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GPR17 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GPR17
Gene Name:  G protein-coupled receptor 17
Sequence             
Original Clone ID: ccsbBroadEn_06311
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC031653.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510113

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1018nts         Open reading frame : 1 to 1018
Reference Sequence Alignment

ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATCACCAACTTCTCCCTGGCCACGGCA
GAGCAATGTGGCCAGGAGACGCCACTGGAGAACATGCTGTTCGCCTCCTTCTACCTTCTA
GATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCTCTGTGGTTTTTCATCCGAGACCAC
AAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCATCTGGCCGTGGCCGACTTGTCGTGC
GTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTCTCTGGGAACCACTGGCCATTTGGG
GAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTACCTCAACATGTACGCCAGCATCTAC
TTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCCATTGTGCACCCGGTCAAGTCCCTC
AAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGTGCCTTCCTGTGGGTGGTGGTGGCT
GTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACCGTGCAGACCAACCACACGGTGGTC
TGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCATGCCCTGGTGTCCCTGGCAGTGGCC
TTCACCTTCCCGTTCATCACCACGGTCACCTGCTACCTGCTGATCATCCGCAGCCTGCGG
CAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAGGCAGTGCGCATGATCGCCATAGTG
CTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCACGTCAACCGCTCCGTCTACGTGCTG
CACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAGCGCATCCTGGCCCTGGCAAACCGC
ATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTCGACCCCATCATGTATTTCTTCGTG
GCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTCTGTGGCAAAAGGCTCAAGGGCCCG
CCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCGCTGAGTGCCAAGTCAGAGCTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GPR17
Symbol Nomenclature : GPR17
Designation : G-protein coupled receptor 17|P2Y-like receptor|R12|UDP/CysLT receptor|uracil nucleotide/cysteinyl leukotriene receptor
Full Nomenclature : G protein-coupled receptor 17
GENEID : 2840
GenBank Accession : DQ895665
HGNC : 4471
MIM : 603071
Vega : OTTHUMG00000131533
Target GenBank: DQ895665

 

Reference Sequence Alignment

  

HsCD00510113        ------------------------------------------------------------
NM_001161416.1      ------------------------------------------------------------
NM_001161415.1      ATGTCCAAACGGAGTTGGTGGGCTGGATCCAGAAAGCCCCCAAGAGAGATGCTGAAACTC
NM_001161417.1      ------------------------------------------------------------
NM_005291.2         ATGTCCAAACGGAGTTGGTGGGCTGGATCCAGAAAGCCCCCAAGAGAGATGCTGAAACTC
                                                                                

HsCD00510113        ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161416.1      ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161415.1      TCAGGCTCTGACTCCAGCCAAAGCATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_001161417.1      ------------------------ATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
NM_005291.2         TCAGGCTCTGACTCCAGCCAAAGCATGAATGGCCTTGAAGTGGCTCCCCCAGGTCTGATC
                                            ************************************

HsCD00510113        ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161416.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161415.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_001161417.1      ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
NM_005291.2         ACCAACTTCTCCCTGGCCACGGCAGAGCAATGTGGCCAGGAGACGCCACTGGAGAACATG
                    ************************************************************

HsCD00510113        CTGTTCGCCTCCTTCTACCTTCTAGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161416.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161415.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_001161417.1      CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
NM_005291.2         CTGTTCGCCTCCTTCTACCTTCTGGATTTTATCCTGGCTTTAGTTGGCAATACCCTGGCT
                    ***********************.************************************

HsCD00510113        CTGTGGTTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161416.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161415.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_001161417.1      CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
NM_005291.2         CTGTGGCTTTTCATCCGAGACCACAAGTCCGGGACCCCGGCCAACGTGTTCCTGATGCAT
                    ****** *****************************************************

HsCD00510113        CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161416.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161415.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_001161417.1      CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
NM_005291.2         CTGGCCGTGGCCGACTTGTCGTGCGTGCTGGTCCTGCCCACCCGCCTGGTCTACCACTTC
                    ************************************************************

HsCD00510113        TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161416.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161415.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_001161417.1      TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
NM_005291.2         TCTGGGAACCACTGGCCATTTGGGGAAATCGCATGCCGTCTCACCGGCTTCCTCTTCTAC
                    ************************************************************

HsCD00510113        CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161416.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161415.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_001161417.1      CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
NM_005291.2         CTCAACATGTACGCCAGCATCTACTTCCTCACCTGCATCAGCGCCGACCGTTTCCTGGCC
                    ************************************************************

HsCD00510113        ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161416.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161415.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_001161417.1      ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
NM_005291.2         ATTGTGCACCCGGTCAAGTCCCTCAAGCTCCGCAGGCCCCTCTACGCACACCTGGCCTGT
                    ************************************************************

HsCD00510113        GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161416.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161415.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_001161417.1      GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
NM_005291.2         GCCTTCCTGTGGGTGGTGGTGGCTGTGGCCATGGCCCCGCTGCTGGTGAGCCCACAGACC
                    ************************************************************

HsCD00510113        GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161416.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161415.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_001161417.1      GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
NM_005291.2         GTGCAGACCAACCACACGGTGGTCTGCCTGCAGCTGTACCGGGAGAAGGCCTCCCACCAT
                    ************************************************************

HsCD00510113        GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161416.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161415.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_001161417.1      GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
NM_005291.2         GCCCTGGTGTCCCTGGCAGTGGCCTTCACCTTCCCGTTCATCACCACGGTCACCTGCTAC
                    ************************************************************

HsCD00510113        CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161416.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161415.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_001161417.1      CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
NM_005291.2         CTGCTGATCATCCGCAGCCTGCGGCAGGGCCTGCGTGTGGAGAAGCGCCTCAAGACCAAG
                    ************************************************************

HsCD00510113        GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161416.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161415.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_001161417.1      GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
NM_005291.2         GCAGTGCGCATGATCGCCATAGTGCTGGCCATCTTCCTGGTCTGCTTCGTGCCCTACCAC
                    ************************************************************

HsCD00510113        GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161416.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161415.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_001161417.1      GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
NM_005291.2         GTCAACCGCTCCGTCTACGTGCTGCACTACCGCAGCCATGGGGCCTCCTGCGCCACCCAG
                    ************************************************************

HsCD00510113        CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161416.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161415.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_001161417.1      CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
NM_005291.2         CGCATCCTGGCCCTGGCAAACCGCATCACCTCCTGCCTCACCAGCCTCAACGGGGCACTC
                    ************************************************************

HsCD00510113        GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161416.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161415.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_001161417.1      GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
NM_005291.2         GACCCCATCATGTATTTCTTCGTGGCTGAGAAGTTCCGCCACGCCCTGTGCAACTTGCTC
                    ************************************************************

HsCD00510113        TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161416.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161415.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_001161417.1      TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
NM_005291.2         TGTGGCAAAAGGCTCAAGGGCCCGCCCCCCAGCTTCGAAGGGAAAACCAACGAGAGCTCG
                    ************************************************************

HsCD00510113        CTGAGTGCCAAGTCAGAGCTG---
NM_001161416.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_001161415.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_001161417.1      CTGAGTGCCAAGTCAGAGCTGTGA
NM_005291.2         CTGAGTGCCAAGTCAGAGCTGTGA
                    *********************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : Eicosanoid ligand-binding receptors
Reactome : G alpha (i) signalling events
Reactome : G alpha (q) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Gastrin-CREB signalling pathway via PKC and MAPK
Reactome : Leukotriene receptors
Reactome : Nucleotide-like (purinergic) receptors
Reactome : P2Y receptors
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like
WikiPathway : GPCRs, Other

Protein

HPRD : 04351

Clone

Cloning Information : 609108
Original Clone ID : ccsbBroadEn_06311

Organism


TAX_ID : 9606
Species Specific ID: 2840

Genome

Chromosome : 2
Map Location : 2q21
Ensembl : ENSG00000144230

Reagents

Labome : GPR17-antibody