DNASU Plasmid Repository • 480.965.5697 | Email

UBE2W (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  UBE2W
Gene Name:  ubiquitin-conjugating enzyme E2W (putative)
Sequence             
Original Clone ID: ccsbBroadEn_03571
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC016326.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00505222

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 454nts         Open reading frame : 1 to 454
Reference Sequence Alignment

ATGGCGTCAATGCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
AAAACAAAATGGTGGTATCATGATGATACTTGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2W
Symbol Nomenclature : UBE2W
SYNONYM : UBC-16; UBC16
Designation : N-terminus-conjugating E2|ubiquitin carrier protein W|ubiquitin-conjugating enzyme 16|ubiquitin-conjugating enzyme 2W|ubiquitin-conjugating enzyme E2 W|ubiquitin-protein ligase W
Full Nomenclature : ubiquitin-conjugating enzyme E2W (putative)
GENEID : 55284
GenBank Accession : EU446497
HGNC : 25616
MIM : 614277
Vega : OTTHUMG00000164517
Target GenBank: EU446497

 

Reference Sequence Alignment

  

HsCD00505222        ------------------------------------------------------------
NM_001271015.1      ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
NM_001001481.2      ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
NM_018299.4         ATGTTGAGCCCCCGCGGCGTGACGCGAGCACGGCAGCTCCTCCCCCTGCGTCTCTGGCCT
                                                                                

HsCD00505222        ------------------------------------------------------------
NM_001271015.1      CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAATGCAG------------------
NM_001001481.2      CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAATGCAGACCACAGGAAGGAGGGTG
NM_018299.4         CGCCGGTCTTGGGGGGATGGTTCCATCATGGCGTCAATGCAG------------------
                                                                                

HsCD00505222        ATGGCGTCAATGCAGAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
NM_001271015.1      ---------------AAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
NM_001001481.2      GAAGTATGGTTTCCAAAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
NM_018299.4         ---------------AAACGACTACAGAAAGAACTGTTGGCTTTGCAAAATGACCCACCT
                                   *********************************************

HsCD00505222        CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_001271015.1      CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_001001481.2      CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
NM_018299.4         CCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACAGTGGATTGTAGAC
                    ************************************************************

HsCD00505222        ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_001271015.1      ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_001001481.2      ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
NM_018299.4         ATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAATTT
                    ************************************************************

HsCD00505222        AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_001271015.1      AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_001001481.2      AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
NM_018299.4         AGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTT
                    ************************************************************

HsCD00505222        CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_001271015.1      CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_001001481.2      CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
NM_018299.4         CATCCTCATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCC
                    ************************************************************

HsCD00505222        CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_001271015.1      CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_001001481.2      CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
NM_018299.4         CCAGCGCTCTCAGTCCAATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAG
                    ************************************************************

HsCD00505222        GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_001271015.1      GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_001001481.2      GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
NM_018299.4         GAAAAGAGACGACCACCGGATAATTCTTTTTATGTGCGAACATGTAACAAGAATCCAAAG
                    ************************************************************

HsCD00505222        AAAACAAAATGGTGGTATCATGATGATACTTGT---------------------------
NM_001271015.1      AAAACAAAATGGTGGTATCATGAACTGAAATCTGCCTTCATACTATCTATTACGGATTGA
NM_001001481.2      AAAACAAAATGGTGGTATCATGATGATACTTGTTGA------------------------
NM_018299.4         AAAACAAAATGGTGGTATCATGATGATACTTGTTGA------------------------
                    ***********************: : *.:* *                           

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Cloning Information : 667607
Original Clone ID : ccsbBroadEn_03571

Organism


TAX_ID : 9606
Species Specific ID: 55284

Genome

Chromosome : 8
Map Location : 8q21.11
Ensembl : ENSG00000104343

Reagents

Labome : UBE2W-antibody