DNASU Plasmid | DYRK2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DYRK2

Gene Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2

Original Clone ID:

ccsbBroadEn_01929

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC006375.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00513552

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1804nts

Open reading frame: 1 to 1804

Reference Sequence Alignment

ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGG
GACAGCGCCGTTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGC
GGAGTGGGGACTGGCCCGCCCTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCT
GCCGCCGCAGCCCACACGATTGGCGGCAGTAAGCACACAATGAATGATCACCTGCATGTC
GGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGTTTGAGGATAACAGTAACAAG
CGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAACGGGCTTGCCA
GTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA
AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATG
AAGCAATACATGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAA
ATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAAT
GGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTGCCCCACGATCACGTGGCTTAC
AGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGGCCTAC
GATCACAAAGTCCACCAGCACGTGGCCCTAAAGATGGTGCGGAATGAGAAGCGCTTCCAC
CGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC
ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
TTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTC
AGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCAC
AAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAACATTTTGTTAAAGCAGCAGGGT
AGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTTACGAGCATCAGCGTGTCTAC
ACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGCCAGGTATGGC
ATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC
CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATG
CCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGT
TATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGC
CGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGCAGAGAGTGGGGGAACGCGCTG
AAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAACAGTGTTTAGAGTGGGATCCT
GCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAGGCGGTTGCCA
AAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC
ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTG
GCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTT
AGC

Protein sequence predicted based on nucleotide sequence

MLTRKPSAAAPAAYPTGRGGDSAVRQLQASPGLGAGATRSGVGTGPPSPIALPPLRASNA
AAAAHTIGGSKHTMNDHLHVGSHAHGQIQVQQLFEDNSNKRTVLTTQPNGLTTVGKTGLP
VVPERQLDSIHRRQGSSTSLKSMEGMGKVKATPMTPEQAMKQYMQKLTAFEHHEIFSYPE
IYFLGLNAKKRQGMTGGPNNGGYDDDQGSYVQVPHDHVAYRYEVLKVIGKGSFGQVVKAY
DHKVHQHVALKMVRNEKRFHRQAAEEIRILEHLRKQDKDNTMNVIHMLENFTFRNHICMT
FELLSMNLYELIKKNKFQGFSLPLVRKFAHSILQCLDALHKNRIIHCDLKPENILLKQQG
RSGIKVIDFGSSCYEHQRVYTYIQSRFYRAPEVILGARYGMPIDMWSLGCILAELLTGYP
LLPGEDEGDQLACMIELLGMPSQKLLDASKRAKNFVSSKGYPRYCTVTTLSDGSVVLNGG
RSRRGKLRGPPESREWGNALKGCDDPLFLDFLKQCLEWDPAVRMTPGQALRHPWLRRRLP
KPPTGEKTSVKRITESTGAITSISKLPPPSSSASKLRTNLAQMTDANGNIQQRTVLPKLV
S

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DYRK2

Symbol Nomenclature :

DYRK2

Designation :

dual specificity tyrosine-phosphorylation-regulated kinase 2

Full Nomenclature :

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2

GENEID :

8445

GenBank Accession :

JF432719

HGNC :

3093

MIM :

603496

Target GenBank:

JF432719

Reference Sequence Alignment

  

HsCD00513552      ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGG
NM_006482.2       ATGTTAACCAGGAAACCTTCGGCCGCCGCTCCCGCCGCCTACCCGACCGGCCGAGGTGGG
                  ************************************************************

HsCD00513552      GACAGCGCCGTTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGC
NM_006482.2       GACAGCGCCGTTCGTCAGCTTCAGGCTTCCCCGGGGCTCGGTGCAGGGGCCACCCGGAGC
                  ************************************************************

HsCD00513552      GGAGTGGGGACTGGCCCGCCCTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCT
NM_006482.2       GGAGTGGGGACTGGCCCGCCCTCCCCCATCGCCCTGCCGCCTCTCCGGGCCAGCAACGCT
                  ************************************************************

HsCD00513552      GCCGCCGCAGCCCACACGATTGGCGGCAGTAAGCACACAATGAATGATCACCTGCATGTC
NM_006482.2       GCCGCCGCAGCCCACACGATTGGCGGCAGTAAGCACACAATGAATGATCACCTGCATGTC
                  ************************************************************

HsCD00513552      GGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGTTTGAGGATAACAGTAACAAG
NM_006482.2       GGCAGCCACGCTCACGGACAGATCCAGGTTCAACAGTTGTTTGAGGATAACAGTAACAAG
                  ************************************************************

HsCD00513552      CGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAACGGGCTTGCCA
NM_006482.2       CGGACAGTGCTCACGACACAACCAAATGGGCTTACAACAGTGGGCAAAACGGGCTTGCCA
                  ************************************************************

HsCD00513552      GTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA
NM_006482.2       GTGGTGCCAGAGCGGCAGCTGGACAGCATTCATAGACGGCAGGGGAGCTCCACCTCTCTA
                  ************************************************************

HsCD00513552      AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATG
NM_006482.2       AAGTCCATGGAAGGCATGGGGAAGGTGAAAGCCACCCCCATGACACCTGAACAAGCAATG
                  ************************************************************

HsCD00513552      AAGCAATACATGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAA
NM_006482.2       AAGCAATACATGCAAAAACTCACAGCCTTCGAACACCATGAGATTTTCAGCTACCCTGAA
                  ************************************************************

HsCD00513552      ATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAAT
NM_006482.2       ATATATTTCTTGGGTCTAAATGCTAAGAAGCGCCAGGGCATGACAGGTGGGCCCAACAAT
                  ************************************************************

HsCD00513552      GGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTGCCCCACGATCACGTGGCTTAC
NM_006482.2       GGTGGCTATGATGATGACCAGGGATCATATGTGCAGGTGCCCCACGATCACGTGGCTTAC
                  ************************************************************

HsCD00513552      AGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGGCCTAC
NM_006482.2       AGGTATGAGGTCCTCAAGGTCATTGGGAAGGGGAGCTTTGGGCAGGTGGTCAAGGCCTAC
                  ************************************************************

HsCD00513552      GATCACAAAGTCCACCAGCACGTGGCCCTAAAGATGGTGCGGAATGAGAAGCGCTTCCAC
NM_006482.2       GATCACAAAGTCCACCAGCACGTGGCCCTAAAGATGGTGCGGAATGAGAAGCGCTTCCAC
                  ************************************************************

HsCD00513552      CGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC
NM_006482.2       CGGCAAGCAGCGGAGGAGATCCGAATCCTGGAACACCTGCGGAAGCAGGACAAGGATAAC
                  ************************************************************

HsCD00513552      ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
NM_006482.2       ACAATGAATGTCATCCATATGCTGGAGAATTTCACCTTCCGCAACCACATCTGCATGACG
                  ************************************************************

HsCD00513552      TTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTC
NM_006482.2       TTTGAGCTGCTGAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAATTCCAGGGCTTC
                  ************************************************************

HsCD00513552      AGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCAC
NM_006482.2       AGTCTGCCTTTGGTTCGCAAGTTTGCCCACTCGATTCTGCAGTGCTTGGATGCTTTGCAC
                  ************************************************************

HsCD00513552      AAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAACATTTTGTTAAAGCAGCAGGGT
NM_006482.2       AAAAACAGAATAATTCACTGTGACCTTAAGCCCGAGAACATTTTGTTAAAGCAGCAGGGT
                  ************************************************************

HsCD00513552      AGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTTACGAGCATCAGCGTGTCTAC
NM_006482.2       AGAAGCGGTATTAAAGTAATTGATTTTGGCTCCAGTTGTTACGAGCATCAGCGTGTCTAC
                  ************************************************************

HsCD00513552      ACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGCCAGGTATGGC
NM_006482.2       ACGTACATCCAGTCGCGTTTTTACCGGGCTCCAGAAGTGATCCTTGGGGCCAGGTATGGC
                  ************************************************************

HsCD00513552      ATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC
NM_006482.2       ATGCCCATTGATATGTGGAGCCTGGGCTGCATTTTAGCAGAGCTCCTGACGGGTTACCCC
                  ************************************************************

HsCD00513552      CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATG
NM_006482.2       CTCTTGCCTGGGGAAGATGAAGGGGACCAGCTGGCCTGTATGATTGAACTGTTGGGCATG
                  ************************************************************

HsCD00513552      CCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGT
NM_006482.2       CCCTCACAGAAACTGCTGGATGCATCCAAACGAGCCAAAAATTTTGTGAGCTCCAAGGGT
                  ************************************************************

HsCD00513552      TATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGC
NM_006482.2       TATCCCCGTTACTGCACTGTCACGACTCTCTCAGATGGCTCTGTGGTCCTAAACGGAGGC
                  ************************************************************

HsCD00513552      CGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGCAGAGAGTGGGGGAACGCGCTG
NM_006482.2       CGTTCCCGGAGGGGGAAACTGAGGGGCCCACCGGAGAGCAGAGAGTGGGGGAACGCGCTG
                  ************************************************************

HsCD00513552      AAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAACAGTGTTTAGAGTGGGATCCT
NM_006482.2       AAGGGGTGTGATGATCCCCTTTTCCTTGACTTCTTAAAACAGTGTTTAGAGTGGGATCCT
                  ************************************************************

HsCD00513552      GCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAGGCGGTTGCCA
NM_006482.2       GCAGTGCGCATGACCCCAGGCCAGGCTTTGCGGCACCCCTGGCTGAGGAGGCGGTTGCCA
                  ************************************************************

HsCD00513552      AAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC
NM_006482.2       AAGCCTCCCACCGGGGAGAAAACGTCAGTGAAAAGGATAACTGAGAGCACCGGTGCTATC
                  ************************************************************

HsCD00513552      ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTG
NM_006482.2       ACATCTATATCCAAGTTACCTCCACCTTCTAGCTCAGCTTCCAAACTGAGGACTAATTTG
                  ************************************************************

HsCD00513552      GCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTT
NM_006482.2       GCGCAGATGACAGATGCCAATGGGAATATTCAGCAGAGGACAGTGTTGCCAAAACTTGTT
                  ************************************************************

HsCD00513552      AGC---
NM_006482.2       AGCTGA
                  ***