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ACTR1B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR1B
Gene Name:  ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Sequence             
Original Clone ID: ccsbBroadEn_07554
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC006372.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00510886

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts         Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGCCTACTCCAAGGATCAGCTGCAG
ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR1B
Symbol Nomenclature : ACTR1B
SYNONYM : ARP1B; CTRN2; PC3
Designation : actin-related protein 1B|beta-centractin|centractin beta
Full Nomenclature : ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GENEID : 10120
GenBank Accession : DQ896748
HGNC : 168
MIM : 605144
Vega : OTTHUMG00000130549
Target GenBank: DQ896748

 

Reference Sequence Alignment

  

HsCD00510886      ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005735.3       ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005736.3       ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
                  ************** .* ***************** ** ***********:** ** ***

HsCD00510886      ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005735.3       ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005736.3       ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
                  *********** *****:**:** ***** *********** ** *********** ** 

HsCD00510886      CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005735.3       CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005736.3       CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
                  **.** ******.* ** ** *****:******** **.** ***.******* **.**.

HsCD00510886      AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005735.3       AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005736.3       AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
                  *****:***********.******** :*.******** *********** ***.* ** 

HsCD00510886      CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGCCTACTCCAAGGATCAGCTGCAG
NM_005735.3       CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005736.3       AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
                  ...** ******************** *****.** * *** ** ***** *********

HsCD00510886      ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005735.3       ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005736.3       ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
                  ** *****.******************** ** ***** **  *.*****..*:**.***

HsCD00510886      CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005735.3       CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005736.3       CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
                  *****....** **.**.** ***** *********** ***** ** ** *********

HsCD00510886      ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005735.3       ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005736.3       ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
                  *****.*****.***** ** *****:*****..* ** *****.***** **.** **:

HsCD00510886      GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005735.3       GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005736.3       GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
                  ***** **.***** *********************************** *********

HsCD00510886      ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005735.3       ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005736.3       ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
                  ***** .* ***** ** ***** ******** ****:*** **.*** : ***** ***

HsCD00510886      GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005735.3       GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005736.3       GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
                  **.**  : ******** :*.**  ***********.*****..*.*.**.*****..**

HsCD00510886      GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005735.3       GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005736.3       GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
                  ** ** *****.*****.*****.**.*********.* **.*****.*****.*  ***

HsCD00510886      TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005735.3       TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005736.3       TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
                  ***:.  ****:** ******** .**** .* ** *** *.************** ***

HsCD00510886      CTGCTGTTCCAGCCGGACCTTGTGGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005735.3       CTGCTGTTCCAGCCGGACCTTGTCGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005736.3       TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
                   **** ***..***.**  * .* **.** ********.** .**** *****  ***  

HsCD00510886      TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005735.3       TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005736.3       TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
                  ********:** *****.************** ** ***** *** * ***** ** ***

HsCD00510886      TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005735.3       TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005736.3       TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
                  *****:*****:** ** ******** ** **:***.*. *.** **************.

HsCD00510886      CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005735.3       CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005736.3       CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
                  **:** *****.***.* **.****.***.**:**.** *****..*.***** *****.

HsCD00510886      TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005735.3       TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005736.3       TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
                  ******** *********** ***** ******** ***************** ******

HsCD00510886      AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTC---
NM_005735.3       AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTAG
NM_005736.3       AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
                  *****.*****.**.** **  ****: * ** ** .*.***** ***   

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Adaptive Immune System
Reactome : Immune System
Reactome : MHC class II antigen presentation

Protein

SMART domain : ACTIN : Actin
HPRD : 05508

Clone

Cloning Information : 610688
Original Clone ID : ccsbBroadEn_07554

Organism


TAX_ID : 9606
Species Specific ID: 10120

Genome

Chromosome : 2
Map Location : 2q11.1-q11.2
Ensembl : ENSG00000115073

Reagents

Labome : ACTR1B-antibody