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LGALS12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS12
Gene Name:  lectin, galactoside-binding, soluble, 12
Sequence             
Original Clone ID: ccsbBroadEn_04477
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC028222.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510060

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1009nts         Open reading frame : 1 to 1009
Reference Sequence Alignment

ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAGGTTTCAGGTGGACTTC
CAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGCTTC
CATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGGGAG
GCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTCTTC
GGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTACCGG
CTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCTGTT
GGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACATCCT
TTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGTCTC
TCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTTACT
GTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCAGAC
AGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTCCTC
TTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAGCTG
GCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAGCTG
CGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS12
Symbol Nomenclature : LGALS12
SYNONYM : GAL12; GRIP1
Designation : galectin 12|galectin-12|galectin-related inhibitor of proliferation
Full Nomenclature : lectin, galactoside-binding, soluble, 12
GENEID : 85329
GenBank Accession : JF432638
HGNC : 15788
MIM : 606096
Vega : OTTHUMG00000167807
Target GenBank: JF432638

 

Reference Sequence Alignment

  

HsCD00510060        ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_001142535.1      ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_033101.3         ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
NM_001142536.1      ATGAGTCAGCCCAGTGGGGGCAGGGCTCCTGGAACGAGGATCTACAGTTGGAGTTGCCCC
                    ************************************************************

HsCD00510060        ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_001142535.1      ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_033101.3         ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
NM_001142536.1      ACTGTCATGTCACCTGGAGAAAAACTGGACCCAATTCCTGACAGCTTCATTCTGCAACCA
                    ************************************************************

HsCD00510060        CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_001142535.1      CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_033101.3         CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
NM_001142536.1      CCAGTCTTCCACCCGGTGGTTCCTTATGTCACGACGATTTTTGGAGGCCTGCATGCAGGC
                    ************************************************************

HsCD00510060        AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
NM_001142535.1      AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAGTAGGTTTCAGGTGGAC
NM_033101.3         AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
NM_001142536.1      AAGATGGTCATGCTGCAAGGAGTGGTCCCTCTAGATGCACACAG---GTTTCAGGTGGAC
                    ********************************************   *************

HsCD00510060        TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_001142535.1      TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_033101.3         TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
NM_001142536.1      TTCCAGTGTGGCTGCAGCCTGTGTCCCCGGCCAGATATCGCCTTCCACTTCAACCCTCGC
                    ************************************************************

HsCD00510060        TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_001142535.1      TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_033101.3         TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
NM_001142536.1      TTCCATACCACCAAGCCCCATGTCATCTGCAACACCCTGCATGGTGGACGCTGGCAAAGG
                    ************************************************************

HsCD00510060        GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_001142535.1      GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_033101.3         GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
NM_001142536.1      GAGGCCCGGTGGCCCCACCTGGCCCTGCGAAGAGGCTCCAGCTTCCTCATCCTCTTTCTC
                    ************************************************************

HsCD00510060        TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_001142535.1      TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_033101.3         TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
NM_001142536.1      TTCGGGAATGAGGAAGTGAAGGTGAGTGTGAATGGACAGCACTTTCTCCACTTCCGCTAC
                    ************************************************************

HsCD00510060        CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_001142535.1      CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_033101.3         CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
NM_001142536.1      CGGCTCCCACTGTCTCATGTGGACACGCTGGGTATATTTGGTGACATCCTGGTAGAGGCT
                    ************************************************************

HsCD00510060        GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_001142535.1      GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_033101.3         GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTGGACAT
NM_001142536.1      GTTGGATTCCTGAACATCAATCCATTTGTGGAGGGCAGCAGAGAGTACCCAGCTG-----
                    *******************************************************     

HsCD00510060        CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_001142535.1      CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_033101.3         CCTTTCCTGCTGATGAGCCCCAGGCTGGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
NM_001142536.1      ----------------------GACATGAGGTGCCCTGCTCACATGCTCTTCCCCAGGGT
                                          *.*: *********************************

HsCD00510060        CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_001142535.1      CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_033101.3         CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
NM_001142536.1      CTCTCGCCTGGGCAGGTCATCATAGTACGGGGACTGGTCTTGCAAGAGCCGAAGCATTTT
                    ************************************************************

HsCD00510060        ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_001142535.1      ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_033101.3         ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
NM_001142536.1      ACTGTGAGCCTGAGGGACCAGGCTGCCCATGCTCCTGTGACACTCAGGGCCTCCTTCGCA
                    ************************************************************

HsCD00510060        GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_001142535.1      GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_033101.3         GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
NM_001142536.1      GACAGAACTCTGGCCTGGATCTCCCGCTGGGGGCAGAAGAAACTGATCTCAGCCCCCTTC
                    ************************************************************

HsCD00510060        CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_001142535.1      CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_033101.3         CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
NM_001142536.1      CTCTTTTACCCCCAGAGATTCTTTGAGGTGCTGCTCCTGTTCCAGGAGGGAGGGCTGAAG
                    ************************************************************

HsCD00510060        CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_001142535.1      CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_033101.3         CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
NM_001142536.1      CTGGCGCTCAATGGGCAGGGGCTGGGGGCCACCAGCATGAACCAGCAGGCCCTGGAGCAG
                    ************************************************************

HsCD00510060        CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCC---
NM_001142535.1      CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
NM_033101.3         CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
NM_001142536.1      CTGCGGGAGCTCCGGATCAGTGGAAGTGTCCAGCTCTACTGTGTCCACTCCTGA
                    ***************************************************   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
HPRD : 16196

Clone

Cloning Information : 668788
Original Clone ID : ccsbBroadEn_04477

Organism


TAX_ID : 9606
Species Specific ID: 85329

Genome

Chromosome : 11
Map Location : 11q13
Ensembl : ENSG00000133317

Reagents

Labome : LGALS12-antibody