Gene:
Gene Symbol:
IFT122
Gene Name:
intraflagellar transport 122 homolog (Chlamydomonas)
Original Clone ID:
ccsbBroadEn_08583
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC003045.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00508149
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAATGACATCGCATTT
AAGCCTGATGGAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTGGTTTATGACACC
TCTGATGGCACCTTACTTCAGCCCCTCAAGGGACACAAAGACACTGTGTACTGTGTGGCA
TATGCGAAGGATGGGTTGTGGTCTCCTGAACAGAAGTCTGTCTCCAAACACAAATCAAGC
AGCAAGATCATCTGCTGCAGCTGGACAAATGATGGTCAGTACCTGGCGCTGGGGATGTTC
AATGGGATCATCAGCATACGGAACAAAAATGGCGAGGAGAAAGTAAAGATCGAGCGGCCG
GGGGGCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAACGTAAT
GACATCCTGGCTGTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGTGGAAAA
CAGATTGGAAAGGATCGGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACTAAA
GGCGAGTACATTTTGCTGGGGGGTTCAGACAAGCAAGTATCTCTTTTCACCAAGGATGGA
GTGCGGCTTGGGACTGTTGGGGAGCAGAACTCCTGGGTGTGGACGTGTCAAGCGAAACCG
GATTCCAACTATGTGGTGGTCGGCTGCCAGGACGGCACCATTTCCTTCTACCAGCTTATT
TTCAGCACAGTCCATGGGCTTTACAAGGACCGCTATGCCTACAGGGATAGCATGACTGAC
GTCATTGTGCAGCACCTGATCACTGAGCAGAAAGTTCGGATTAAATGCAAAGAGCTTGTC
AAGAAGATTGCCATCTACAGAAATCGATTGGCTATCCAACTGCCAGAGAAAATCCTCATC
TATGAGTTGTATTCAGAGGACTTATCAGACATGCATTACCGGGTAAAGGAGAAGATTATC
AAGAAGTTTGAGTGCAACCTCCTGGTGGTGTGTGCCAATCACATCATCCTGTGCCAGGAG
AAACGGCTGCAGTGCCTGTCCTTCAGCGGAGTGAAGGAGCGGGAGTGGCAGATGGAGTCT
CTCATTCGTTACATCAAGGTGATCGGTGGCCCTCCTGGAAGAGAAGGCCTCTTAGTGGGG
CTGAAGAATGGACAGATCCTGAAGATCTTCGTGGACAATCTCTTTGCTATCGTCCTGCTG
AAGCAGGCCACAGCTGTGCGCTGCTTGGACATGAGTGCCTCCCGTAAGAAGCTGGCCGTG
GTAGATGAAAATGACACTTGCCTGGTGTATGACATCGACACCAAGGAGCTGCTTTTTCAG
GAACCAAACGCCAACAGTGTAGCTTGGAACACCCAGTGTGAGGACATGCTCTGCTTCTCG
GGAGGAGGCTACCTCAACATCAAAGCCAGCACCTTCCCTGTGCACCGGCAGAAGCTGCAG
GGCTTTGTGGTCGGCTACAATGGCTCCAAGATCTTCTGCCTCCATGTCTTCTCCATTTCT
GCCGTGGAGGTGCCGCAGTCCGCTCCCATGTACCAGTACCTGGATAGGAAACTGTTCAAG
GAAGCCTACCAGATTGCTTGCTTGGGTGTCACAGACACTGATTGGCGTGAACTGGCCATG
GAAGCGCTAGAAGGTTTAGATTTTGAAACTGCAAAGAAGGCCTTCATCAGAGTACAAGAC
CTCCGATATTTAGAGCTCATCAGCAGCATTGAGGAGAGGAAGAAGCGGGGAGAGACCAAC
AATGACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTCCATGAGGCCGCCAAA
CTGTACAAGAGGAGTGGGCACGAGAACCTCGCGCTTGAAATGTACACCGACCTCTGCATG
TTTGAGTATGCCAAGGATTTCCTTGGATCTGGAGACCCCAAAGAAACAAAGATGCTAATC
ACCAAACAGGCTGACTGGGCCAGAAATATCAAGGAGCCCAAAGCCGCCGTGGAGATGTAC
ATCTCAGCAGGAGAGCACGTCAAGGCCATCGAGATCTGTGGTGACCATGGCTGGGTTGAC
ATGTTGATCGACATCGCCCGCAAACTGGACAAGGCTGAGCGCGAGCCCCTGCTGCTGTGC
GCTACCTACCTCAAGAAGCTGGACAGCCCTGGCTATGCTGCTGAGACCTACCTGAAGATG
GGTGACCTCAAGTCCCTGGTGCAGCTGCACGTGGAGACCCAGCGCTGGGATGAGGCCTTT
GCTTTGGGTGAGAAGCATCCTGAGTTTAAGGATGACATCTACATGCCGTATGCTCAGTGG
CTAGCAGAGAACGATCGCTTTGAGGAAGCCCAGAAAGCGTTCCACAAGGCTGGGCGACAG
AGAGAAGCGGTCCAGGTGCTGGAGCAGCTCACAAACAATGCCGTGGCGGAGAGCAGGTTT
AATGATGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCTCGATATAGCTCAAGCA
GATCCTGCCCAGAAGGACACAATGCTTGGCAAGTTCTACCACTTCCAGCGTTTGGCAGAG
CTGTACCATGGTTACCATGCCATCCATCGCCACACGGAAGATCCGTTCAGTGTCCATCGT
CCTGAAACTCTTTTCAACATCTCCAGGTTCCTGCTGCACAGCCTGCCCAAGGACACCCCC
TCGGGCATCTCTAAAGTGAAAATACTCTTCACCTTGGCCAAGCAGAGCAAGGCCCTCGGT
GCCTACAGGCTGGCCCGGCACGCCTATGACAAGCTGCGTGGCCTGTACATCCCTGCCAGA
TTCCAAAAGTCCATTGAGCTGGGTACCCTGACCATCCGCGCCAAGCCCTTCCACGACAGT
GAGGAGTTGGTGCCCTTGTGCTACCGCTGCTCCACCAACAACCCGCTGCTCAACAACCTG
GGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTACGACGTG
CTACACCTGGTTGAGTTCTACCTGGAGGAAGGGATCACTGATGAAGAAGCCATCTCCCTC
ATCGACCTGGAGGTGCTGAGACCCAAGCGGGATGACAGACAGCTAGAGATTGCAAACAAC
AGCTCCCAGATTCTGCGGCTAGTGGAGACCAAGGACTCCATCGGAGATGAGGACCCGTTC
ACAGCTAAGCTGAGCTTTGAGCAAGGTGGCTCAGAGTTCGTGCCAGTGGTGGTGAGCCGG
CTGGTGCTGCGCTCCATGAGCCGCCGGGATGTCCTCATCAAGCGATGGCCCCCACCCCTG
AGGTGGCAATACTTCCGCTCACTGCTGCCTGACGCCTCCATTACCATGTGCCCCTCCTGC
TTCCAGATGTTCCATTCTGAGGACTATGAGTTGCTGGTGCTTCAGCATGGCTGCTGCCCC
TACTGCCGCAGGTGCAAGGATGACCCTGGCCCA
Protein sequence predicted based on nucleotide sequence
MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVA YAKDGLWSPEQKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERP GGSLSPIWSICWNPSREERNDILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTK GEYILLGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCQAKPDSNYVVVGCQDGTISFYQLI FSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYRNRLAIQLPEKILI YELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSGVKEREWQMES LIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRKKLAV VDENDTCLVYDIDTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHRQKLQ GFVVGYNGSKIFCLHVFSISAVEVPQSAPMYQYLDRKLFKEAYQIACLGVTDTDWRELAM EALEGLDFETAKKAFIRVQDLRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAK LYKRSGHENLALEMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWARNIKEPKAAVEMY ISAGEHVKAIEICGDHGWVDMLIDIARKLDKAEREPLLLCATYLKKLDSPGYAAETYLKM GDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYMPYAQWLAENDRFEEAQKAFHKAGRQ REAVQVLEQLTNNAVAESRFNDAAYYYWMLSMQCLDIAQADPAQKDTMLGKFYHFQRLAE LYHGYHAIHRHTEDPFSVHRPETLFNISRFLLHSLPKDTPSGISKVKILFTLAKQSKALG AYRLARHAYDKLRGLYIPARFQKSIELGTLTIRAKPFHDSEELVPLCYRCSTNNPLLNNL GNVCINCRQPFIFSASSYDVLHLVEFYLEEGITDEEAISLIDLEVLRPKRDDRQLEIANN SSQILRLVETKDSIGDEDPFTAKLSFEQGGSEFVPVVVSRLVLRSMSRRDVLIKRWPPPL RWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHGCCPYCRRCKDDPGP
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
