
Publications: None
Authors: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00454845

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pMCSG54

pMCSG54 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and N-terminal Flag and 6xHiis tag followed by TEV protease cleavage site ; ampicillin resistance in bacteria; ligation independent cloning.
Comments: origin of replication similar to pUC vectors
Size (bp): 4302
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2295 2883
ligation independent cloning LIC ligation independent cloning site 204 235
protease cleavage site TEV TEV protease cleavage site 223 243
repressor binding site lac operator LacO fragment 359 381
repressor protein gene LacI Lac I 914 1873
selectable marker AmpR Ampicillin resistance 3054 3914
tag Flag N-terminal Flag tag 250 273
tag His N-terminal 6xHis tag 292 309
trxn termination sequence T7 term T7 terminator 26 72

 

Species Specific ID: None
Target GenBank: None