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CLEC4D (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CLEC4D
Gene Name:  C-type lectin domain family 4, member D
Sequence             
Original Clone ID: ccsbBroadEn_05435
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC032313.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 25071783
Title: Computational and experimental prediction of human C-type lectin receptor druggability.
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00507041

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 646nts         Open reading frame : 1 to 646
Reference Sequence Alignment

ATGGGGCTAGAAAAACCTCAAAGTAAACTGGAAGGAGGCATGCATCCCCAGCTGATACCT
TCGGTTATTGCTGTAGTTTTCATCTTACTTCTCAGTGTCTGTTTTATTGCAAGTTGTTTG
GTGACTCATCACAACTTTTCACGCTGTAAGAGAGGCACAGGAGTGCACAAGTTAGAGCAC
CATGCAAAGCTCAAATGCATCAAAGAGAAATCAGAACTGAAAAGTGCTGAAGGGAGCACC
TGGAACTGTTGTCCTATTGACTGGAGAGCCTTCCAGTCCAACTGCTATTTTCCTCTTACT
GACAACAAGACGTGGGCTGAGAGTGAAAGGAACTGTTCAGGGATGGGGGCCCATCTGATG
ACCATCAGCACGGAAGCTGAGCAGAACTTTATTATTCAGTTTCTGGATAGACGGCTTTCC
TATTTCCTTGGACTTAGAGATGAGAATGCCAAAGGTCAGTGGCGTTGGGTGGACCAGACG
CCATTTAACCCACGCAGAGTATTCTGGCATAAGAATGAACCCGACAACTCTCAGGGAGAA
AACTGTGTTGTTCTTGTTTATAACCAAGATAAATGGGCCTGGAATGATGTTCCTTGTAAC
TTTGAAGCAAGTAGGATTTGTAAAATACCTGGAACAACATTGAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CLEC4D
Symbol Nomenclature : CLEC4D
SYNONYM : CLEC-6; CLEC6; CLECSF8; MCL; MPCL
Designation : C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8|C-type lectin domain family 4 member D|C-type lectin receptor|C-type lectin superfamily member 8|C-type lectin-like receptor 6|macrophage C-type lectin
Full Nomenclature : C-type lectin domain family 4, member D
GENEID : 338339
GenBank Accession : DQ894429
HGNC : 14554
MIM : 609964
Vega : OTTHUMG00000168674
Target GenBank: DQ894429

 

Reference Sequence Alignment

  

HsCD00507041      ATGGGGCTAGAAAAACCTCAAAGTAAACTGGAAGGAGGCATGCATCCCCAGCTGATACCT
NM_080387.4       ATGGGGCTAGAAAAACCTCAAAGTAAACTGGAAGGAGGCATGCATCCCCAGCTGATACCT
                  ************************************************************

HsCD00507041      TCGGTTATTGCTGTAGTTTTCATCTTACTTCTCAGTGTCTGTTTTATTGCAAGTTGTTTG
NM_080387.4       TCGGTTATTGCTGTAGTTTTCATCTTACTTCTCAGTGTCTGTTTTATTGCAAGTTGTTTG
                  ************************************************************

HsCD00507041      GTGACTCATCACAACTTTTCACGCTGTAAGAGAGGCACAGGAGTGCACAAGTTAGAGCAC
NM_080387.4       GTGACTCATCACAACTTTTCACGCTGTAAGAGAGGCACAGGAGTGCACAAGTTAGAGCAC
                  ************************************************************

HsCD00507041      CATGCAAAGCTCAAATGCATCAAAGAGAAATCAGAACTGAAAAGTGCTGAAGGGAGCACC
NM_080387.4       CATGCAAAGCTCAAATGCATCAAAGAGAAATCAGAACTGAAAAGTGCTGAAGGGAGCACC
                  ************************************************************

HsCD00507041      TGGAACTGTTGTCCTATTGACTGGAGAGCCTTCCAGTCCAACTGCTATTTTCCTCTTACT
NM_080387.4       TGGAACTGTTGTCCTATTGACTGGAGAGCCTTCCAGTCCAACTGCTATTTTCCTCTTACT
                  ************************************************************

HsCD00507041      GACAACAAGACGTGGGCTGAGAGTGAAAGGAACTGTTCAGGGATGGGGGCCCATCTGATG
NM_080387.4       GACAACAAGACGTGGGCTGAGAGTGAAAGGAACTGTTCAGGGATGGGGGCCCATCTGATG
                  ************************************************************

HsCD00507041      ACCATCAGCACGGAAGCTGAGCAGAACTTTATTATTCAGTTTCTGGATAGACGGCTTTCC
NM_080387.4       ACCATCAGCACGGAAGCTGAGCAGAACTTTATTATTCAGTTTCTGGATAGACGGCTTTCC
                  ************************************************************

HsCD00507041      TATTTCCTTGGACTTAGAGATGAGAATGCCAAAGGTCAGTGGCGTTGGGTGGACCAGACG
NM_080387.4       TATTTCCTTGGACTTAGAGATGAGAATGCCAAAGGTCAGTGGCGTTGGGTGGACCAGACG
                  ************************************************************

HsCD00507041      CCATTTAACCCACGCAGAGTATTCTGGCATAAGAATGAACCCGACAACTCTCAGGGAGAA
NM_080387.4       CCATTTAACCCACGCAGAGTATTCTGGCATAAGAATGAACCCGACAACTCTCAGGGAGAA
                  ************************************************************

HsCD00507041      AACTGTGTTGTTCTTGTTTATAACCAAGATAAATGGGCCTGGAATGATGTTCCTTGTAAC
NM_080387.4       AACTGTGTTGTTCTTGTTTATAACCAAGATAAATGGGCCTGGAATGATGTTCCTTGTAAC
                  ************************************************************

HsCD00507041      TTTGAAGCAAGTAGGATTTGTAAAATACCTGGAACAACATTGAAC---
NM_080387.4       TTTGAAGCAAGTAGGATTTGTAAAATACCTGGAACAACATTGAACTAG
                  *********************************************   

Clone

Cloning Information : 615734
Original Clone ID : ccsbBroadEn_05435

Organism


TAX_ID : 9606
Species Specific ID: 338339

Genome

Chromosome : 12
Map Location : 12p13.31
Ensembl : ENSG00000166527

Reagents

Labome : CLEC4D-antibody