Gene:
Gene Symbol:
AP4E1
Gene Name:
adaptor-related protein complex 4, epsilon 1 subunit
Original Clone ID:
ccsbBroadEn_02765
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC130466.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505997
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGCGACATAGTGGAGAAGACGCTGACGGCGCTGCCGGGACTCTTTCTGCAGAACCAG
CCCGGTGGTGGGCCCGCGGCCGCCAAGGCGTCCTTCTCCTCGAGGCTGGGCAGCCTTGTC
CGCGGCATCACAGCCCTCACCTCCAAGCACGAAGAAGAAAAATTAATCCAGCAGGAACTG
AGTAGTCTGAAAGCGACTGTTTCTGCTCCTACTACAACACTGAAAATGATGAAGGAATGT
ATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATGCTTCCTTTGGCTATATTCAT
GCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTAGGTTATTTGGCTGTT
TCCTTATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTGAATACAGTTGTAAAG
GATCTGCAGAGCACTAACCTAGTAGAAGTGTGTATGGCACTGACTGTTGTTAGCCAGATT
TTCCCCTGCGAAATGATTCCAGCTGTTCTTCCATTAATAGAAGATAAACTTCAACATTCT
AAGGAGATTGTACGAAGAAAAGCTGTTCTGGCATTATACAAATTCCATCTCATTGCTCCT
AATCAAGTACAACATATTCATATTAAGTTTCGGAAAGCACTTTGTGACAGAGATGTTGGG
GTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTAAGGAGAATTCATCTGGATAT
AAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTTGGAGGAAAGCTCCCA
GTAGAATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATTCAGCTCTTGAGAATA
CTGGGACTTCTAGGAAAAGATGATCAAAGGACAAGTGAATTAATGTATGATGTTCTTGAT
GAATCCTTACGAAGAGCTGAGTTAAATCACAATGTCACATATGCTATTTTGTTTGAATGT
GTGCATACAGTCTATTCTATTTATCCTAAATCGGAATTACTTGAGAAGGCTGCCAAGTGC
ATTGGAAAATTTGTTCTGTCACCTAAAATAAATCTAAAATATTTAGGACTGAAGGCTCTT
ACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAACACCAGATGACAATAATTGAA
TGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTACAGAATT
ACTAATGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAATATTTACATCAGAGC
AAAGAAGAGTATGTCATCGTCAATTTGGTCGGCAAAATAGCAGAGCTGGCTGAGAAATAT
GCTCCTGATAATGCATGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTAGGAGGAGAT
GTAATGCATCCTGATATTCCCAATAACTTTCTGAGACTACTAGCGGAAGGTTTTGATGAT
GAAACAGAAGATCAGCAATTAAGACTCTATGCAGTTCAGTCTTATCTCACTTTACTGGAT
ATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTATGAGTTGGGTATTAGGGGAA
TATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCTAAGCTCTACAAGTTA
CTTATGAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATTGCTGCTGTGACCAAA
TTGACATCTCAGGCGCACTCTTCTAATACAGTTGAGAGATTAATCCATGAATTTACCATA
TCTTTGGATACTTGTATGAGACAACATGCATTTGAATTAAAACATTTGCATGAGAATGTG
GAACTTATGAAGAGCTTGCTTCCAGTTGACAGGAGTTGTGAAGACTTGGTGGTAGATGCT
TCTTTATCTTTTCTGGATGGTTTTGTGGCTGAAGGACTCAGTCAGGGTGCAGCGCCTTAC
AAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGGAAAAAGTTCTCAATTTTGAA
CCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCTGCTGGCATT
TCTCTTGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTGAAAGAGACAAATAGC
TTGAAGCTGGAAGGTATAAAGAAATTGTGGGGGAAAGAAGGCTATCTTCCCAAGAAGGAA
AGCAAAACTGGTGATGAAAGTGGAGCTCTGCCTGTTCCTCAAGAGAGTATAATGGAGAAT
GTAGATCAAGCTATAACTAAAAAGGATCAATCTCAAGTTCTTACCCAATCTAAAGAGGAG
AAAGAAAAGCAGCTGCTGGCATCATCATTATTTGTTGGTCTAGGATCAGAAAGTACAATC
AACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCAGAAGGAAATCAAAAGTCAAA
GAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGTTCTTCCTTTAGTTCT
TTGTCAAATGTGGCATATGAAGATGATTATTATTCGAATACTTTGCACGATACAGGAGAC
AAGGAATTAAAGAAATTTTCTCTCACTTCAGAACTTTTGGATTCTGAGTCACTCACAGAA
CTGCCCTTGGTTGAGAAATTCTCATATTGTAGTCTGTCTACACCTTCATTGTTTGCTAAT
AACAACATGGAAATTTTTCACCCTCCTCAATCTACTGCAGCCTCAGTTGCCAAGGAAAGC
TCTTTAGCTTCATCTTTTTTGGAAGAAACTACTGAATACATACACTCAAATGCTATGGAA
GTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAATTTGGAAAGATGATTGTTTA
TTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAAAGTGCTGACTTAGAA
ATTTTTCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGCTGTTTGCCTGTAATG
GAAGCAGAAAGCACCAAAAGCTTTCAATATAGTGTGCAGATAGAAAAACCTTTTACAGAA
GGAAATCTTACTGGTTTTATTAGTTATCATATGATGGATACTCATTCTGCTCAGCTGGAA
TTTTCTGTAAACTTATCACTATTAGATTTCATTAGACCATTAAAAATCTCAAGTGACGAC
TTTGGGAAACTCTGGTTATCCTTCGCAAATGATGTGAAACAAAATGTAAAAATGTCAGAA
TCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAACAGAAACTAAGACTCCATATT
ATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTCCCATCCATCCCCTGC
TTACTGCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTCAGATCCTCCTGTTCT
ACTCTTCCTGACTATTTACTGTATCAGTGTCAAAAGGTGATGGAGGGATCC
Protein sequence predicted based on nucleotide sequence
MSDIVEKTLTALPGLFLQNQPGGGPAAAKASFSSRLGSLVRGITALTSKHEEEKLIQQEL SSLKATVSAPTTTLKMMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAV SLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQIFPCEMIPAVLPLIEDKLQHS KEIVRRKAVLALYKFHLIAPNQVQHIHIKFRKALCDRDVGVMAASLHIYLRMIKENSSGY KDLTGSFVTILKQVVGGKLPVEFNYHSVPAPWLQIQLLRILGLLGKDDQRTSELMYDVLD ESLRRAELNHNVTYAILFECVHTVYSIYPKSELLEKAAKCIGKFVLSPKINLKYLGLKAL TYVIQQDPTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNITVIVQKMLEYLHQS KEEYVIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIPNNFLRLLAEGFDD ETEDQQLRLYAVQSYLTLLDMENVFYPQRFLQVMSWVLGEYSYLLDKETPEEVIAKLYKL LMNDSVSSETKAWLIAAVTKLTSQAHSSNTVERLIHEFTISLDTCMRQHAFELKHLHENV ELMKSLLPVDRSCEDLVVDASLSFLDGFVAEGLSQGAAPYKPPHQRQEEKLSQEKVLNFE PYGLSFSSSGFTGRQSPAGISLGSDVSGNSAETGLKETNSLKLEGIKKLWGKEGYLPKKE SKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSLFVGLGSESTI NLLGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGD KELKKFSLTSELLDSESLTELPLVEKFSYCSLSTPSLFANNNMEIFHPPQSTAASVAKES SLASSFLEETTEYIHSNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLE IFPAENFKVTEQPGCCLPVMEAESTKSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLE FSVNLSLLDFIRPLKISSDDFGKLWLSFANDVKQNVKMSESQAALPSALKTLQQKLRLHI IEIIGNEGLLACQLLPSIPCLLHCRVHADVLALWFRSSCSTLPDYLLYQCQKVMEGS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
