DNASU Plasmid Repository • 480.965.5697 | Email

PSD2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  PSD2
Gene Name:  pleckstrin and Sec7 domain containing 2
Sequence             
Original Clone ID: ccsbBroadEn_09163
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC038233.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00505671

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2314nts         Open reading frame : 1 to 2314
Reference Sequence Alignment

ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
GGGGACATGGCGTTTGAGGGGGACATGGGGGCGGCTGGTGGTGATGGGGAGCTGGGCAGC
CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
AAAACCACAAAGGATGCCACTGGGCCTGATACT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PSD2
Symbol Nomenclature : PSD2
Designation : 6330404E20Rik|PH and SEC7 domain-containing protein 2|pleckstrin homology and SEC7 domain-containing protein 2
Full Nomenclature : pleckstrin and Sec7 domain containing 2
GENEID : 84249
GenBank Accession : AM393868
HGNC : 19092
Vega : OTTHUMG00000129240
Target GenBank: AM393868

 

Reference Sequence Alignment

  

HsCD00505671      ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
NM_032289.2       ATGGAGGAGGACAAGCTCTTATCTGCAGTGCCTGAGGAAGGCGATGCCACCCGTGACCCC
                  ************************************************************

HsCD00505671      GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
NM_032289.2       GGTCCAGAGCCTGAAGAGGAGCCAGGGGTCCGGAATGGGATGGCCAGTGAGGGCCTGAAC
                  ************************************************************

HsCD00505671      AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
NM_032289.2       AGCAGCCTCTGCAGCCCAGGGCACGAGCGAAGGGGCACCCCAGCGGACACTGAGGAACCC
                  ************************************************************

HsCD00505671      ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
NM_032289.2       ACGAAGGACCCAGATGTGGCCTTCCATGGCCTCAGCCTTGGCCTCTCTCTCACCAATGGC
                  ************************************************************

HsCD00505671      CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
NM_032289.2       CTAGCCCTGGGGCCAGACTTGAACATTCTGGAAGATTCAGCGGAGTCCAGGCCCTGGAGG
                  ************************************************************

HsCD00505671      GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
NM_032289.2       GCTGGCGTGCTGGCAGAGGGGGACAATGCTTCCAGGAGCCTCTACCCAGATGCTGAGGAC
                  ************************************************************

HsCD00505671      CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
NM_032289.2       CCTCAGCTGGGGTTGGATGGTCCCGGGGAGCCAGATGTGCGGGATGGCTTCAGCGCCACG
                  ************************************************************

HsCD00505671      TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
NM_032289.2       TTTGAGAAGATTCTGGAGTCAGAGCTGCTGCGGGGCACCCAGTACAGCAGCCTCGACTCC
                  ************************************************************

HsCD00505671      CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
NM_032289.2       CTAGACGGGCTGAGCCTCACGGATGAGAGCGACAGCTGCGTCAGCTTCGAGGCCCCCCTC
                  ************************************************************

HsCD00505671      ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
NM_032289.2       ACACCCCTCATCCAGCAGCGGGCCCGTGACAGCCCTGAGCCAGGGGCTGGGTTGGGCATT
                  ************************************************************

HsCD00505671      GGGGACATGGCGTTTGAGGGGGACATGGGGGCGGCTGGTGGTGATGGGGAGCTGGGCAGC
NM_032289.2       GGGGACATGGCGTTTGAGGGGGACATGGGGGCAGCTGGTGGTGATGGGGAGCTGGGCAGC
                  ********************************.***************************

HsCD00505671      CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
NM_032289.2       CCCCTGCGGCGCTCCATCTCCAGCAGCCGCTCTGAGAATGTCCTGAGCCGCCTGTCTCTC
                  ************************************************************

HsCD00505671      ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
NM_032289.2       ATGGCCATGCCCAATGGATTCCATGAAGATGGCCCTCAGGGCCCAGGGGGGGATGAGGAT
                  ************************************************************

HsCD00505671      GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
NM_032289.2       GATGATGAGGAGGACACGGACAAGTTGCTGAACTCAGCCAGTGACCCCAGCCTGAAGGAT
                  ************************************************************

HsCD00505671      GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
NM_032289.2       GGCCTGTCAGACTCAGACTCTGAGCTCAGCAGCTCGGAGGGGTTGGAGCCTGGTAGTGCA
                  ************************************************************

HsCD00505671      GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
NM_032289.2       GACCCTCTGGCCAACGGGTGCCAGGGGGTCAGTGAAGCTGCTCATCGGCTGGCACGCCGT
                  ************************************************************

HsCD00505671      CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
NM_032289.2       CTCTACCACCTCGAGGGCTTCCAGCGCTGTGATGTGGCCCGGCAGCTGGGCAAGAACAAC
                  ************************************************************

HsCD00505671      GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
NM_032289.2       GAGTTTAGCAGGCTGGTGGCCGGGGAGTACCTCAGTTTCTTCGACTTCTCGGGCTTGACT
                  ************************************************************

HsCD00505671      CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
NM_032289.2       CTGGACGGAGCACTCAGAACATTCTTGAAGGCCTTCCCGCTGATGGGGGAGACACAAGAG
                  ************************************************************

HsCD00505671      CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
NM_032289.2       CGTGAGCGGGTCCTCACACACTTCTCCCGCCGGTACTGCCAGTGCAACCCTGATGACAGC
                  ************************************************************

HsCD00505671      ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
NM_032289.2       ACTTCGGAAGATGGGATCCACACGCTCACCTGTGCCCTGATGCTGCTCAACACGGACCTG
                  ************************************************************

HsCD00505671      CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
NM_032289.2       CACGGCCACAACATTGGCAAAAAGATGTCCTGTCAGCAATTCATTGCCAACTTGGACCAG
                  ************************************************************

HsCD00505671      CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
NM_032289.2       CTGAATGATGGCCAAGACTTTGCCAAAGACCTGCTGAAGACCCTTTACAACTCCATCAAG
                  ************************************************************

HsCD00505671      AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
NM_032289.2       AATGAAAAGCTGGAATGGGCCATTGATGAGGATGAGCTGAGGAAATCCCTGTCTGAGCTG
                  ************************************************************

HsCD00505671      GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
NM_032289.2       GTGGATGACAAGTTCGGGACAGGCACGAAGAAGGTGACGCGAATCCTGGATGGTGGCAAC
                  ************************************************************

HsCD00505671      CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
NM_032289.2       CCCTTCCTGGATGTCCCACAGGCGCTCAGTGCCACCACCTACAAGCACGGCGTCCTGACC
                  ************************************************************

HsCD00505671      CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
NM_032289.2       CGGAAGACTCACGCTGACATGGATGGCAAGAGGACGCCCCGTGGGAGGCGTGGCTGGAAG
                  ************************************************************

HsCD00505671      AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
NM_032289.2       AAATTCTACGCAGTGCTCAAAGGGACCATCCTGTACCTGCAGAAGGATGAGTACAGGCCT
                  ************************************************************

HsCD00505671      GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
NM_032289.2       GACAAAGCTCTATCGGAGGGTGACCTGAAGAACGCCATTCGCGTGCATCACGCTCTGGCC
                  ************************************************************

HsCD00505671      ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
NM_032289.2       ACCAGGGCCTCTGACTACAGCAAGAAGTCCAACGTGCTGAAGCTTAAGACAGCCGACTGG
                  ************************************************************

HsCD00505671      AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
NM_032289.2       AGGGTATTCCTCTTCCAGGCACCGAGCAAGGAAGAAATGCTGTCCTGGATCCTCAGGATC
                  ************************************************************

HsCD00505671      AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
NM_032289.2       AACCTGGTGGCAGCCATCTTCTCTGCCCCGGCCTTCCCAGCCGCTGTCAGCTCCATGAAG
                  ************************************************************

HsCD00505671      AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
NM_032289.2       AAGTTCTGTCGGCCCCTGCTGCCCTCCTGCACCACCCGCCTCTGCCAGGAGGAGCAACTG
                  ************************************************************

HsCD00505671      CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
NM_032289.2       CGGTCTCATGAGAATAAGTTGAGGCAGCTGACTGCGGAGCTGGCCGAACACAGGTGTCAC
                  ************************************************************

HsCD00505671      CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
NM_032289.2       CCAGTCGAGAGGGGCATCAAGTCCAAGGAGGCCGAGGAGTACCGGTTGAAGGAGCACTAT
                  ************************************************************

HsCD00505671      CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
NM_032289.2       CTCACCTTCGAGAAAAGCCGTTATGAGACCTATATCCACCTCCTGGCTATGAAAATCAAA
                  ************************************************************

HsCD00505671      GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
NM_032289.2       GTGGGCTCAGATGATCTGGAGCGGATTGAGGCCCGGCTGGCCACTCTGGAAGGGGATGAC
                  ************************************************************

HsCD00505671      CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
NM_032289.2       CCTTCTCTCCGGAAGACACATTCAAGCCCTGCCCTCAGCCAGGGCCATGTGACTGGCAGC
                  ************************************************************

HsCD00505671      AAAACCACAAAGGATGCCACTGGGCCTGATACT---
NM_032289.2       AAAACCACAAAGGATGCCACTGGGCCTGATACTTAG
                  *********************************   

Protein

SMART domain : Sec7 : Sec7 domain
SMART domain : PH : Pleckstrin homology domain.
HPRD : 15190

Clone

Cloning Information : 614270
Original Clone ID : ccsbBroadEn_09163

Organism


TAX_ID : 9606
Species Specific ID: 84249

Genome

Chromosome : 5
Map Location : 5q31.2
Ensembl : ENSG00000146005

Reagents

Labome : PSD2-antibody