DNASU Plasmid | DCAF4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DCAF4

Gene Name: DDB1 and CUL4 associated factor 4

Original Clone ID:

ccsbBroadEn_07988

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC018979.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512524

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1486nts

Open reading frame: 1 to 1486

Reference Sequence Alignment

ATGAATAAAAGTCGCTGGCAGAGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAAC
CCTTGGTTCAGACTCCGTGATTCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCT
CACGATTCCGGCCACGGTGATGACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACC
TCCTCTGTGCCAGAGCTACCTGGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGC
TTGCTCCCTGGACATAACAACTGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAG
ATGGAGAGCAAGAGACTGCGGCTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGG
ATGGGATTTAATGCATCTTCCATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACC
AATTACTGCCATTTAGCCCACGAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAG
ATTCGAAGCATGGATCCCTCCGCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGAT
ACCAACAGTGACCGGCTCTTCACAGTGAACGATGTTACAGTTGGAGGCTCCAAGTATGGT
ATCATCAACCTGCAAAGTCTGAAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTC
TACTTCACCAACCGGAAGGTGAATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCC
CACATTCTGCTATGCCTCATGGGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCA
GCATCACTGTTCGTCAATAGTCACCCAGGAATAGACCGGCCTGGCATGCTCTGCAGTTTC
CGGATCCCTGGTGCCTGGTCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTC
AGTACAGGCTTGTCTCGGCGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCC
TTTGGGACCAACAGTGATGTCTTGGCCCAGCAGTTTGCTTTCATGGCTCCTCTGCTGTTT
AATGGCTGCCGCTCTGGGGAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAG
GGATGGAAGGCCACCCGCCTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAA
GATGAGCAATACCTGATGGCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGG
ACCACGAAGTGCGTAAGGCAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTG
CATGTGCACGAGGAAGAAGGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGATAATC
TGGAGCCTCCACGATGCCCGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAG
GCCGACATTCCCAGTGTGGCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGG
CTGCTCATGGCTGTCGGGCAGGACCTTTACTGTTACTCCTACAGC

Protein sequence predicted based on nucleotide sequence

MNKSRWQSRRRHGRRSHQQNPWFRLRDSEDRSDSRAAQPAHDSGHGDDESPSTSSGTAGT
SSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRKKIAR
MGFNASSMLRKSQLGFLNVTNYCHLAHELRLSCMERKKVQIRSMDPSALASDRFNLILAD
TNSDRLFTVNDVTVGGSKYGIINLQSLKTPTLKVFMHENLYFTNRKVNSVCWASLNHLDS
HILLCLMGLAETPGCATLLPASLFVNSHPGIDRPGMLCSFRIPGAWSCAWSLNIQANNCF
STGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFAFMAPLLFNGCRSGEIFAIDLRCGNQGK
GWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNEYAYLPL
HVHEEEGILVAVGQDCYTIIWSLHDARLLRTIPSPYPASKADIPSVAFSSRLGGSRGAPG
LLMAVGQDLYCYSYS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DCAF4

Symbol Nomenclature :

DCAF4

SYNONYM :

WDR21; WDR21A

Designation :

DDB1- and CUL4-associated factor 4|WD repeat domain 21A|WD repeat-containing protein 21A

Full Nomenclature :

DDB1 and CUL4 associated factor 4

GENEID :

26094

GenBank Accession :

HQ448372

HGNC :

20229

Vega :

OTTHUMG00000160542

Target GenBank:

HQ448372

Reference Sequence Alignment

  

HsCD00512524        ---------------------------------------ATGAATAAAAGTCGCTGGCAG
XM_005267524.1      ATGTATGGATTTTTTTGTTTTTCTCTTTTAGGAACAGAAATGAATAAAAGTCGCTGGCAG
NM_001163509.1      ------------------------------------------------------------
NM_015604.3         ---------------------------------------ATGAATAAAAGTCGCTGGCAG
NM_001163508.1      ---------------------------------------ATGAATAAAAGTCGCTGGCAG
                                                                                

HsCD00512524        AGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGAT
XM_005267524.1      AGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGAT
NM_001163509.1      -------------ATGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGAT
NM_015604.3         AGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGAT
NM_001163508.1      AGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGAT
                                 ***********************************************

HsCD00512524        TCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGAT
XM_005267524.1      TCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGAT
NM_001163509.1      TCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGAT
NM_015604.3         TCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGAT
NM_001163508.1      TCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGAT
                    ************************************************************

HsCD00512524        GACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACCTCCTCTGTGCCAGAGCTACCT
XM_005267524.1      GACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACCTCCTCTGTGCCAGAGCTACCT
NM_001163509.1      GACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACCTCCTCTGT-------------
NM_015604.3         GACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACCTCCTCTGTGCCAGAGCTACCT
NM_001163508.1      GACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACCTCCTCTGTGCCAGAGCTACCT
                    ***********************************************             

HsCD00512524        GGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAAC
XM_005267524.1      GGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAAC
NM_001163509.1      -------------------GCCAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAAC
NM_015604.3         GGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAAC
NM_001163508.1      GGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAAC
                                       ...**************************************

HsCD00512524        TGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGG
XM_005267524.1      TGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGG
NM_001163509.1      TGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGG
NM_015604.3         TGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGG
NM_001163508.1      TGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGG
                    ************************************************************

HsCD00512524        CTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCC
XM_005267524.1      CTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCC
NM_001163509.1      CTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCC
NM_015604.3         CTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCC
NM_001163508.1      CTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCC
                    ************************************************************

HsCD00512524        ATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCAC
XM_005267524.1      ATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCAC
NM_001163509.1      ATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCAC
NM_015604.3         ATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCAC
NM_001163508.1      ATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCAC
                    ************************************************************

HsCD00512524        GAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCC
XM_005267524.1      GAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCC
NM_001163509.1      GAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCC
NM_015604.3         GAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCC
NM_001163508.1      GAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCC
                    ************************************************************

HsCD00512524        GCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTC
XM_005267524.1      GCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTC
NM_001163509.1      GCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTC
NM_015604.3         GCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTC
NM_001163508.1      GCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTC
                    ************************************************************

HsCD00512524        ACAGTGAACGATGTTACAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTG
XM_005267524.1      ACAGTGAACGATGTTAAAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTG
NM_001163509.1      ACAGTGAACGATGTTAAAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTG
NM_015604.3         ACAGTGAACGATGTTAAAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTG
NM_001163508.1      ACAGTGAACGATGTTAAAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTG
                    ****************.*******************************************

HsCD00512524        AAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTG
XM_005267524.1      AAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTG
NM_001163509.1      AAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTG
NM_015604.3         AAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTG
NM_001163508.1      AAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTG
                    ************************************************************

HsCD00512524        AATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATG
XM_005267524.1      AATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATG
NM_001163509.1      AATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATG
NM_015604.3         AATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATG
NM_001163508.1      AATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATG
                    ************************************************************

HsCD00512524        GGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGT
XM_005267524.1      GGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGT
NM_001163509.1      GGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGT
NM_015604.3         GGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGT
NM_001163508.1      GGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGT
                    ************************************************************

HsCD00512524        CACCCAG---GAATAGACCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGG
XM_005267524.1      CACCCAGCAGGAATAGACCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGG
NM_001163509.1      CACCCAGCAGGAATAGACCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGG
NM_015604.3         CACCCAG---GAATAGACCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGG
NM_001163508.1      CACCCAGCAGGAATAGACCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGG
                    *******   **************************************************

HsCD00512524        TCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGG
XM_005267524.1      TCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGG
NM_001163509.1      TCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGG
NM_015604.3         TCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGG
NM_001163508.1      TCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGG
                    ************************************************************

HsCD00512524        CGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGAT
XM_005267524.1      CGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGAT
NM_001163509.1      CGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGAT
NM_015604.3         CGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGAT
NM_001163508.1      CGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGAT
                    ************************************************************

HsCD00512524        GTCTTGGCCCAGCAGTTTGCTTTCATGGCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGG
XM_005267524.1      GTCTTGGCCCAGCAGTTTGCTCTCATGGCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGG
NM_001163509.1      GTCTTGGCCCAGCAGTTTGCTCTCATGGCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGG
NM_015604.3         GTCTTGGCCCAGCAGTTTGCTCTCATGGCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGG
NM_001163508.1      GTCTTG---------------------GCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGG
                    ******                     *********************************

HsCD00512524        GAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGC
XM_005267524.1      GAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGC
NM_001163509.1      GAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGC
NM_015604.3         GAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGC
NM_001163508.1      GAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGC
                    ************************************************************

HsCD00512524        CTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATG
XM_005267524.1      CTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATG
NM_001163509.1      CTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATG
NM_015604.3         CTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATG
NM_001163508.1      CTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATG
                    ************************************************************

HsCD00512524        GCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGG
XM_005267524.1      GCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGG
NM_001163509.1      GCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGG
NM_015604.3         GCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGG
NM_001163508.1      GCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGG
                    ************************************************************

HsCD00512524        CAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAA
XM_005267524.1      CAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAA
NM_001163509.1      CAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAA
NM_015604.3         CAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAA
NM_001163508.1      CAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAA
                    ************************************************************

HsCD00512524        GGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGATAATCTGGAGCCTCCACGATGCC
XM_005267524.1      GGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGAGAATCTGGAGCCTCCACGATGCC
NM_001163509.1      GGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGAGAATCTGGAGCCTCCACGATGCC
NM_015604.3         GGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGAGAATCTGGAGCCTCCACGATGCC
NM_001163508.1      GGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGAGAATCTGGAGCCTCCACGATGCC
                    ************************************* **********************

HsCD00512524        CGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTG
XM_005267524.1      CGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTG
NM_001163509.1      CGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTG
NM_015604.3         CGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTG
NM_001163508.1      CGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTG
                    ************************************************************

HsCD00512524        GCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGG
XM_005267524.1      GCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGG
NM_001163509.1      GCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGG
NM_015604.3         GCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGG
NM_001163508.1      GCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGG
                    ************************************************************

HsCD00512524        CAGGACCTTTACTGTTACTCCTACAGC---
XM_005267524.1      CAGGACCTTTACTGTTACTCCTACAGCTAA
NM_001163509.1      CAGGACCTTTACTGTTACTCCTACAGCTAA
NM_015604.3         CAGGACCTTTACTGTTACTCCTACAGCTAA
NM_001163508.1      CAGGACCTTTACTGTTACTCCTACAGCTAA
                    ***************************