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ACTRT1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTRT1
Gene Name:  actin-related protein T1
Sequence             
Original Clone ID: ccsbBroadEn_04932
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC014597.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00510900

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts        
Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTRT1
Symbol Nomenclature : ACTRT1
SYNONYM : AIP1; ARIP1; ARPT1; HSD27
Full Nomenclature : actin-related protein T1
GENEID : 139741
Locus Tag : RP1-203C2.1
GenBank Accession : EU832268
HGNC : 24027
MIM : 300487
Vega : OTTHUMG00000022359
Target GenBank: EU832268

 

Reference Sequence Alignment

  

HsCD00510900      ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
NM_138289.3       ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTC
                  ************************************************************

HsCD00510900      TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
NM_138289.3       TGCAAAGCAGGCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGA
                  ************************************************************

HsCD00510900      CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
NM_138289.3       CATTGTAAATTCAATGTGCCTTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAA
                  ************************************************************

HsCD00510900      GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
NM_138289.3       GCCCTGTACAAGTATGAGGCCCTACATTTGCACTACCCCATTGAGCGTGGACTGGTAACA
                  ************************************************************

HsCD00510900      GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
NM_138289.3       GGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTGAGCGGGAGCTTGGAGTAAAA
                  ************************************************************

HsCD00510900      CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
NM_138289.3       CCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGAAATTCGAGAA
                  ************************************************************

HsCD00510900      AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
NM_138289.3       AAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
                  ************************************************************

HsCD00510900      GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
NM_138289.3       GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGAT
                  ************************************************************

HsCD00510900      GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
NM_138289.3       GGGGTCACTTGCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAA
                  ************************************************************

HsCD00510900      CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
NM_138289.3       CTCTGTATGGCAGGGAGGGACATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGG
                  ************************************************************

HsCD00510900      TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
NM_138289.3       TTTAACTTCCCTTGCATACTCAACAAGGCCGTGGTAAATAACATCAAAGAGAAGTTGTGC
                  ************************************************************

HsCD00510900      TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
NM_138289.3       TACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCCGGGGAGAGGTCCTGGGAGCA
                  ************************************************************

HsCD00510900      TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
NM_138289.3       TACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCAAGTGCCCGAG
                  ************************************************************

HsCD00510900      GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
NM_138289.3       GTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
                  ************************************************************

HsCD00510900      AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
NM_138289.3       AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTC
                  ************************************************************

HsCD00510900      TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
NM_138289.3       TCCGGGGGCACCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAG
                  ************************************************************

HsCD00510900      CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
NM_138289.3       CTGGCTTCCAAAGGTACTCCCATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCA
                  ************************************************************

HsCD00510900      TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
NM_138289.3       TGGATTGGTGCATCCATCATGACCTCTATGAGCAGTTTCAAGCAGATGTGGGTCACCTCG
                  ************************************************************

HsCD00510900      GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTT---
NM_138289.3       GCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAAGGTGCTTTTAA
                  ************************************************   

Protein

SMART domain : ACTIN : Actin
HPRD : 06439

Clone

Cloning Information : 668701
Original Clone ID : ccsbBroadEn_04932

Organism


TAX_ID : 9606
Species Specific ID: 139741

Genome

Chromosome : X
Map Location : Xq25
Ensembl : ENSG00000123165

Reagents

Labome : ACTRT1-antibody