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FAM46C (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FAM46C
Gene Name:  family with sequence similarity 46, member C
Sequence             
Original Clone ID: ccsbBroadEn_08421
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC036516.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00511099

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1174nts         Open reading frame : 1 to 1174
Reference Sequence Alignment

ATGGCAGAGGAGAGCAGCTGTACCAGGGATTGCATGTCCTTCAGCGTGCTCAACTGGGAT
CAGGTTAGCCGGCTGCATGAGGTCCTCACTGAAGTTGTACCTATCCACGGACGAGGCAAC
TTTCCAACCTTGGAGATAACTCTGAAGGACATCGTCCAGACCGTCCGCAGTCGGCTGGAG
GAGGCAGGCATCAAAGTGCATGACGTCCGGCTGAATGGCTCCGCAGCTGGCCACGTTTTG
GTCAAAGACAATGGCTTGGGCTGCAAAGACCTGGACCTAATCTTCCATGTGGCTCTTCCA
ACAGAGGCAGAATTTCAGCTGGTTAGAGATGTGGTTCTGTGTTCCCTTCTGAACTTCCTG
CCAGAGGGTGTGAACAAGCTCAAAATCAGTCCAGTCACTCTGAAGGAGGCATATGTGCAG
AAGCTAGTGAAGGTTTGCACGGACACTGACCGCTGGAGCCTGATCTCCCTCTCCAACAAG
AACGGGAAGAACGTGGAGCTGAAGTTTGTCGACTCCATTCGGCGTCAGTTTGAGTTCAGT
GTGGACTCTTTCCAAATCATCCTGGATTCTTTGCTTTTCTTCTATGACTGTTCCAATAAT
CCCATCTCTGAGCACTTCCACCCCACCGTGATTGGGGAGAGCATGTACGGGGACTTTGAG
GAAGCTTTTGACCATCTGCAGAACAGACTGATCGCCACCAAGAACCCAGAAGAAATCAGA
GGCGGGGGACTTCTCAAGTACAGCAACCTTCTTGTGCGGGACTTCAGGCCCACAGACCAG
GAAGAAATCAAAACTCTAGAGCGCTACATGTGCTCCAGGTTCTTCATCGACTTCCCGGAC
ATCCTTGAACAGCAGAGGAAGTTGGAGACTTACCTTCAAAACCACTTCGCTGAAGAAGAG
AGAAGCAAGTACGACTACCTCATGATCCTTCGCAGGGTGGTGAACGAGAGCACCGTGTGT
CTCATGGGGCATGAACGCAGGCAGACTCTGAACCTCATCTCCCTCCTGGCCTTGCGTGTG
CTGGCGGAACAAAACATCATCCCCAGTGCCACCAACGTCACCTGTTACTACCAGCCGGCC
CCTTACGTCAGTGATGGCAACTTCAGCAACTACTACGTTGCCCATCCTCCAGTCACCTAC
AGCCAGCCTTACCCTACCTGGCTGCCCTGTAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FAM46C
Symbol Nomenclature : FAM46C
Designation : protein FAM46C
Full Nomenclature : family with sequence similarity 46, member C
GENEID : 54855
HGNC : 24712
MIM : 613952
Vega : OTTHUMG00000013703
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00511099      ATGGCAGAGGAGAGCAGCTGTACCAGGGATTGCATGTCCTTCAGCGTGCTCAACTGGGAT
NM_017709.3       ATGGCAGAGGAGAGCAGCTGTACCAGGGATTGCATGTCCTTCAGCGTGCTCAACTGGGAT
                  ************************************************************

HsCD00511099      CAGGTTAGCCGGCTGCATGAGGTCCTCACTGAAGTTGTACCTATCCACGGACGAGGCAAC
NM_017709.3       CAGGTTAGCCGGCTGCATGAGGTCCTCACTGAAGTTGTACCTATCCACGGACGAGGCAAC
                  ************************************************************

HsCD00511099      TTTCCAACCTTGGAGATAACTCTGAAGGACATCGTCCAGACCGTCCGCAGTCGGCTGGAG
NM_017709.3       TTTCCAACCTTGGAGATAACTCTGAAGGACATCGTCCAGACCGTCCGCAGTCGGCTGGAG
                  ************************************************************

HsCD00511099      GAGGCAGGCATCAAAGTGCATGACGTCCGGCTGAATGGCTCCGCAGCTGGCCACGTTTTG
NM_017709.3       GAGGCAGGCATCAAAGTGCACGACGTCCGGCTGAATGGCTCCGCAGCTGGCCACGTTTTG
                  ******************** ***************************************

HsCD00511099      GTCAAAGACAATGGCTTGGGCTGCAAAGACCTGGACCTAATCTTCCATGTGGCTCTTCCA
NM_017709.3       GTCAAAGACAATGGCTTGGGCTGCAAAGACCTGGACCTAATCTTCCATGTGGCTCTTCCA
                  ************************************************************

HsCD00511099      ACAGAGGCAGAATTTCAGCTGGTTAGAGATGTGGTTCTGTGTTCCCTTCTGAACTTCCTG
NM_017709.3       ACAGAGGCAGAATTTCAGCTGGTTAGAGATGTGGTTCTGTGTTCCCTTCTGAACTTCCTG
                  ************************************************************

HsCD00511099      CCAGAGGGTGTGAACAAGCTCAAAATCAGTCCAGTCACTCTGAAGGAGGCATATGTGCAG
NM_017709.3       CCAGAGGGTGTGAACAAGCTCAAAATCAGTCCAGTCACTCTGAAGGAGGCATATGTGCAG
                  ************************************************************

HsCD00511099      AAGCTAGTGAAGGTTTGCACGGACACTGACCGCTGGAGCCTGATCTCCCTCTCCAACAAG
NM_017709.3       AAGCTAGTGAAGGTTTGCACGGACACTGACCGCTGGAGCCTGATCTCCCTCTCCAACAAG
                  ************************************************************

HsCD00511099      AACGGGAAGAACGTGGAGCTGAAGTTTGTCGACTCCATTCGGCGTCAGTTTGAGTTCAGT
NM_017709.3       AACGGGAAGAACGTGGAGCTGAAGTTTGTCGACTCCATTCGGCGTCAGTTTGAGTTCAGT
                  ************************************************************

HsCD00511099      GTGGACTCTTTCCAAATCATCCTGGATTCTTTGCTTTTCTTCTATGACTGTTCCAATAAT
NM_017709.3       GTGGACTCTTTCCAAATCATCCTGGATTCTTTGCTTTTCTTCTATGACTGTTCCAATAAT
                  ************************************************************

HsCD00511099      CCCATCTCTGAGCACTTCCACCCCACCGTGATTGGGGAGAGCATGTACGGGGACTTTGAG
NM_017709.3       CCCATCTCTGAGCACTTCCACCCCACCGTGATTGGGGAGAGCATGTACGGGGACTTTGAG
                  ************************************************************

HsCD00511099      GAAGCTTTTGACCATCTGCAGAACAGACTGATCGCCACCAAGAACCCAGAAGAAATCAGA
NM_017709.3       GAAGCTTTTGACCATCTGCAGAACAGACTGATCGCCACCAAGAACCCAGAAGAAATCAGA
                  ************************************************************

HsCD00511099      GGCGGGGGACTTCTCAAGTACAGCAACCTTCTTGTGCGGGACTTCAGGCCCACAGACCAG
NM_017709.3       GGCGGGGGACTTCTCAAGTACAGCAACCTTCTTGTGCGGGACTTCAGGCCCACAGACCAG
                  ************************************************************

HsCD00511099      GAAGAAATCAAAACTCTAGAGCGCTACATGTGCTCCAGGTTCTTCATCGACTTCCCGGAC
NM_017709.3       GAAGAAATCAAAACTCTAGAGCGCTACATGTGCTCCAGGTTCTTCATCGACTTCCCGGAC
                  ************************************************************

HsCD00511099      ATCCTTGAACAGCAGAGGAAGTTGGAGACTTACCTTCAAAACCACTTCGCTGAAGAAGAG
NM_017709.3       ATCCTTGAACAGCAGAGGAAGTTGGAGACTTACCTTCAAAACCACTTCGCTGAAGAAGAG
                  ************************************************************

HsCD00511099      AGAAGCAAGTACGACTACCTCATGATCCTTCGCAGGGTGGTGAACGAGAGCACCGTGTGT
NM_017709.3       AGAAGCAAGTACGACTACCTCATGATCCTTCGCAGGGTGGTGAACGAGAGCACCGTGTGT
                  ************************************************************

HsCD00511099      CTCATGGGGCATGAACGCAGGCAGACTCTGAACCTCATCTCCCTCCTGGCCTTGCGTGTG
NM_017709.3       CTCATGGGGCATGAACGCAGGCAGACTCTGAACCTCATCTCCCTCCTGGCCTTGCGTGTG
                  ************************************************************

HsCD00511099      CTGGCGGAACAAAACATCATCCCCAGTGCCACCAACGTCACCTGTTACTACCAGCCGGCC
NM_017709.3       CTGGCGGAACAAAACATCATCCCCAGTGCCACCAACGTCACCTGTTACTACCAGCCGGCC
                  ************************************************************

HsCD00511099      CCTTACGTCAGTGATGGCAACTTCAGCAACTACTACGTTGCCCATCCTCCAGTCACCTAC
NM_017709.3       CCTTACGTCAGTGATGGCAACTTCAGCAACTACTACGTTGCCCATCCTCCAGTCACCTAC
                  ************************************************************

HsCD00511099      AGCCAGCCTTACCCTACCTGGCTGCCCTGTAAC---
NM_017709.3       AGCCAGCCTTACCCTACCTGGCTGCCCTGTAACTAA
                  *********************************   

Protein

SMART domain : DUF1693 : Domain of unknown function (DUF1693)
HPRD : 13312

Clone

Cloning Information : 668599
Original Clone ID : ccsbBroadEn_08421

Organism


TAX_ID : 9606
Species Specific ID: 54855

Genome

Chromosome : 1
Map Location : 1p12
Ensembl : ENSG00000183508

Reagents

Labome : FAM46C-antibody