Gene:
Gene Symbol:
STXBP5
Gene Name:
syntaxin binding protein 5 (tomosyn)
Original Clone ID:
ccsbBroadEn_09557
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC113382.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506027
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGGAAATTCAACATCAGGAAGGTGCTGGACGGCCTGACCGCCGGCTCGTCCTCGGCG
TCGCAGCAGCAACAGCAGCAGCATCCGCCTGGGAACCGGGAGCCGGAGATCCAGGAAACG
CTCCAGTCCGAGCACTTTCAGCTCTGCAAGACTGTTCGCCATGGATTTCCCTATCAACCC
TCAGCCCTGGCCTTTGATCCTGTACAGAAGATCCTGGCAGTGGGAACTCAGACTGGTGCT
TTAAGGCTCTTTGGTCGTCCAGGAGTAGAATGTTATTGCCAGCATGACAGTGGAGCTGCA
GTAATCCAGCTCCAGTTCCTGATTAATGAGGGAGCGCTTGTGAGTGCCTTGGCTGATGAC
ACCTTACACTTATGGAATTTACGTCAGAAGAGGCCTGCCATACTACATTCGCTTAAATTT
TGCAGAGAAAGGGTTACATTTTGCCATCTGCCTTTCCAGAGTAAGTGGCTCTATGTGGGC
ACTGAACGAGGTAATATACATATTGTCAATGTGGAGTCCTTCACACTCTCAGGCTACGTC
ATTATGTGGAATAAAGCCATTGAACTGTCATCTAAATCTCACCCAGGACCTGTGGTCCAT
ATAAGTGATAATCCAATGGACGAGGGAAAGCTTTTGATTGGCTTTGAATCTGGAACAGTA
GTTTTATGGGACCTCAAATCAAAGAAAGCCGACTACAGATACACATATGATGAGGCTATC
CACTCTGTTGCTTGGCATCATGAAGGAAAACAATTTATTTGCAGTCATTCAGATGGCACC
TTGACTATATGGAATGTAAGGTCCCCTGCTAAACCAGTACAGACAATCACTCCACATGGA
AAACAGTTAAAGGATGGGAAGAAGCCAGAACCATGCAAACCTATCCTCAAGGTGGAATTC
AAAACGACTAGATCTGGGGAGCCTTTTATTATTTTATCAGGAGGTTTGTCATATGATACT
GTAGGAAGAAGACCTTGCTTAACAGTGATGCATGGGAAAAGCACTGCTGTGCTAGAAATG
GACTATTCAATTGTTGATTTTCTAACGCTGTGTGAAACACCATACCCAAATGATTTTCAA
GAACCATATGCTGTGGTTGTTCTTCTAGAAAAGGATTTAGTACTTATAGACCTTGCACAA
AATGGATATCCTATATTTGAAAATCCCTACCCTTTGAGTATACATGAGTCCCCTGTTACA
TGTTGCGAATATTTTGCGGATTGTCCTGTGGACCTTATTCCTGCACTTTATTCTGTTGGA
GCTAGACAGAAACGTCAAGGTTACAGCAAAAAGGAATGGCCCATCAACGGAGGTAATTGG
GGCTTGGGTGCTCAAAGTTACCCAGAAATAATTATTACAGGGCATGCTGATGGGTCAGTT
AAGTTCTGGGATGCTTCTGCAATAACTCTACAAGTATTATATAAGCTAAAGACATCTAAA
GTATTTGAAAAGTCAAGAAATAAAGATGACAGGCCAAACACAGACATTGTAGATGAAGAT
CCATATGCCATTCAGATCATCTCCTGGTGTCCAGAAAGTAGAATGCTGTGCATCGCTGGA
GTTTCAGCTCATGTCATTATTTATAGATTCAGCAAGCAGGAAGTAATCACAGAAGTCATT
CCGATGCTTGAAGTTCGATTATTATATGAGATAAATGATGTGGAAACTCCGGAGGGTGAG
CAGCCACCACCTTTGCCAACACCCGTGGGAGGGTCCAACCCTCAGCCCATCCCTCCTCAG
TCTCATCCATCTACCAGTAGCAGTTCATCTGATGGGCTTCGTGATAATGTACCTTGTTTA
AAAGTTAAAAACTCACCACTTAAACAGTCTCCAGGTTATCAAACAGAACTAGTTATTCAG
TTGGTTTGGGTGGGTGGAGAACCACCACAACAAATAACCAGCCTGGCAGTCAATTCTTCC
TATGGACTGGTGGTTTTTGGCAATTGCAATGGCATTGCTATGGTTGACTACCTCCAGAAA
GCAGTGCTGCTCAACCTGGGCACTATTGAATTATATGGCTCTAATGATCCTTATCGGAGA
GAACCCCGATCTCCTCGTAAATCTCGACAGCCTTCAGGAGCCGGTCTGTGTGATATTAGT
GAAGGGACTGTTGTTCCAGAGGATCGCTGCAAATCTCCAACCTCTGCAAAGATGTCAAGG
AAGTTAAGCTTACCTACTGACCTAAAGCCTGATTTAGATGTAAAGGATAACTCCTTTAGC
CGATCACGGAGTTCAAGTGTAACAAGCATTGACAAAGAATCCCGAGAAGCGATCTCCGCT
CTTCATTTCTGTGAAACGTTTACTCGAAAGACGGACTCGTCCCCTTCCCCTTGTCTATGG
GTTGGAACAACGCTAGGAACAGTGCTTGTCATTGCACTGAACCTTCCCCCAGGGGGAGAG
CAAAGACTTCTTCAGCCAGTAATTGTGTCTCCAAGTGGTACTATATTGAGGTTAAAAGGT
GCAATCTTGAGAATGGCATTTCTGGATACCACAGGCTGCTTAATACCACCTGCGTATGAA
CCCTGGAGAGAGCACAATGTTCCTGAAGAAAAAGACGAAAAGGAGAAATTGAAAAAACGG
CGGCCTGTCTCAGTATCCCCCTCCTCTTCTCAGGAAATTAGTGAAAACCAGTATGCAGTG
ATATGTTCTGAAAAGCAAGCAAAAGTAATCTCACTGCCAACCCAGAACTGTGCTTATAAG
CAAAATATTACAGAGACCTCGTTTGTGCTTCGTGGAGATATTGTAGCATTGAGTAACAGT
ATCTGCCTTGCCTGTTTCTGTGCCAATGGACATATAATGACTTTTAGTTTGCCAAGTTTA
AGACCTCTGTTGGATGTGTATTACTTGCCCCTTACCAATATGCGGATAGCCAGAACGTTC
TGCTTTACCAACAATGGACAAGCATTATACCTTGTTTCACCTACAGAAATCCAGAGACTT
ACTTATAGTCAAGAGACCTGTGAAAATCTTCAGGAAATGTTGGGTGAACTCTTCACTCCT
GTAGAAACACCTGAAGCACCAAACAGGGGATTCTTTAAAGGCTTATTTGGAGGTGGTGCA
CAATCTCTTGACAGAGAAGAACTATTTGGAGAATCGTCCTCAGGAAAGGCTTCAAGGAGC
CTTGCACAGCATATTCCTGGCCCTGGTGGCATTGAAGGCGTAAAAGGGGCAGCATCTGGA
GTTGTTGGTGAATTAGCACGAGCCAGGCTGGCACTAGATGAAAGAGGGCAGAAACTTGGC
GATCTGGAAGAAAGAACTGCGGCCATGTTATCAAGTGCAGAGTCGTTTTCTAAACATGCT
CATGAGATTATGTTGAAATACAAAGATAAGAAGTGGTACCAGTTC
Protein sequence predicted based on nucleotide sequence
MRKFNIRKVLDGLTAGSSSASQQQQQQHPPGNREPEIQETLQSEHFQLCKTVRHGFPYQP SALAFDPVQKILAVGTQTGALRLFGRPGVECYCQHDSGAAVIQLQFLINEGALVSALADD TLHLWNLRQKRPAILHSLKFCRERVTFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYV IMWNKAIELSSKSHPGPVVHISDNPMDEGKLLIGFESGTVVLWDLKSKKADYRYTYDEAI HSVAWHHEGKQFICSHSDGTLTIWNVRSPAKPVQTITPHGKQLKDGKKPEPCKPILKVEF KTTRSGEPFIILSGGLSYDTVGRRPCLTVMHGKSTAVLEMDYSIVDFLTLCETPYPNDFQ EPYAVVVLLEKDLVLIDLAQNGYPIFENPYPLSIHESPVTCCEYFADCPVDLIPALYSVG ARQKRQGYSKKEWPINGGNWGLGAQSYPEIIITGHADGSVKFWDASAITLQVLYKLKTSK VFEKSRNKDDRPNTDIVDEDPYAIQIISWCPESRMLCIAGVSAHVIIYRFSKQEVITEVI PMLEVRLLYEINDVETPEGEQPPPLPTPVGGSNPQPIPPQSHPSTSSSSSDGLRDNVPCL KVKNSPLKQSPGYQTELVIQLVWVGGEPPQQITSLAVNSSYGLVVFGNCNGIAMVDYLQK AVLLNLGTIELYGSNDPYRREPRSPRKSRQPSGAGLCDISEGTVVPEDRCKSPTSAKMSR KLSLPTDLKPDLDVKDNSFSRSRSSSVTSIDKESREAISALHFCETFTRKTDSSPSPCLW VGTTLGTVLVIALNLPPGGEQRLLQPVIVSPSGTILRLKGAILRMAFLDTTGCLIPPAYE PWREHNVPEEKDEKEKLKKRRPVSVSPSSSQEISENQYAVICSEKQAKVISLPTQNCAYK QNITETSFVLRGDIVALSNSICLACFCANGHIMTFSLPSLRPLLDVYYLPLTNMRIARTF CFTNNGQALYLVSPTEIQRLTYSQETCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGA QSLDREELFGESSSGKASRSLAQHIPGPGGIEGVKGAASGVVGELARARLALDERGQKLG DLEERTAAMLSSAESFSKHAHEIMLKYKDKKWYQF
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
