DNASU Plasmid | SNAPC1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: SNAPC1

Gene Name: small nuclear RNA activating complex, polypeptide 1, 43kDa

Original Clone ID:

ccsbBroadEn_01560

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC014984.1,BC019038.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00511631

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1105nts

Open reading frame: 1 to 1105

Reference Sequence Alignment

ATGGGGACTCCTCCCGGCCTGCAGACCGACTGCGAGGCGCTGCTCAGCCGCTTCCAGGAG
ACGGACAGTGTACGCTTCGAGGACTTCACGGAGCTCTGGAGAAACATGAAGTTCGGGACT
ATCTTCTGTGGCAGAATGAGAAATTTAGAAAAGAACATGTTTACAAAAGAAGCTTTAGCT
TTGGCTTGGCGATATTTTTTACCTCCATACACCTTCCAGATCAGAGTTGGTGCTTTGTAT
CTGCTATATGGATTATATAATACCCAACTGTGTCAACCAAAACAAAAGATCAGAGTTGCC
CTGAAGGATTGGGATGAAGTTTTAAAATTTCAGCAAGATTTAGTAAATGCACAGCATTTT
GATGCAGCTTATATTTTTAGGAAGCTACGACTAGACAGAGCATTTCACTTTACAGCAATG
CCCAAATTGCTGTCATATAGGATGAAGAAAAAAATTCACCGAGCTGAAGTTACAGAAGAA
TTTAAGGACCCAAGTGATCGTGTGATGAAACTTATCACTTCTGATGTATTAGAGGAAATG
CTGAATGTTCATGATCATTATCAGAACATGAAACATGTAATTTCAGTTGATAAGTCCAAG
CCAGATAAAGCCCTCAGCTTGATAAAGGATGATTTTTTTGACAATATTAAGAACATAGTT
TTGGAGCATCAGCAGTGGCACAAAGACAGAAAGAATCCATCCTTAAAGTCAAAAACTAAT
GATGGAGAAGAAAAAATGGAAGGAAATTCACAAGAAACGGAGAGATGTGAAAGGGCAGAA
TCATTAGCGAAAATAAAATCAAAGGCCTTTTCAGTTGTCATACAGGCATCCAAATCAAGA
AGGCATCGTCAAGTCAAACTCGACTCTTCTGACTCTGATTCTGCATCTGGTCAAGGGCAA
GTCAAAGCAACTAGGAAAAAAGAGAAGAAAGAAAGATTGAAACCAGCAGGAAGGAAGATG
TCTCTCAGAAACAAAGGCAATGTGCAGAATATACACAAGGAAGATAAACCTTTAAGTCTG
AGTATGCCTGTAATTACAGAAGAAGAAGAGAATGAAAGTTTGAGTGGAACAGAGTTCACT
GCATCCAAGAAGAGGAGAAAACAC

Protein sequence predicted based on nucleotide sequence

MGTPPGLQTDCEALLSRFQETDSVRFEDFTELWRNMKFGTIFCGRMRNLEKNMFTKEALA
LAWRYFLPPYTFQIRVGALYLLYGLYNTQLCQPKQKIRVALKDWDEVLKFQQDLVNAQHF
DAAYIFRKLRLDRAFHFTAMPKLLSYRMKKKIHRAEVTEEFKDPSDRVMKLITSDVLEEM
LNVHDHYQNMKHVISVDKSKPDKALSLIKDDFFDNIKNIVLEHQQWHKDRKNPSLKSKTN
DGEEKMEGNSQETERCERAESLAKIKSKAFSVVIQASKSRRHRQVKLDSSDSDSASGQGQ
VKATRKKEKKERLKPAGRKMSLRNKGNVQNIHKEDKPLSLSMPVITEEEENESLSGTEFT
ASKKRRKH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

SNAPC1

Symbol Nomenclature :

SNAPC1

SYNONYM :

PTFgamma; SNAP43

Designation :

Full Nomenclature :

small nuclear RNA activating complex, polypeptide 1, 43kDa

GENEID :

6617

GenBank Accession :

DQ895926

HGNC :

11134

MIM :

600591

Vega :

OTTHUMG00000140343

Target GenBank:

DQ895926

Reference Sequence Alignment

  

HsCD00511631      ATGGGGACTCCTCCCGGCCTGCAGACCGACTGCGAGGCGCTGCTCAGCCGCTTCCAGGAG
NM_003082.3       ATGGGGACTCCTCCCGGCCTGCAGACCGACTGCGAGGCGCTGCTCAGCCGCTTCCAGGAG
                  ************************************************************

HsCD00511631      ACGGACAGTGTACGCTTCGAGGACTTCACGGAGCTCTGGAGAAACATGAAGTTCGGGACT
NM_003082.3       ACGGACAGTGTACGCTTCGAGGACTTCACGGAGCTCTGGAGAAACATGAAGTTCGGGACT
                  ************************************************************

HsCD00511631      ATCTTCTGTGGCAGAATGAGAAATTTAGAAAAGAACATGTTTACAAAAGAAGCTTTAGCT
NM_003082.3       ATCTTCTGTGGCAGAATGAGAAATTTAGAAAAGAACATGTTTACAAAAGAAGCTTTAGCT
                  ************************************************************

HsCD00511631      TTGGCTTGGCGATATTTTTTACCTCCATACACCTTCCAGATCAGAGTTGGTGCTTTGTAT
NM_003082.3       TTGGCTTGGCGATATTTTTTACCTCCATACACCTTCCAGATCAGAGTTGGTGCTTTGTAT
                  ************************************************************

HsCD00511631      CTGCTATATGGATTATATAATACCCAACTGTGTCAACCAAAACAAAAGATCAGAGTTGCC
NM_003082.3       CTGCTATATGGATTATATAATACCCAACTGTGTCAACCAAAACAAAAGATCAGAGTTGCC
                  ************************************************************

HsCD00511631      CTGAAGGATTGGGATGAAGTTTTAAAATTTCAGCAAGATTTAGTAAATGCACAGCATTTT
NM_003082.3       CTGAAGGATTGGGATGAAGTTTTAAAATTTCAGCAAGATTTAGTAAATGCACAGCATTTT
                  ************************************************************

HsCD00511631      GATGCAGCTTATATTTTTAGGAAGCTACGACTAGACAGAGCATTTCACTTTACAGCAATG
NM_003082.3       GATGCAGCTTATATTTTTAGGAAGCTACGACTAGACAGAGCATTTCACTTTACAGCAATG
                  ************************************************************

HsCD00511631      CCCAAATTGCTGTCATATAGGATGAAGAAAAAAATTCACCGAGCTGAAGTTACAGAAGAA
NM_003082.3       CCCAAATTGCTGTCATATAGGATGAAGAAAAAAATTCACCGAGCTGAAGTTACAGAAGAA
                  ************************************************************

HsCD00511631      TTTAAGGACCCAAGTGATCGTGTGATGAAACTTATCACTTCTGATGTATTAGAGGAAATG
NM_003082.3       TTTAAGGACCCAAGTGATCGTGTGATGAAACTTATCACTTCTGATGTATTAGAGGAAATG
                  ************************************************************

HsCD00511631      CTGAATGTTCATGATCATTATCAGAACATGAAACATGTAATTTCAGTTGATAAGTCCAAG
NM_003082.3       CTGAATGTTCATGATCATTATCAGAACATGAAACATGTAATTTCAGTTGATAAGTCCAAG
                  ************************************************************

HsCD00511631      CCAGATAAAGCCCTCAGCTTGATAAAGGATGATTTTTTTGACAATATTAAGAACATAGTT
NM_003082.3       CCAGATAAAGCCCTCAGCTTGATAAAGGATGATTTTTTTGACAATATTAAGAACATAGTT
                  ************************************************************

HsCD00511631      TTGGAGCATCAGCAGTGGCACAAAGACAGAAAGAATCCATCCTTAAAGTCAAAAACTAAT
NM_003082.3       TTGGAGCATCAGCAGTGGCACAAAGACAGAAAGAATCCATCCTTAAAGTCAAAAACTAAT
                  ************************************************************

HsCD00511631      GATGGAGAAGAAAAAATGGAAGGAAATTCACAAGAAACGGAGAGATGTGAAAGGGCAGAA
NM_003082.3       GATGGAGAAGAAAAAATGGAAGGAAATTCACAAGAAACGGAGAGATGTGAAAGGGCAGAA
                  ************************************************************

HsCD00511631      TCATTAGCGAAAATAAAATCAAAGGCCTTTTCAGTTGTCATACAGGCATCCAAATCAAGA
NM_003082.3       TCATTAGCGAAAATAAAATCAAAGGCCTTTTCAGTTGTCATACAGGCATCCAAATCAAGA
                  ************************************************************

HsCD00511631      AGGCATCGTCAAGTCAAACTCGACTCTTCTGACTCTGATTCTGCATCTGGTCAAGGGCAA
NM_003082.3       AGGCATCGTCAAGTCAAACTCGACTCTTCTGACTCTGATTCTGCATCTGGTCAAGGGCAA
                  ************************************************************

HsCD00511631      GTCAAAGCAACTAGGAAAAAAGAGAAGAAAGAAAGATTGAAACCAGCAGGAAGGAAGATG
NM_003082.3       GTCAAAGCAACTAGGAAAAAAGAGAAGAAAGAAAGATTGAAACCAGCAGGAAGGAAGATG
                  ************************************************************

HsCD00511631      TCTCTCAGAAACAAAGGCAATGTGCAGAATATACACAAGGAAGATAAACCTTTAAGTCTG
NM_003082.3       TCTCTCAGAAACAAAGGCAATGTGCAGAATATACACAAGGAAGATAAACCTTTAAGTCTG
                  ************************************************************

HsCD00511631      AGTATGCCTGTAATTACAGAAGAAGAAGAGAATGAAAGTTTGAGTGGAACAGAGTTCACT
NM_003082.3       AGTATGCCTGTAATTACAGAAGAAGAAGAGAATGAAAGTTTGAGTGGAACAGAGTTCACT
                  ************************************************************

HsCD00511631      GCATCCAAGAAGAGGAGAAAACAC---
NM_003082.3       GCATCCAAGAAGAGGAGAAAACACTGA
                  ************************

Pathways

Reactome :

Gene Expression

Reactome :

RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription

Reactome :

RNA Polymerase III Abortive And Retractive Initiation

Reactome :

RNA Polymerase III Transcription

Reactome :

RNA Polymerase III Transcription Initiation

Reactome :

RNA Polymerase III Transcription Initiation From Type 3 Promoter

Reactome :

Transcription

Protein

HPRD :

08998

Clone

Cloning Information :

605218

Original Clone ID :

ccsbBroadEn_01560

Organism

Species Specific ID:

6617

Genome

Chromosome :

Map Location :

14q22

Ensembl :

ENSG00000023608

Reagents

Labome :

SNAPC1-antibody