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FGF12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF12
Gene Name:  fibroblast growth factor 12
Sequence             
Original Clone ID: ccsbBroadEn_06210
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC022524.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00506663

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 544nts         Open reading frame : 1 to 544
Reference Sequence Alignment

ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGA
TACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGAC
TACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAGTGAAG
GCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTTTTCACT
CCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACA
CTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAA
ATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCT
ATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAAGGGCGT
TCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAAGATTCA
ACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF12
Symbol Nomenclature : FGF12
SYNONYM : FGF12B; FHF1
Designation : FGF-12|FHF-1|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
Full Nomenclature : fibroblast growth factor 12
GENEID : 2257
GenBank Accession : DQ893775
HGNC : 3668
MIM : 601513
Vega : OTTHUMG00000156132
Target GenBank: DQ893775

 

Reference Sequence Alignment

  

HsCD00506663        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      --------------ATGAAGATTCTTGAGCTTTCTCTGAAGAAAGGAGTGCATATGCTGC
NM_021032.4         ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAAC
                                                                                

HsCD00506663        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      AGTGTCTCTGTGGAAGGAGCCTG-------------------------------------
NM_021032.4         AGCGACCGAGTGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTG
                                                                                

HsCD00506663        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      ---------------------------------------------------------AGG
NM_021032.4         TGCGAGAGGCACGTCCTCGGGGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGG
                                                                                

HsCD00506663        ------ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_004113.5         ------ATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
XM_005247227.1      TCCAGCGACAGCCCAAGTGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_021032.4         CCGGTGAGGCGGAGACCAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
                          .   . . .. :***********.******************************

HsCD00506663        CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_004113.5         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
XM_005247227.1      CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_021032.4         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
                    ************************************************************

HsCD00506663        AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_004113.5         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
XM_005247227.1      AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_021032.4         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
                    ************************************************************

HsCD00506663        GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_004113.5         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
XM_005247227.1      GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_021032.4         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
                    ************************************************************

HsCD00506663        TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_004113.5         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
XM_005247227.1      TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_021032.4         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
                    ************************************************************

HsCD00506663        TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_004113.5         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
XM_005247227.1      TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_021032.4         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
                    ************************************************************

HsCD00506663        GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_004113.5         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
XM_005247227.1      GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_021032.4         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
                    ************************************************************

HsCD00506663        AAACCTATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAA
NM_004113.5         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
XM_005247227.1      AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
NM_021032.4         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
                    *******************************.****************************

HsCD00506663        GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_004113.5         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
XM_005247227.1      GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_021032.4         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
                    ************************************************************

HsCD00506663        GATTCAACA---
NM_004113.5         GATTCAACATAG
XM_005247227.1      GATTCAACATAG
NM_021032.4         GATTCAACATAG
                    *********   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 615450
Original Clone ID : ccsbBroadEn_06210

Organism


TAX_ID : 9606
Species Specific ID: 2257

Genome

Chromosome : 3
Map Location : 3q28
Ensembl : ENSG00000114279

Reagents

Labome : FGF12-antibody