DNASU Plasmid | WDR8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: WRAP73

Gene Name: WD repeat containing, antisense to TP73

Original Clone ID:

ccsbBroadEn_03140

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC086311.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00512861

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1381nts

Open reading frame: 1 to 1381

Reference Sequence Alignment

ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGC
AAGTACCTGGCTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAG
ATCCTTCAGCTGTACACGTGCCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCG
CTCTTCATCCTGTGCGCCATGTACAAGCGAGGGCTGGTGCAGGTCTGGTCTTTAGAGCAG
CCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGCTGGTGGCCTCGTGCTGGAGC
CCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAACCGTCTGGTCC
TTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC
TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTG
AGCATCTTCGTCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGAT
CTCACAGGGATTGAGTGGGCCCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTG
GAGTACAAGATTCTGCTGTACTCATTGGATGGCCGGTTGTTGTCCACGTACAGCGCTTAC
GAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCAGCAGTCAGTTCCTGGCAGTT
GGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAATGATCACGGAG
TTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG
AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCT
CTCCCGAGCTCAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTG
AAACCTGTTACCGACAGAGCAAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCT
GACAGCTACTTCCTGGCGACAAGGAACGACAACATTCCCAATGCCGTCTGGGTCTGGGAC
ATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGTCCCCAGTGCGCGCATTTCAG
TGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCTCTACCTGTGG
TCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT
CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTC
TGCTTCCTGGAGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG

Protein sequence predicted based on nucleotide sequence

MNFSEVFKLSSLLCKFSPDGKYLASCVQYRLVVRDVNTLQILQLYTCLDQIQHIEWSADS
LFILCAMYKRGLVQVWSLEQPEWHCKIDEGSAGLVASCWSPDGRHILNTTEFHLRITVWS
LCTKSVSYIKYPKACLQGITFTRDGRYMALAERRDCKDYVSIFVCSDWQLLRHFDTDTQD
LTGIEWAPNGCVLAVWDTCLEYKILLYSLDGRLLSTYSAYEWSLGIKSVAWSPSSQFLAV
GSYDGKVRILNHVTWKMITEFGHPAAINDPKIVVYKEAEKSPQLGLGCLSFPPPRAGAGP
LPSSESKYEIASVPVSLQTLKPVTDRANPKIGIGMLAFSPDSYFLATRNDNIPNAVWVWD
IQKLRLFAVLEQLSPVRAFQWDPQQPRLAICTGGSRLYLWSPAGCMSVQVPGEGDFAVLS
LCWHLSGDSMALLSKDHFCLCFLETEAVVGTACRQLGGHT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

WRAP73

Symbol Nomenclature :

WRAP73

SYNONYM :

WDR8

Designation :

WD repeat domain 8|WD repeat-containing protein WRAP73|WD repeat-containing protein antisense to TP73

Full Nomenclature :

WD repeat containing, antisense to TP73

GENEID :

49856

Locus Tag :

RP11-46F15.4

GenBank Accession :

HQ447922

HGNC :

12759

MIM :

606040

Vega :

OTTHUMG00000000612

Target GenBank:

HQ447922

Reference Sequence Alignment

  

HsCD00512861      ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGC
NM_017818.3       ATGAACTTCTCCGAGGTATTCAAGCTCTCCAGCTTACTCTGCAAGTTCTCCCCGGACGGC
                  ************************************************************

HsCD00512861      AAGTACCTGGCTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAG
NM_017818.3       AAGTACCTGGCTTCCTGTGTCCAGTACCGGTTAGTGGTCCGGGATGTGAACACCCTTCAG
                  ************************************************************

HsCD00512861      ATCCTTCAGCTGTACACGTGCCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCG
NM_017818.3       ATCCTTCAGCTGTACACGTGCCTAGACCAGATCCAGCACATCGAGTGGTCGGCAGACTCG
                  ************************************************************

HsCD00512861      CTCTTCATCCTGTGCGCCATGTACAAGCGAGGGCTGGTGCAGGTCTGGTCTTTAGAGCAG
NM_017818.3       CTCTTCATCCTGTGCGCCATGTACAAGCGAGGGCTGGTGCAGGTCTGGTCTTTAGAGCAG
                  ************************************************************

HsCD00512861      CCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGCTGGTGGCCTCGTGCTGGAGC
NM_017818.3       CCCGAATGGCACTGCAAAATAGACGAGGGCTCAGCCGGGCTGGTGGCCTCGTGCTGGAGC
                  ************************************************************

HsCD00512861      CCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAACCGTCTGGTCC
NM_017818.3       CCGGACGGGCGCCACATTCTCAACACCACGGAATTCCATCTGCGGATAACCGTCTGGTCC
                  ************************************************************

HsCD00512861      TTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC
NM_017818.3       TTGTGCACAAAATCCGTGTCTTACATCAAATACCCGAAAGCTTGTCTGCAGGGAATCACC
                  ************************************************************

HsCD00512861      TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTG
NM_017818.3       TTCACCAGGGACGGCCGCTACATGGCGCTGGCAGAACGGCGCGACTGCAAAGATTACGTG
                  ************************************************************

HsCD00512861      AGCATCTTCGTCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGAT
NM_017818.3       AGCATCTTCGTCTGCAGTGATTGGCAGCTCCTGCGGCATTTTGATACGGACACCCAGGAT
                  ************************************************************

HsCD00512861      CTCACAGGGATTGAGTGGGCCCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTG
NM_017818.3       CTCACAGGGATTGAGTGGGCCCCAAACGGCTGTGTGCTGGCAGTGTGGGACACCTGCTTG
                  ************************************************************

HsCD00512861      GAGTACAAGATTCTGCTGTACTCATTGGATGGCCGGTTGTTGTCCACGTACAGCGCTTAC
NM_017818.3       GAGTACAAGATTCTGCTGTACTCATTGGATGGCCGGTTGTTGTCCACGTACAGCGCTTAC
                  ************************************************************

HsCD00512861      GAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCAGCAGTCAGTTCCTGGCAGTT
NM_017818.3       GAGTGGTCCCTGGGCATCAAGTCTGTGGCCTGGAGCCCCAGCAGTCAGTTCCTGGCAGTT
                  ************************************************************

HsCD00512861      GGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAATGATCACGGAG
NM_017818.3       GGGAGCTATGATGGAAAGGTGCGCATCCTTAATCACGTGACTTGGAAAATGATCACGGAG
                  ************************************************************

HsCD00512861      TTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG
NM_017818.3       TTTGGGCATCCTGCAGCCATTAATGATCCCAAGATAGTGGTGTATAAGGAGGCCGAGAAG
                  ************************************************************

HsCD00512861      AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCT
NM_017818.3       AGCCCACAGCTGGGACTGGGCTGCCTCTCCTTCCCGCCGCCCCGGGCCGGGGCCGGCCCT
                  ************************************************************

HsCD00512861      CTCCCGAGCTCAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTG
NM_017818.3       CTCCCGAGCTCAGAGAGTAAATATGAGATCGCCTCTGTCCCAGTCTCCTTACAGACACTG
                  ************************************************************

HsCD00512861      AAACCTGTTACCGACAGAGCAAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCT
NM_017818.3       AAACCTGTTACCGACAGAGCAAACCCGAAAATCGGCATAGGAATGCTGGCATTTAGTCCT
                  ************************************************************

HsCD00512861      GACAGCTACTTCCTGGCGACAAGGAACGACAACATTCCCAATGCCGTCTGGGTCTGGGAC
NM_017818.3       GACAGCTACTTCCTGGCGACAAGGAACGACAACATTCCCAATGCCGTCTGGGTCTGGGAC
                  ************************************************************

HsCD00512861      ATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGTCCCCAGTGCGCGCATTTCAG
NM_017818.3       ATTCAGAAGCTGAGGCTGTTCGCGGTGCTCGAGCAGCTGTCCCCAGTGCGCGCATTTCAG
                  ************************************************************

HsCD00512861      TGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCTCTACCTGTGG
NM_017818.3       TGGGACCCGCAGCAGCCGCGGCTGGCCATCTGCACGGGAGGCAGCAGGCTCTACCTGTGG
                  ************************************************************

HsCD00512861      TCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT
NM_017818.3       TCCCCAGCGGGCTGCATGTCGGTGCAGGTGCCTGGGGAAGGCGACTTTGCAGTGCTCTCT
                  ************************************************************

HsCD00512861      CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTC
NM_017818.3       CTGTGCTGGCATTTAAGCGGAGACTCGATGGCCCTCCTCAGCAAGGATCACTTCTGCCTC
                  ************************************************************

HsCD00512861      TGCTTCCTGGAGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG
NM_017818.3       TGCTTCCTGGAGACAGAGGCAGTGGTCGGCACAGCCTGCAGACAGCTGGGCGGCCACACG
                  ************************************************************

HsCD00512861      ---
NM_017818.3       TAG