DNASU Plasmid Repository • 480.965.5697 | Email

UBE2D4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  UBE2D4
Gene Name:  ubiquitin-conjugating enzyme E2D 4 (putative)
Sequence             
Original Clone ID: ccsbBroadEn_03347
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC004104.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00505243

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 442nts         Open reading frame : 1 to 442
Reference Sequence Alignment

ATGGCGCTAAAGCGGATCCAGAAGGAATTAACCGACTTGCAGAGGGATCCTCCTGCCCAG
TGTTCTGCAGGACCTGTCGGTGATGACTTGTTCCACTGGCAGGCCACCATCATGGGCCCG
AATGACAGTCCTTACCAAGGAGGTGTTTTCTTCCTGACCATCCACTTTCCTACAGATTAC
CCGTTCAAGCCCCCAAAGGTTGCTTTCACAACCAAAATTTATCACCCTAATATCAACAGC
AATGGCAGCATCTGCCTTGATATCCTGCGGTCTCAGTGGTCTCCAGCGTTGACTGTGTCA
AAAGTTCTCTTGTCCATCTGCTCGCTGCTCTGCGACCCCAACCCCGATGACCCCCTGGTG
CCAGAGATAGCACACACCTACAAGGCCGACAGAGAGAAGTACAACAGACTAGCAAGAGAG
TGGACACAAAAATATGCTATG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBE2D4
Symbol Nomenclature : UBE2D4
SYNONYM : HBUCE1
Designation : ubiquitin carrier protein D4|ubiquitin-conjugating enzyme E2 D4|ubiquitin-conjugating enzyme HBUCE1|ubiquitin-protein ligase D4
Full Nomenclature : ubiquitin-conjugating enzyme E2D 4 (putative)
GENEID : 51619
GenBank Accession : HQ447284
HGNC : 21647
Vega : OTTHUMG00000128971
Target GenBank: HQ447284

 

Reference Sequence Alignment

  

HsCD00505243      ------ATGGCGCTAAAGCGGATCCAGAAGGAATTAACCGACTTGCAGAGGGATCCTCCT
NM_181888.3       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181893.2       ATGCTTTCTAACCGAAAGTGCCTTTCAAAAGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181891.2       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_003339.2       ------ATGGCTCTGAAGAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCA
NM_181890.2       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181887.2       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181892.3       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181886.3       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_003340.6       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_181889.2       ------ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCA
NM_003338.4       ------ATGGCGCTGAAGAGGATTCAGAAAGAATTGAGTGATCTACAGCGCGATCCACCT
NM_015983.3       ------ATGGCGCTAAAGCGGATCCAGAAGGAATTAACCGACTTGCAGAGGGATCCTCCT
                        :  .. * .**. * .*  . **.*** * *  **  *. . .* ** **:**:

HsCD00505243      GCCCAGTGTTCTGCAGGACCTGTCGGTGATGACTTGTTCCACTGGCAGGCCACCATCATG
NM_181888.3       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181893.2       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181891.2       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_003339.2       GCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATG
NM_181890.2       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181887.2       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181892.3       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181886.3       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_003340.6       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_181889.2       GCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATG
NM_003338.4       GCTCACTGTTCAGCTGGACCTGTGGGAGATGACTTGTTCCACTGGCAAGCCACTATTATG
NM_015983.3       GCCCAGTGTTCTGCAGGACCTGTCGGTGATGACTTGTTCCACTGGCAGGCCACCATCATG
                  ** ** *****:**:**:**:** ** ***** :**** ** *****.** ** ** ***

HsCD00505243      GGCCCGAATGACAGTCCTTACCAAGGAGGTGTTTTCTTCCTGACCATCCACTTTCCTACA
NM_181888.3       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181893.2       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181891.2       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_003339.2       GGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACA
NM_181890.2       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181887.2       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181892.3       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181886.3       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_003340.6       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_181889.2       GGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCCTACA
NM_003338.4       GGGCCTCCTGATAGCGCATATCAAGGTGGAGTCTTCTTTCTCACTGTACATTTTCCGACA
NM_015983.3       GGCCCGAATGACAGTCCTTACCAAGGAGGTGTTTTCTTCCTGACCATCCACTTTCCTACA
                  ** ** ..*** **  * ** **.** **:** ** **  * ** .* ** ** ** ***

HsCD00505243      GATTACCCGTTCAAGCCCCCAAAGGTTGCTTTCACAACCAAAATTTATCACCCTAATATC
NM_181888.3       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181893.2       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181891.2       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_003339.2       GATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181890.2       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181887.2       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181892.3       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181886.3       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_003340.6       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_181889.2       GACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATT
NM_003338.4       GATTATCCTTTTAAACCACCAAAGATTGCTTTCACAACAAAAATTTACCATCCAAACATA
NM_015983.3       GATTACCCGTTCAAGCCCCCAAAGGTTGCTTTCACAACCAAAATTTATCACCCTAATATC
                  ** ** ** ** **.**.**:***.****:** *****.*.****** ** **:** ** 

HsCD00505243      AACAGCAATGGCAGCATCTGCCTTGATATCCTGCGGTCTCAGTGGTCTCCAGCGTTGACT
NM_181888.3       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181893.2       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181891.2       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_003339.2       AACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACT
NM_181890.2       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181887.2       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181892.3       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181886.3       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_003340.6       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_181889.2       AACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTTAACA
NM_003338.4       AACAGTAATGGAAGTATTTGTCTCGATATTCTGAGGTCACAATGGTCACCAGCTCTGACT
NM_015983.3       AACAGCAATGGCAGCATCTGCCTTGATATCCTGCGGTCTCAGTGGTCTCCAGCGTTGACT
                  ***** *****.** ** ** ** ***** **..*.**:**.***** **:**  *.**:

HsCD00505243      GTGTCAAAAGTTCTCTTGTCCATCTGCTCGCTGCTCTGCGACCCCAACCCCGATGACCCC
NM_181888.3       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181893.2       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181891.2       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_003339.2       ATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCT
NM_181890.2       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181887.2       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181892.3       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181886.3       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_003340.6       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_181889.2       ATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGACCCC
NM_003338.4       GTATCAAAAGTTTTATTGTCCATATGTTCTCTACTTTGTGATCCTAATCCAGATGACCCC
NM_015983.3       GTGTCAAAAGTTCTCTTGTCCATCTGCTCGCTGCTCTGCGACCCCAACCCCGATGACCCC
                  .* **:*****: * **.***** ** ** **. * ** ** ** ** **.***** ** 

HsCD00505243      CTGGTGCCAGAGATAGCACACACCTACAAGGCCGACAGAGAGAAGTACAACAGACTAGCA
NM_181888.3       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_181893.2       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_181891.2       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_003339.2       TTAGTGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAATAGCT
NM_181890.2       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_181887.2       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_181892.3       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAATAGGTTAGCA
NM_181886.3       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_003340.6       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_181889.2       CTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAACAGAATATCT
NM_003338.4       TTAGTACCAGATATTGCACAAATCTATAAATCAGACAAAGAAAAATACAACAGACATGCA
NM_015983.3       CTGGTGCCAGAGATAGCACACACCTACAAGGCCGACAGAGAGAAGTACAACAGACTAGCA
                   *.**.**:** **:**:*. * *** **. *.** *.*** **.***** **. :: *:

HsCD00505243      AGAGAGTGGACACAAAAATATGCTATG------
NM_181888.3       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_181893.2       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_181891.2       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_003339.2       CGGGAATGGACTCAGAAGTATGCGATGTAA---
NM_181890.2       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_181887.2       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_181892.3       AGAGAGTGGACAGAGAAATACGCTATGTTGTAG
NM_181886.3       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_003340.6       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_181889.2       CGGGAATGGACTCAGAAGTATGCCATGTGA---
NM_003338.4       AGAGAATGGACTCAGAAATATGCAATGTAA---
NM_015983.3       AGAGAGTGGACACAAAAATATGCTATGTAA---
                  .*.**.*****: *.**.** ** ***      

Pathways

Panther : Ubiquitin proteasome pathway
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Clone

Cloning Information : 605312
Original Clone ID : ccsbBroadEn_03347

Organism


TAX_ID : 9606
Species Specific ID: 51619

Genome

Chromosome : 7
Map Location : 7p13
Ensembl : ENSG00000078967

Reagents

Labome : UBE2D4-antibody