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FGD2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGD2
Gene Name:  FYVE, RhoGEF and PH domain containing 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH182471.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00045629

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1968nts         Open reading frame : 1 to 1968
Reference Sequence Alignment

ATGAAGGGGGCAAGTGAGGAGAAGCTGGCATCTGTGTCCAACCTGGTCACTGTGTTTGAG
AATAGCAGGACCCCAGAAGCAGCACCCAGAGGCCACAGGCTAGAGGACGTGCATCACCGC
CCTGAGTGCAGGCCTCCCGAGTCCCCAGGACCACGGGAGAAGACGAATGTCGGGGAGGCC
GTGGGGTCTGAGCCCAGGACAGTCAGCAGGAGGTACCTGAACTCCCTGAAGAACAAGCTG
TCCAGCGAAGCCTGGAGGAAATCTTGCCAGCCTGTGACCCTCTCAGGATCGGGGACGCAG
GAGCCAGAGAAGAAGATCGTCCAGGAGCTGCTGGAGACAGAGCAGGCCTATGTGGCGCGC
CTCCACCTGCTAGACCAGGTGTTTTTCCAGGAGCTGCTGAAGACAGCCCGCAGCAGCAAG
GCCTTCCCAGAGGATGTGGTCAGGGTCATCTTCTCCAACATCTCCTCCATCTATCAGTTC
CATTCTCAGTTCTTCCTCCCAGAGCTGCAGCGGCGCCTGGACGACTGGACAGCTAACCCC
CGCATCGGTGACGTGATCCAGAAGCTGGCCCCCTTCCTGAAGATGTACAGTGAGTATGTC
AAGAACTTTGAGCGAGCGGCTGAGCTGCTGGCCACCTGGACCGACAAGTCTCCACTCTTC
CAGGAGGTTCTCACTCGCATCCAGAGCAGCGAGGCTTCGGGCAGCCTGACCCTGCAGCAC
CACATGCTGGAACCAGTGCAGAGAATTCCACGTTACGAGCTGCTGCTCAAGGAGTACATC
CAGAAGCTGCCAGCCCAGGCCCCAGACCAGGCCGATGCCCAGAAAGCCCTGGACATGATC
TTCTCAGCTGCCCAGCACTCCAATGCAGCCATCACTGAGAGGGAGCGGCTGCAGGACCTG
TGGGAGGTGTACCAGCGCCTGGGCCTCGAGGACGACATAGTAGACCCCTCTAACACCCTG
CTCCGTGAGGGCCCGGTCCTCAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTAC
CTTTTCTTGTTCAACAACATGCTGCTCTACTGTGTGCCCAGGGTGATCCAGGTGGGCGCC
CAGTTCCAGGTGAGGACCCGCATCGATGTGGCCGGGATGAAGGTGCGGGAGCTGATGGAT
GCTGAGTTTCCCCACTCCTTCCTGGTGTCCGGGAAGCAGCGCACCCTGGAGCTGCAAGCC
CGGTCCCAGGAGGAAATGATTTCCTGGATGCAGGCCTTCCAAGCAGCCATTGACCAAATC
GAGAAGCGGAATGAAACCTTCAAGGCTGCGGCCCAGGGGCCTGAGGGAGACATCCAGGAG
CAGGAGCTGCAGTCTGAGGAGCTGGGCCTCCGGGCACCGCAGTGGGTCCGGGACAAGATG
GTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAACGCTCTGACGCGCCGTCGCCACCAC
TGCCGGGCCTGCGGCTATGTGGTGTGTGCCAGGTGCTCCGACTACCGGGCCGAACTGAAA
TACGACGACAACAGGCCCAACCGAGTCTGCCTCCACTGCTACGCATTCCTCACTGGAAAT
GTGCTGCCTGAGGCCAAGGAGGACAAGAGGCGGGGCATCCTGGAGAAAGGGTCCTCAGCC
ACGCCTGACCAGAGCCTGATGTGCAGCTTCCTGCAGCTCATCGGGGACAAGTGGGGCAAG
AGCGGCCCCCGGGGCTGGTGTGTGATCCCTCGGGATGACCCCCTCGTGCTCTATGTCTAT
GCTGCCCCTCAGGACATGAGGGCTCACACCTCCATCCCCCTGCTGGGCTACCAGGTGACT
GTTGGGCCCCAGGGGGACCCTCGGGTCTTCCAGCTACAGCAGTCAGGCCAGCTCTACACC
TTCAAGGCCGAGACGGAGGAGCTGAAGGGCCGCTGGGTGAAGGCCATGGAGCGGGCGGCC
AGTGGCTGGAGCCCCAGCTGGCCCAACGATGGGGACCTGTCCGACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2139
Start on reference sequence 172
Mutations:
None
Discrepancy:
t881g,M294R
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGD2
Symbol Nomenclature : FGD2
SYNONYM : ZFYVE4
Designation : FGD1 family, member 2|FLJ00276 protein|FYVE, RhoGEF and PH domain-containing protein 2|zinc finger FYVE domain-containing protein 4
Full Nomenclature : FYVE, RhoGEF and PH domain containing 2
GENEID : 221472
Locus Tag : RP3-405J24.2
HGNC : 3664
MIM : 605091
Vega : OTTHUMG00000014616
Target GenBank: BC023645

 

Reference Sequence Alignment

  

HsCD00045629      ATGAAGGGGGCAAGTGAGGAGAAGCTGGCATCTGTGTCCAACCTGGTCACTGTGTTTGAG
NM_173558.3       ATGAAGGGGGCAAGTGAGGAGAAGCTGGCATCTGTGTCCAACCTGGTCACTGTGTTTGAG
                  ************************************************************

HsCD00045629      AATAGCAGGACCCCAGAAGCAGCACCCAGAGGCCACAGGCTAGAGGACGTGCATCACCGC
NM_173558.3       AATAGCAGGACCCCAGAAGCAGCACCCAGAGGCCAGAGGCTAGAGGACGTGCATCACCGC
                  *********************************** ************************

HsCD00045629      CCTGAGTGCAGGCCTCCCGAGTCCCCAGGACCACGGGAGAAGACGAATGTCGGGGAGGCC
NM_173558.3       CCTGAGTGCAGGCCTCCCGAGTCCCCAGGACCACGGGAGAAGACGAATGTCGGGGAGGCC
                  ************************************************************

HsCD00045629      GTGGGGTCTGAGCCCAGGACAGTCAGCAGGAGGTACCTGAACTCCCTGAAGAACAAGCTG
NM_173558.3       GTGGGGTCTGAGCCCAGGACAGTCAGCAGGAGGTACCTGAACTCCCTGAAGAACAAGCTG
                  ************************************************************

HsCD00045629      TCCAGCGAAGCCTGGAGGAAATCTTGCCAGCCTGTGACCCTCTCAGGATCGGGGACGCAG
NM_173558.3       TCCAGCGAAGCCTGGAGGAAATCTTGCCAGCCTGTGACCCTCTCAGGATCGGGGACGCAG
                  ************************************************************

HsCD00045629      GAGCCAGAGAAGAAGATCGTCCAGGAGCTGCTGGAGACAGAGCAGGCCTATGTGGCGCGC
NM_173558.3       GAGCCAGAGAAGAAGATCGTCCAGGAGCTGCTGGAGACAGAGCAGGCCTATGTGGCGCGC
                  ************************************************************

HsCD00045629      CTCCACCTGCTAGACCAGGTGTTTTTCCAGGAGCTGCTGAAGACAGCCCGCAGCAGCAAG
NM_173558.3       CTCCACCTGCTAGACCAGGTGTTTTTCCAGGAGCTGCTGAAGACAGCCCGCAGCAGCAAG
                  ************************************************************

HsCD00045629      GCCTTCCCAGAGGATGTGGTCAGGGTCATCTTCTCCAACATCTCCTCCATCTATCAGTTC
NM_173558.3       GCCTTCCCAGAGGATGTGGTCAGGGTCATCTTCTCCAACATCTCCTCCATCTATCAGTTC
                  ************************************************************

HsCD00045629      CATTCTCAGTTCTTCCTCCCAGAGCTGCAGCGGCGCCTGGACGACTGGACAGCTAACCCC
NM_173558.3       CATTCTCAGTTCTTCCTCCCAGAGCTGCAGCGGCGCCTGGACGACTGGACAGCTAACCCC
                  ************************************************************

HsCD00045629      CGCATCGGTGACGTGATCCAGAAGCTGGCCCCCTTCCTGAAGATGTACAGTGAGTATGTC
NM_173558.3       CGCATCGGTGACGTGATCCAGAAGCTGGCCCCCTTCCTGAAGATGTACAGTGAGTATGTC
                  ************************************************************

HsCD00045629      AAGAACTTTGAGCGAGCGGCTGAGCTGCTGGCCACCTGGACCGACAAGTCTCCACTCTTC
NM_173558.3       AAGAACTTTGAGCGAGCGGCTGAGCTGCTGGCCACCTGGACCGACAAGTCTCCACTCTTC
                  ************************************************************

HsCD00045629      CAGGAGGTTCTCACTCGCATCCAGAGCAGCGAGGCTTCGGGCAGCCTGACCCTGCAGCAC
NM_173558.3       CAGGAGGTTCTCACTCGCATCCAGAGCAGCGAGGCTTCGGGCAGCCTGACCCTGCAGCAC
                  ************************************************************

HsCD00045629      CACATGCTGGAACCAGTGCAGAGAATTCCACGTTACGAGCTGCTGCTCAAGGAGTACATC
NM_173558.3       CACATGCTGGAACCAGTGCAGAGAATTCCACGTTACGAGCTGCTGCTCAAGGAGTACATC
                  ************************************************************

HsCD00045629      CAGAAGCTGCCAGCCCAGGCCCCAGACCAGGCCGATGCCCAGAAAGCCCTGGACATGATC
NM_173558.3       CAGAAGCTGCCAGCCCAGGCCCCAGACCAGGCCGATGCCCAGAAAGCCCTGGACATGATC
                  ************************************************************

HsCD00045629      TTCTCAGCTGCCCAGCACTCCAATGCAGCCATCACTGAGAGGGAGCGGCTGCAGGACCTG
NM_173558.3       TTCTCAGCTGCCCAGCACTCCAATGCAGCCATCACTGAGATGGAGCGGCTGCAGGACCTG
                  **************************************** *******************

HsCD00045629      TGGGAGGTGTACCAGCGCCTGGGCCTCGAGGACGACATAGTAGACCCCTCTAACACCCTG
NM_173558.3       TGGGAGGTGTACCAGCGCCTGGGCCTCGAGGACGACATAGTAGACCCCTCTAACACCCTG
                  ************************************************************

HsCD00045629      CTCCGTGAGGGCCCGGTCCTCAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTAC
NM_173558.3       CTCCGTGAGGGCCCGGTCCTCAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTAC
                  ************************************************************

HsCD00045629      CTTTTCTTGTTCAACAACATGCTGCTCTACTGTGTGCCCAGGGTGATCCAGGTGGGCGCC
NM_173558.3       CTTTTCTTGTTCAACAACATGCTGCTCTACTGTGTGCCCAGGGTGATCCAGGTGGGCGCC
                  ************************************************************

HsCD00045629      CAGTTCCAGGTGAGGACCCGCATCGATGTGGCCGGGATGAAGGTGCGGGAGCTGATGGAT
NM_173558.3       CAGTTCCAGGTGAGGACCCGCATCGATGTGGCCGGGATGAAGGTGCGGGAGCTGATGGAT
                  ************************************************************

HsCD00045629      GCTGAGTTTCCCCACTCCTTCCTGGTGTCCGGGAAGCAGCGCACCCTGGAGCTGCAAGCC
NM_173558.3       GCTGAGTTTCCCCACTCCTTCCTGGTGTCCGGGAAGCAGCGCACCCTGGAGCTGCAAGCC
                  ************************************************************

HsCD00045629      CGGTCCCAGGAGGAAATGATTTCCTGGATGCAGGCCTTCCAAGCAGCCATTGACCAAATC
NM_173558.3       CGGTCCCAGGAGGAAATGATTTCCTGGATGCAGGCCTTCCAAGCAGCCATTGACCAAATC
                  ************************************************************

HsCD00045629      GAGAAGCGGAATGAAACCTTCAAGGCTGCGGCCCAGGGGCCTGAGGGAGACATCCAGGAG
NM_173558.3       GAGAAGCGGAATGAAACCTTCAAGGCTGCGGCCCAGGGGCCTGAGGGAGACATCCAGGAG
                  ************************************************************

HsCD00045629      CAGGAGCTGCAGTCTGAGGAGCTGGGCCTCCGGGCACCGCAGTGGGTCCGGGACAAGATG
NM_173558.3       CAGGAGCTGCAGTCTGAGGAGCTGGGCCTCCGGGCACCGCAGTGGGTCCGGGACAAGATG
                  ************************************************************

HsCD00045629      GTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAACGCTCTGACGCGCCGTCGCCACCAC
NM_173558.3       GTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAACGCTCTGACGCGCCGTCGCCACCAC
                  ************************************************************

HsCD00045629      TGCCGGGCCTGCGGCTATGTGGTGTGTGCCAGGTGCTCCGACTACCGGGCCGAACTGAAA
NM_173558.3       TGCCGGGCCTGCGGCTATGTGGTGTGTGCCAGGTGCTCCGACTACCGGGCCGAACTGAAA
                  ************************************************************

HsCD00045629      TACGACGACAACAGGCCCAACCGAGTCTGCCTCCACTGCTACGCATTCCTCACTGGAAAT
NM_173558.3       TACGACGACAACAGGCCCAACCGAGTCTGCCTCCACTGCTACGCATTCCTCACTGGAAAT
                  ************************************************************

HsCD00045629      GTGCTGCCTGAGGCCAAGGAGGACAAGAGGCGGGGCATCCTGGAGAAAGGGTCCTCAGCC
NM_173558.3       GTGCTGCCTGAGGCCAAGGAGGACAAGAGGCGGGGCATCCTGGAGAAAGGGTCCTCAGCC
                  ************************************************************

HsCD00045629      ACGCCTGACCAGAGCCTGATGTGCAGCTTCCTGCAGCTCATCGGGGACAAGTGGGGCAAG
NM_173558.3       ACGCCTGACCAGAGCCTGATGTGCAGCTTCCTGCAGCTCATCGGGGACAAGTGGGGCAAG
                  ************************************************************

HsCD00045629      AGCGGCCCCCGGGGCTGGTGTGTGATCCCTCGGGATGACCCCCTCGTGCTCTATGTCTAT
NM_173558.3       AGCGGCCCCCGGGGCTGGTGTGTGATCCCTCGGGATGACCCCCTCGTGCTCTATGTCTAT
                  ************************************************************

HsCD00045629      GCTGCCCCTCAGGACATGAGGGCTCACACCTCCATCCCCCTGCTGGGCTACCAGGTGACT
NM_173558.3       GCTGCCCCTCAGGACATGAGGGCTCACACCTCCATCCCCCTGCTGGGCTACCAGGTGACT
                  ************************************************************

HsCD00045629      GTTGGGCCCCAGGGGGACCCTCGGGTCTTCCAGCTACAGCAGTCAGGCCAGCTCTACACC
NM_173558.3       GTTGGGCCCCAGGGGGACCCTCGGGTCTTCCAGCTACAGCAGTCAGGCCAGCTCTACACC
                  ************************************************************

HsCD00045629      TTCAAGGCCGAGACGGAGGAGCTGAAGGGCCGCTGGGTGAAGGCCATGGAGCGGGCGGCC
NM_173558.3       TTCAAGGCCGAGACGGAGGAGCTGAAGGGCCGCTGGGTGAAGGCCATGGAGCGGGCGGCC
                  ************************************************************

HsCD00045629      AGTGGCTGGAGCCCCAGCTGGCCCAACGATGGGGACCTGTCCGACTAG
NM_173558.3       AGTGGCTGGAGCCCCAGCTGGCCCAACGATGGGGACCTGTCCGACTGA
                  **********************************************..

Pathways

Reactome : Cell death signalling via NRAGE, NRIF and NADE
Reactome : G alpha (12/13) signalling events
Reactome : GPCR downstream signaling
Reactome : NRAGE signals death through JNK
Reactome : Rho GTPase cycle
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : Signaling by Rho GTPases
Reactome : Signalling by NGF
Reactome : p75 NTR receptor-mediated signalling

Clone

Cloning Information : 182471
HIP Master Clone ID : 157415
Original Clone ID : FLH182471.01X

Organism


TAX_ID : 9606
Species Specific ID: 221472

Genome

Chromosome : 6
Map Location : 6p21.2
Ensembl : ENSG00000146192

Reagents

Labome : FGD2-antibody