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FGF14 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF14
Gene Name:  fibroblast growth factor 14
Sequence             
Original Clone ID: ccsbBroadEn_00560
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC100920.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00509587

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 757nts         Open reading frame : 1 to 757
Reference Sequence Alignment

ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF14
Symbol Nomenclature : FGF14
SYNONYM : FGF-14; FHF-4; FHF4; SCA27
Designation : bA397O8.2|fibroblast growth factor homologous factor 4
Full Nomenclature : fibroblast growth factor 14
GENEID : 2259
Locus Tag : RP11-397O8.6
HGNC : 3671
MIM : 601515
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00509587      ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
NM_175929.2       ATGGTAAAACCGGTGCCCCTCTTCAGGAGAACTGATTTCAAATTATTATTATGCAACCAC
NM_004115.3       ------------ATGGCCGCGGCCATCGCTAGCGGCTTGATCCGCCAGAAGCGGCAGGCG
                              .** **     **  . :*  *. ** *:.  . :.::. * .*  . 

HsCD00509587      AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
NM_175929.2       AAGGATCTCTTCTTTCTCAGGGTGTCTAAGCTGCTGGATTGCTTTTCGCCCAAATCAATG
NM_004115.3       CGGGAGCAGCACTGGGACCGGCCGTCTGCCAGCAGGAGGCGGAGCAGCCCCAGCAAGAAC
                  ..*** *:  :**   :*.**  ****.. .  . *..  * :  :  ****..:..*: 

HsCD00509587      TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
NM_175929.2       TGGTTTCTTTGGAACATTTTCAGCAAAGGAACGCATATGCTGCAGTGTCTTTGTGGCAAG
NM_004115.3       CGCGGGCTCTGCAACGGCAACCTGGTGGATATCTTCTCCAAAGTGCGCATCTTCGGCCTC
                   *    ** ** ***.  ::*.  .:.*.:*   : :  .:. :* * .* *  ***.: 

HsCD00509587      AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
NM_175929.2       AGTCTTAAGAAAAACAAGAACCCAACTGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
NM_004115.3       AAGAAGCG---CAGGTTGCGGCGCCAAGATCCCCAGCTCAAGGGTATAGTGACCAGGTTA
                  *. .: ..   .*. ::*.. * ...:*********************************

HsCD00509587      TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
NM_175929.2       TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
NM_004115.3       TATTGCAGGCAAGGCTACTACTTGCAAATGCACCCCGATGGAGCTCTCGATGGAACCAAG
                  ************************************************************

HsCD00509587      GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
NM_175929.2       GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
NM_004115.3       GATGACAGCACTAATTCTACACTCTTCAACCTCATACCAGTGGGACTACGTGTTGTTGCC
                  ************************************************************

HsCD00509587      ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
NM_175929.2       ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
NM_004115.3       ATCCAGGGAGTGAAAACAGGGTTGTATATAGCCATGAATGGAGAAGGTTACCTCTACCCA
                  ************************************************************

HsCD00509587      TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
NM_175929.2       TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
NM_004115.3       TCAGAACTTTTTACCCCTGAATGCAAGTTTAAAGAATCTGTTTTTGAAAATTATTATGTA
                  ************************************************************

HsCD00509587      ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
NM_175929.2       ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
NM_004115.3       ATCTACTCATCCATGTTGTACAGACAACAGGAATCTGGTAGAGCCTGGTTTTTGGGATTA
                  ************************************************************

HsCD00509587      AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
NM_175929.2       AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
NM_004115.3       AATAAGGAAGGGCAAGCTATGAAAGGGAACAGAGTAAAGAAAACCAAACCAGCAGCTCAT
                  ************************************************************

HsCD00509587      TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
NM_175929.2       TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
NM_004115.3       TTTCTACCCAAGCCATTGGAAGTTGCCATGTACCGAGAACCATCTTTGCATGATGTTGGG
                  ************************************************************

HsCD00509587      GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
NM_175929.2       GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
NM_004115.3       GAAACGGTCCCGAAGCCTGGGGTGACGCCAAGTAAAAGCACAAGTGCGTCTGCAATAATG
                  ************************************************************

HsCD00509587      AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACA---
NM_175929.2       AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACATAG
NM_004115.3       AATGGAGGCAAACCAGTCAACAAGAGTAAGACAACATAG
                  ************************************   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 666280
Original Clone ID : ccsbBroadEn_00560

Organism


TAX_ID : 9606
Species Specific ID: 2259

Genome

Chromosome : 13
Map Location : 13q34

Reagents

Labome : FGF14-antibody