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PPP2R4 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP2R4
Gene Name:  protein phosphatase 2A activator, regulatory subunit 4
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH019843.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000494

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 972nts         Open reading frame : 1 to 972
Reference Sequence Alignment

ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAGTCGCTCTT
CTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTT
GGGAATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTG
GCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAG
GAGTCAGTGGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCT
GCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATT
GTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGG
ATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTC
ATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGAT
GAGAAGGCCGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATT
ACCGAGATGAAGACTGGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCC
GTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTG
GAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTC
ACGTCGGGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1161
Start on reference sequence 190
Mutations:
None
Discrepancy:
c232g,L78V; g843c
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP2R4
Symbol Nomenclature : PPP2R4
SYNONYM : PP2A; PR53; PTPA
Designation : PP2A phosphatase activator|PP2A subunit B' isoform PR53|phosphotyrosyl phosphatase activator|protein phosphatase 2A, regulatory subunit B' (PR 53)|serine/threonine-protein phosphatase 2A activator|serine/threonine-protein phosphatase 2A regulatory subunit B'
Full Nomenclature : protein phosphatase 2A activator, regulatory subunit 4
GENEID : 5524
Locus Tag : RP11-247A12.4
GI : 61365904
GenBank Accession : AY890844
HGNC : 9308
MIM : 600756
Vega : OTTHUMG00000020774
Target GenBank: NM_021131

 

Reference Sequence Alignment

  

HsCD00000494        ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
NM_178000.2         ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
NM_178001.2         ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
NM_021131.4         ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
NM_001271832.1      ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACT
                    ************************************************************

HsCD00000494        CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
NM_178000.2         CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
NM_178001.2         CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
NM_021131.4         CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
NM_001271832.1      CAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAG
                    ************************************************************

HsCD00000494        CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
NM_178000.2         CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
NM_178001.2         CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
NM_021131.4         CGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAG
NM_001271832.1      CGTTCTCAG---------------------------------------------------
                    *********                                                   

HsCD00000494        GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAG------------------------
NM_178000.2         GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAG------------------------
NM_178001.2         GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGATGTGGAATGAGGTTCATGAGGAA
NM_021131.4         GGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGA-------------------------
NM_001271832.1      ------------------------------------------------------------
                                                                                

HsCD00000494        ------------------------------------------------------------
NM_178000.2         ------------------------------------------------------------
NM_178001.2         AAGGAGCAGGCTGCAAAGCAGAGTGTGTCCTGCGATGAATGCATACCATTACCCCGCGCC
NM_021131.4         ------------------------------------------------------------
NM_001271832.1      ------------------------------------------------------------
                                                                                

HsCD00000494        ---------------------GCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGAC
NM_178000.2         ---------------------GCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGAC
NM_178001.2         GGGCACTGTGCACCTTCGGAGGCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGAC
NM_021131.4         --------------------GGCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGAC
NM_001271832.1      ---------------------GCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGAC
                                         ***************************************

HsCD00000494        AGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATAC
NM_178000.2         AGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATAC
NM_178001.2         AGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATAC
NM_021131.4         AGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATAC
NM_001271832.1      AGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATAC
                    ************************************************************

HsCD00000494        AGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCT
NM_178000.2         AGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCT
NM_178001.2         AGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCT
NM_021131.4         AGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCT
NM_001271832.1      AGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCT
                    ************************************************************

HsCD00000494        ACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAAC
NM_178000.2         ACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAAC
NM_178001.2         ACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAAC
NM_021131.4         ACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAAC
NM_001271832.1      ACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAAC
                    ************************************************************

HsCD00000494        TCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGT
NM_178000.2         TCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGT
NM_178001.2         TCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGT
NM_021131.4         TCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGT
NM_001271832.1      TCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGT
                    ************************************************************

HsCD00000494        CTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTC
NM_178000.2         CTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTC
NM_178001.2         CTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTC
NM_021131.4         CTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTC
NM_001271832.1      CTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTC
                    ************************************************************

HsCD00000494        AATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGC
NM_178000.2         AATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGC
NM_178001.2         AATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGC
NM_021131.4         AATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGC
NM_001271832.1      AATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGC
                    ************************************************************

HsCD00000494        AGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCG
NM_178000.2         AGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCG
NM_178001.2         AGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCG
NM_021131.4         AGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCG
NM_001271832.1      AGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCG
                    ************************************************************

HsCD00000494        CAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAAT
NM_178000.2         CAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAAT
NM_178001.2         CAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAAT
NM_021131.4         CAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAAT
NM_001271832.1      CAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGAAT
                    ************************************************************

HsCD00000494        GAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACT
NM_178000.2         GAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACT
NM_178001.2         GAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACT
NM_021131.4         GAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACT
NM_001271832.1      GAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACT
                    ************************************************************

HsCD00000494        GGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCC
NM_178000.2         GGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCC
NM_178001.2         GGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCC
NM_021131.4         GGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCC
NM_001271832.1      GGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCC
                    ************************************************************

HsCD00000494        AAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTG
NM_178000.2         AAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTG
NM_178001.2         AAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTG
NM_021131.4         AAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTG
NM_001271832.1      AAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTG
                    ************************************************************

HsCD00000494        ATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTTG
NM_178000.2         ATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAG
NM_178001.2         ATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAG
NM_021131.4         ATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAG
NM_001271832.1      ATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAG
                    *******************************************************:*

Pathways

NCI : Validated targets of C-MYC transcriptional repression
NCI : p53 pathway
WikiPathway : Epithelium TarBase
WikiPathway : Glycogen Metabolism
WikiPathway : Leukocyte TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase
WikiPathway : Wnt Signaling Pathway and Pluripotency

Protein

UniProt : Q15257
HPRD : 02858

Clone

Cloning Information : 19843
HIP Master Clone ID : 34353
Original Clone ID : FLH019843.01L

Organism


TAX_ID : 9606
Species Specific ID: 5524

Genome

Chromosome : 9
Map Location : 9q34
Ensembl : ENSG00000119383

Reagents

Labome : PPP2R4-antibody