DNASU Plasmid | NDUFV3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NDUFV3

Gene Name: NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa

Original Clone ID:

ccsbBroadEn_06626

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC021217.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512266

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1420nts

Open reading frame: 1 to 1420

Reference Sequence Alignment

ATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTCCAG
GAAGCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGGAAG
AGTGAAAAGGGTCAGCCACAGAATTCCAAGAAGCAAAGTCCACCAAAAAATGTAGTGGAA
CCAAAGGAGAGGGGCAAGCTCCTAGCCACCCAGACAGCAGCTGAATTGTCTAAAAACTTA
TCTTCACCCAGTTCTTACCCGCCAGCTGTGAATAAGGGCAGGAAGGTAGCTAGTCCCAGT
CCCAGTGGCAGCGTGCTATTCACAGATGAAGGGGTTCCGAAATTTTTGTCAAGAAAGACT
TTGGTAGAGTTTCCACAGAAAGTTCTGTCTCCATTCAGAAAACAGGGCTCTGATTCAGAA
GCTCGTCAGGTGGGTCGGAAAGTGACGTCGCCTTCGTCTTCATCCTCGTCCAGCTCCTCT
GATTCTGAATCTGATGATGAGGCTGACGTTTCAGAGGTCACTCCTCGAGTGGTGAGCAAA
GGCAGAGGGGGGCTTCGAAAACCAGAGGCCTCTCATTCCTTTGAAAACAGAGCCCCCCGA
GTTACAGTATCAGCAAAAGAGAAAACCTTGCTGCAGAAGCCGCATGTGGACATTACTGAT
CCAGAGAAGCCCCACCAGCCAAAGAAGAAAGGGTCCCCTGCTAAGCCATCAGAAGGCAGG
GAAAATGCGAGACCAAAAACCACAATGCCCAGATCTCAAGTAGATGAAGAGTTTTTGAAG
CAAAGTTTAAAGGAAAAACAATTGCAGAAAACATTTAGATTAAATGAAATAGATAAAGAA
AGCCAAAAGCCATTTGAAGTTAAAGGACCCTTACCTGTCCACACAAAATCAGGGTTGTCT
GCGCCACCGAAGGGCAGCCCAGCGCCTGCTGTGTTGGCAGAAGAGGCCAGAGCAGAGGGG
CAGCTGCAAGCCAGTCCTCCTGGGGCGGCAGAGGGGCATCTGGAAAAACCCGTGCCAGAG
CCCCAGCGCAAGGCGGCCCCTCCCCTGCCCAGAAAGGAAACCTCAGGGACGCAGGGAATA
GAAGGCCACCTGAAGGGTGGACAGGCAATCGTGGAAGATCAGATACCACCAAGCAATTTG
GAGACAGTTCCTGTTGAGAATAACCACGGTTTCCATGAAAAGACAGCAGCGCTGAAGCTT
GAGGCCGAGGGCGAGGCCATGGAAGATGCAGCCGCGCCAGGGAACGACCGAGGCGGCACA
CAGGAGCCAGCCCCAGTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAG
CATCATGACTACAGCACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGG
ATGCCTCAGCCCTCCTCAGGCCGGGAGTCACCTCGACAC

Protein sequence predicted based on nucleotide sequence

MAAPCLLRQGRAGALKTMLQEAQVFRGLASTVSLSAESGKSEKGQPQNSKKQSPPKNVVE
PKERGKLLATQTAAELSKNLSSPSSYPPAVNKGRKVASPSPSGSVLFTDEGVPKFLSRKT
LVEFPQKVLSPFRKQGSDSEARQVGRKVTSPSSSSSSSSSDSESDDEADVSEVTPRVVSK
GRGGLRKPEASHSFENRAPRVTVSAKEKTLLQKPHVDITDPEKPHQPKKKGSPAKPSEGR
ENARPKTTMPRSQVDEEFLKQSLKEKQLQKTFRLNEIDKESQKPFEVKGPLPVHTKSGLS
APPKGSPAPAVLAEEARAEGQLQASPPGAAEGHLEKPVPEPQRKAAPPLPRKETSGTQGI
EGHLKGGQAIVEDQIPPSNLETVPVENNHGFHEKTAALKLEAEGEAMEDAAAPGNDRGGT
QEPAPVPAEPFDNTTYKNLQHHDYSTYTFLDLNLELSKFRMPQPSSGRESPRH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NDUFV3

Symbol Nomenclature :

NDUFV3

SYNONYM :

CI-10k; CI-9KD

Designation :

NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial|NADH-ubiquinone oxidoreductase 9 kD subunit|NADH-ubiquinone oxidoreductase 9 kDa subunit|NADH-ubiquinone oxidoreductase flavoprotein 3, 10kD|complex I 10kDa subunit|complex I, mitochondrial respiratory chain, 10-kD subunit|complex I-9kD|mitochondrial NADH oxidoreductase-like protein|renal carcinoma antigen NY-REN-4

Full Nomenclature :

NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa

GENEID :

4731

GenBank Accession :

DQ895967

HGNC :

7719

MIM :

602184

Target GenBank:

DQ895967

Reference Sequence Alignment

  

HsCD00512266      ATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTCCAG
NM_021075.3       ATGGCTGCCCCGTGTTTGCTGCGGCAAGGACGAGCCGGGGCGCTGAAGACTATGCTCCAG
                  ************************************************************

HsCD00512266      GAAGCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGGAAG
NM_021075.3       GAAGCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATCAGGGAAG
                  ************************************************************

HsCD00512266      AGTGAAAAGGGTCAGCCACAGAATTCCAAGAAGCAAAGTCCACCAAAAAATGTAGTGGAA
NM_021075.3       AGTGAAAAGGGTCAGCCACAGAATTCCAAGAAGCAAAGTCCACCAAAAAATGTAGTGGAA
                  ************************************************************

HsCD00512266      CCAAAGGAGAGGGGCAAGCTCCTAGCCACCCAGACAGCAGCTGAATTGTCTAAAAACTTA
NM_021075.3       CCAAAGGAGAGGGGCAAGCTCCTAGCCACCCAGACAGCAGCTGAATTGTCTAAAAACTTA
                  ************************************************************

HsCD00512266      TCTTCACCCAGTTCTTACCCGCCAGCTGTGAATAAGGGCAGGAAGGTAGCTAGTCCCAGT
NM_021075.3       TCTTCACCCAGTTCTTACCCGCCAGCTGTGAATAAGGGCAGGAAGGTAGCTAGTCCCAGT
                  ************************************************************

HsCD00512266      CCCAGTGGCAGCGTGCTATTCACAGATGAAGGGGTTCCGAAATTTTTGTCAAGAAAGACT
NM_021075.3       CCCAGTGGCAGCGTGCTATTCACAGATGAAGGGGTTCCGAAATTTTTGTCAAGAAAGACT
                  ************************************************************

HsCD00512266      TTGGTAGAGTTTCCACAGAAAGTTCTGTCTCCATTCAGAAAACAGGGCTCTGATTCAGAA
NM_021075.3       TTGGTAGAGTTTCCACAGAAAGTTCTGTCTCCATTCAGAAAACAGGGCTCTGATTCAGAA
                  ************************************************************

HsCD00512266      GCTCGTCAGGTGGGTCGGAAAGTGACGTCGCCTTCGTCTTCATCCTCGTCCAGCTCCTCT
NM_021075.3       GCTCGTCAGGTGGGTCGGAAAGTGACGTCGCCTTCGTCTTCATCCTCTTCCAGCTCCTCT
                  *********************************************** ************

HsCD00512266      GATTCTGAATCTGATGATGAGGCTGACGTTTCAGAGGTCACTCCTCGAGTGGTGAGCAAA
NM_021075.3       GATTCTGAATCTGATGATGAGGCTGACGTTTCAGAGGTCACTCCTCGAGTGGTGAGCAAA
                  ************************************************************

HsCD00512266      GGCAGAGGGGGGCTTCGAAAACCAGAGGCCTCTCATTCCTTTGAAAACAGAGCCCCCCGA
NM_021075.3       GGCAGAGGGGGGCTTCGAAAACCAGAGGCCTCTCATTCCTTTGAAAACAGAGCCCCCCGA
                  ************************************************************

HsCD00512266      GTTACAGTATCAGCAAAAGAGAAAACCTTGCTGCAGAAGCCGCATGTGGACATTACTGAT
NM_021075.3       GTTACAGTATCAGCAAAAGAGAAAACCTTGCTGCAGAAGCCGCATGTGGACATTACTGAT
                  ************************************************************

HsCD00512266      CCAGAGAAGCCCCACCAGCCAAAGAAGAAAGGGTCCCCTGCTAAGCCATCAGAAGGCAGG
NM_021075.3       CCAGAGAAGCCCCACCAGCCAAAGAAGAAAGGGTCCCCTGCTAAGCCATCAGAAGGCAGG
                  ************************************************************

HsCD00512266      GAAAATGCGAGACCAAAAACCACAATGCCCAGATCTCAAGTAGATGAAGAGTTTTTGAAG
NM_021075.3       GAAAATGCGAGACCAAAAACCACAATGCCCAGATCTCAAGTAGATGAAGAGTTTTTGAAG
                  ************************************************************

HsCD00512266      CAAAGTTTAAAGGAAAAACAATTGCAGAAAACATTTAGATTAAATGAAATAGATAAAGAA
NM_021075.3       CAAAGTTTAAAGGAAAAACAATTGCAGAAAACATTTAGATTAAATGAAATAGATAAAGAA
                  ************************************************************

HsCD00512266      AGCCAAAAGCCATTTGAAGTTAAAGGACCCTTACCTGTCCACACAAAATCAGGGTTGTCT
NM_021075.3       AGCCAAAAGCCATTTGAAGTTAAAGGACCCTTACCTGTCCACACAAAATCAGGGTTGTCT
                  ************************************************************

HsCD00512266      GCGCCACCGAAGGGCAGCCCAGCGCCTGCTGTGTTGGCAGAAGAGGCCAGAGCAGAGGGG
NM_021075.3       GCGCCACCGAAGGGCAGCCCAGCGCCTGCTGTGTTGGCAGAAGAGGCCAGAGCAGAGGGG
                  ************************************************************

HsCD00512266      CAGCTGCAAGCCAGTCCTCCTGGGGCGGCAGAGGGGCATCTGGAAAAACCCGTGCCAGAG
NM_021075.3       CAGCTGCAAGCCAGTCCTCCTGGGGCGGCAGAGGGGCATCTGGAAAAACCCGTGCCAGAG
                  ************************************************************

HsCD00512266      CCCCAGCGCAAGGCGGCCCCTCCCCTGCCCAGAAAGGAAACCTCAGGGACGCAGGGAATA
NM_021075.3       CCCCAGCGCAAGGCGGCCCCTCCCCTGCCCAGAAAGGAAACCTCAGGGACGCAGGGAATA
                  ************************************************************

HsCD00512266      GAAGGCCACCTGAAGGGTGGACAGGCAATCGTGGAAGATCAGATACCACCAAGCAATTTG
NM_021075.3       GAAGGCCACCTGAAGGGTGGACAGGCAATCGTGGAAGATCAGATACCACCAAGCAATTTG
                  ************************************************************

HsCD00512266      GAGACAGTTCCTGTTGAGAATAACCACGGTTTCCATGAAAAGACAGCAGCGCTGAAGCTT
NM_021075.3       GAGACAGTTCCTGTTGAGAATAACCACGGTTTCCATGAAAAGACAGCAGCGCTGAAGCTT
                  ************************************************************

HsCD00512266      GAGGCCGAGGGCGAGGCCATGGAAGATGCAGCCGCGCCAGGGAACGACCGAGGCGGCACA
NM_021075.3       GAGGCCGAGGGCGAGGCCATGGAAGATGCAGCCGCGCCAGGGGACGACCGAGGCGGCACA
                  ******************************************.*****************

HsCD00512266      CAGGAGCCAGCCCCAGTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAG
NM_021075.3       CAGGAGCCAGCCCCAGTGCCTGCTGAGCCGTTTGACAACACTACCTACAAGAACCTGCAG
                  ************************************************************

HsCD00512266      CATCATGACTACAGCACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGG
NM_021075.3       CATCATGACTACAGCACGTACACCTTCTTAGACCTCAACCTCGAACTCTCAAAATTCAGG
                  ************************************************************

HsCD00512266      ATGCCTCAGCCCTCCTCAGGCCGGGAGTCACCTCGACAC---
NM_021075.3       ATGCCTCAGCCCTCCTCAGGCCGGGAGTCACCTCGACACTGA
                  ***************************************

Pathways

NCI :

FOXA1 transcription factor network

NCI :

Validated nuclear estrogen receptor alpha network

Reactome :

Metabolism

Reactome :

Respiratory electron transport

Reactome :

Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.

Reactome :

The citric acid (TCA) cycle and respiratory electron transport

WikiPathway :

Electron Transport Chain

WikiPathway :

Oxidative phosphorylation

Protein

HPRD :

03716

Clone

Cloning Information :

604445

Original Clone ID :

ccsbBroadEn_06626

Organism

Species Specific ID:

4731

Genome

Chromosome :

Map Location :

21q22.3

Reagents

Labome :

NDUFV3-antibody