Gene:
Gene Symbol:
ANO4
Gene Name:
anoctamin 4
Original Clone ID:
ccsbBroadEn_13086
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC109308.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505944
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGGCAAGCTCTTCTGGAATCACTAATGGAAAAACCAAAGTCTTCCACCCAGAAGGA
GGTGTGGATTTGCAAGGCTACCAGCTGGATATGCAAATACTACCTGACGGGCCAAAGAGT
GATGTGGACTTTTCAGAGATTCTTAATGCAATACAAGAAATGGCCAAGGATGTCAATATT
CTTTTTGATGAATTAGAAGCTGTCAGCAGTCCTTGCAAAGATGACGATTCTCTTCTTCAC
CCTGGAAACCTGACTAGTACTTCAGATGATGCCAGCAGATTGGAAGCCGGGGGAGAGACA
GTGCCAGAAAGAAACAAATCAAATGGACTTTACTTTCGAGATGGAAAGTGTCGAATTGAC
TACATCCTTGTGTACAGAAAATCCAACCCCCAGACTGAAAAGAGAGAAGTATTTGAAAGA
AACATTAGAGCAGAAGGATTGCAAATGGAGAAAGAGTCCTCTCTAATAAATAGTGACATT
ATCTTTGTGAAGTTGCATGCCCCATGGGAAGTCCTTGGAAGATATGCAGAACAAATGAAT
GTAAGAATGCCTTTCAGGAGAAAAATCTATTACCTGCCCCGCCGTTACAAGTTCATGAGC
AGGATCGATAAACAAATAAGCAGGTTTCGGAGATGGTTACCTAAGAAGCCAATGAGGCTG
GACAAGGAGACACTGCCAGACCTGGAGGAGAATGACTGCTACACTGCCCCTTTCAGCCAG
CAAAGGATCCATCACTTCATCATACACAACAAAGAAACGTTCTTCAACAATGCCACAAGA
AGTAGAATCGTGCATCACATTTTACAAAGAATAAAATATGAAGAAGGAAAAAACAAGATT
GGTCTGAATCGTTTGCTTACCAATGGCTCCTATGAAGCTGCGTTTCCCCTGCATGAGGGA
AGTTATAGAAGTAAAAACTCCATTCGAACCCATGGAGCAGAAAACCACCGACATCTACTC
TATGAGTGCTGGGCCTCCTGGGGCGTGTGGTATAAATACCAACCTTTGGATCTTGTAAGG
CGGTACTTTGGAGAGAAGATTGGGTTATATTTTGCCTGGTTGGGCTGGTACACCGGCATG
CTCTTCCCAGCTGCCTTCATTGGATTGTTTGTCTTTTTGTATGGCGTCACCACTCTGGAT
CACAGCCAAGTCAGTAAAGAAGTCTGCCAAGCTACAGATATCATCATGTGTCCTGTGTGT
GATAAATACTGTCCATTCATGAGGCTGTCAGACAGCTGTGTATATGCCAAGGTAACCCAC
CTTTTTGACAATGGAGCCACTGTCTTCTTTGCTGTTTTCATGGCAGTCTGGGCAACAGTT
TTCCTGGAGTTTTGGAAAAGACGGCGAGCAGTAATTGCTTATGACTGGGATTTGATAGAC
TGGGAAGAAGAGGAGGAAGAAATACGACCCCAGTTTGAAGCCAAGTATTCCAAGAAAGAG
CGGATGAATCCAATTTCTGGAAAGCCAGAACCTTATCAAGCATTTACAGATAAATGCAGC
AGACTTATCGTTTCTGCATCTGGAATATTTTTTATGATCTGCGTGGTGATTGCTGCCGTG
TTCGGGATCGTCATTTACCGGGTGGTGACTGTCAGCACTTTCGCTGCCTTTAAGTGGGCG
TTAATCAGGAATAACTCTCAGGTTGCAACCACAGGGACTGCTGTGTGCATCAACTTCTGT
ATCATTATGTTGCTGAATGTGCTCTATGAAAAAGTTGCCCTGCTTCTGACGAATTTAGAA
CAGCCTCGCACAGAGTCTGAGTGGGAGAACAGCTTCACCCTGAAAATGTTTCTTTTTCAG
TTTGTCAATCTGAACAGCTCCACATTTTACATCGCATTCTTCCTCGGAAGATTTACAGGA
CACCCAGGTGCCTACTTGAGGCTGATAAACAGGTGGAGACTAGAAGAGTGCCACCCTAGT
GGATGCCTTATTGATCTGTGTATGCAAATGGGTATTATAATGGTGCTAAAGCAGACCTGG
AATAATTTCATGGAACTTGGCTACCCGTTAATTCAGAATTGGTGGACTAGAAGAAAAGTA
CGACAAGAACATGGACCTGAAAGGAAAATAAGTTTCCCACAATGGGAAAAGGACTATAAC
CTTCAGCCGATGAATGCCTATGGACTCTTCGATGAATACTTAGAAATGATTCTTCAGTTT
GGATTCACAACTATCTTTGTGGCAGCTTTTCCCCTAGCACCACTTCTGGCCTTACTGAAT
AACATAATTGAAATTCGACTTGATGCTTACAAATTTGTCACACAGTGGAGGAGACCTTTA
GCTTCAAGGGCCAAAGACATAGGAATTTGGTATGGAATTCTTGAAGGCATTGGAATTCTC
TCTGTTATCACAAATGCATTTGTCATAGCGATAACATCTGACTTTATCCCTCGCTTGGTG
TATGCTTATAAGTATGGACCTTGTGCAGGCCAAGGAGAAGCTGGGCAAAAGTGCATGGTT
GGCTATGTGAATGCCAGCTTGTCTGTATTTCGAATTTCTGACTTTGAGAACCGATCTGAG
CCTGAATCTGATGGCAGTGAGTTCTCGGGGACTCCTCTTAAGTACTGCAGATACCGGGAC
TACCGTGACCCGCCTCATTCACTGGTGCCCTATGGCTACACACTGCAGTTTTGGCATGTC
CTAGCTGCTCGATTAGCTTTTATCATTGTCTTTGAGCACCTCGTGTTTTGTATAAAGCAC
CTCATTTCATATCTGATCCCAGACCTCCCAAAAGACCTAAGGGATCGAATGAGAAGAGAG
AAGTACTTGATTCAGGAGATGATGTATGAAGCAGAACTGGAACGTCTCCAGAAGGAACGA
AAGGAGAGGAAGAAGAATGGAAAAGCACACCACAACGAGTGGCCG
Protein sequence predicted based on nucleotide sequence
MEASSSGITNGKTKVFHPEGGVDLQGYQLDMQILPDGPKSDVDFSEILNAIQEMAKDVNI LFDELEAVSSPCKDDDSLLHPGNLTSTSDDASRLEAGGETVPERNKSNGLYFRDGKCRID YILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLINSDIIFVKLHAPWEVLGRYAEQMN VRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQ QRIHHFIIHNKETFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEG SYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGM LFPAAFIGLFVFLYGVTTLDHSQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTH LFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKE RMNPISGKPEPYQAFTDKCSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWA LIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQ FVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTW NNFMELGYPLIQNWWTRRKVRQEHGPERKISFPQWEKDYNLQPMNAYGLFDEYLEMILQF GFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGIL SVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSE PESDGSEFSGTPLKYCRYRDYRDPPHSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKH LISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
