DNASU Plasmid | GRHL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GRHL2

Gene Name: grainyhead-like 2 (Drosophila)

Original Clone ID:

ccsbBroadEn_04157

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC129822.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00514302

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1876nts

Open reading frame: 1 to 1876

Reference Sequence Alignment

ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGAC
CCTCCATTCAATACCCGAAGAGCCTACACCAGTGAGGATGAAGCCTGGAAGTCATACTTG
GAGAATCCCCTGACAGCAGCCACCAAGGCCATGATGAGCATTAATGGTGATGAGGACAGT
GCTGCTGCCCTCGGCCTGCTCTATGACTACTACAAGGTTCCTCGAGACAAGAGGCTGCTG
TCTGTAAGCAAAGCAAGTGACAGCCAAGAAGACCAGGAGAAAAGAAACTGCCTTGGCACC
AGTGAAGCCCAGAGTAATTTGAGTGGAGGAGAAAACCGAGTGCAAGTCCTAAAGACTGTT
CCAGTGAACCTTTCCCTAAATCAAGATCACCTGGAGAATTCCAAGCGGGAACAGTACAGC
ATCAGCTTCCCCGAGAGCTCTGCCATCATCCCGGTGTCGGGAATCACGGTGGTGAAAGCT
GAAGATTTCACACCAGTTTTCATGGCCCCACCTGTGCACTATCCCCGGGGAGATGGGGAA
GAGCAACGAGTGGTTATCTTTGAACAGACTCAGTATGACGTGCCCTCGCTGGCCACCCAC
AGCGCCTATCTCAAAGACGACCAGCGCAGCACTCCGGACAGCACATACAGCGAGAGCTTC
AAGGACGCAGCCACAGAGAAATTTCGGAGTGCTTCAGTTGGGGCTGAGGAGTACATGTAT
GATCAGACATCAAGTGGCACATTTCAGTACACCCTGGAAGCCACCAAATCTCTCCGTCAG
AAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAGTTCTATGCCATAACACTC
AGCGAGACCGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTG
ATGGTGGTCTTCAGTGAAGACAAAAACAGAGATGAACAGCTCAAATACTGGAAATACTGG
CACTCTCGGCAGCATACGGCGAAGCAGAGGGTCCTTGACATTGCCGATTACAAGGAGAGC
TTTAATACGATTGGAAACATTGAAGAGATTGCATATAATGCTGTTTCCTTTACCTGGGAC
GTGAATGAAGAGGCGAAGATTTTCATCACCGTGAATTGCTTGAGCACAGATTTCTCCTCC
CAAAAAGGGGTGAAAGGACTTCCTTTGATGATTCAGATTGACACATACAGTTATAACAAT
CGTAGCAATAAACCCATTCATAGAGCTTATTGCCAGATCAAGGTCTTCTGTGACAAAGGA
GCAGAAAGAAAAATCCGAGATGAAGAGCGGAAGCAGAACAGGAAGAAAGGGAAAGGCCAG
GCCTCCCAAACTCAATGCAACAGCTCCTCTGATGGGAAGTTGGCTGCCATACCTTTACAG
AAGAAGAGTGACATCACCTACTTCAAAACCATGCCTGATCTCCACTCACAGCCAGTTCTC
TTCATACCTGATGTTCACTTTGCAAACCTGCAGAGGACCGGACAGGTGTATTACAACACG
GATGATGAACGAGAAGGTGGCAGTGTCCTTGTTAAACGGATGTTCCGGCCCATGGAAGAG
GAGTTTGGTCCAGTGCCTTCAAAGCAGATGAAAGAAGAAGGGACAAAGCGAGTGCTCTTG
TACGTGAGGAAGGAGACTGACGATGTGTTCGATGCATTGATGTTGAAGTCTCCCACAGTG
AAGGGCCTGATGGAAGCGATATCTGAGAAATATGGGCTGCCCGTGGAGAAGATAGCAAAG
CTTTACAAGAAAAGCAAAAAAGGCATCTTGGTGAACATGGATGACAACATCATCGAGCAC
TACTCGAACGAGGACACCTTCATCCTCAACATGGAGAGCATGGTGGAGGGCTTCAAGGTC
ACGCTCATGGAAATC

Protein sequence predicted based on nucleotide sequence

MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEDS
AAALGLLYDYYKVPRDKRLLSVSKASDSQEDQEKRNCLGTSEAQSNLSGGENRVQVLKTV
PVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGE
EQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMY
DQTSSGTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGDNKCFRHPISKVRSVV
MVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWD
VNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKG
AERKIRDEERKQNRKKGKGQASQTQCNSSSDGKLAAIPLQKKSDITYFKTMPDLHSQPVL
FIPDVHFANLQRTGQVYYNTDDEREGGSVLVKRMFRPMEEEFGPVPSKQMKEEGTKRVLL
YVRKETDDVFDALMLKSPTVKGLMEAISEKYGLPVEKIAKLYKKSKKGILVNMDDNIIEH
YSNEDTFILNMESMVEGFKVTLMEI

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GRHL2

Symbol Nomenclature :

GRHL2

SYNONYM :

BOM; DFNA28; TFCP2L3

Designation :

brother of mammalian grainyhead|grainyhead-like protein 2 homolog|transcription factor CP2-like 3

Full Nomenclature :

grainyhead-like 2 (Drosophila)

GENEID :

79977

HGNC :

2799

MIM :

608576

Vega :

OTTHUMG00000149915

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00514302      ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGAC
NM_024915.3       ATGTCACAAGAGTCGGACAATAATAAAAGACTAGTGGCCTTAGTGCCCATGCCCAGTGAC
                  ************************************************************

HsCD00514302      CCTCCATTCAATACCCGAAGAGCCTACACCAGTGAGGATGAAGCCTGGAAGTCATACTTG
NM_024915.3       CCTCCATTCAATACCCGAAGAGCCTACACCAGTGAGGATGAAGCCTGGAAGTCATACTTG
                  ************************************************************

HsCD00514302      GAGAATCCCCTGACAGCAGCCACCAAGGCCATGATGAGCATTAATGGTGATGAGGACAGT
NM_024915.3       GAGAATCCCCTGACAGCAGCCACCAAGGCCATGATGAGCATTAATGGTGATGAGGACAGT
                  ************************************************************

HsCD00514302      GCTGCTGCCCTCGGCCTGCTCTATGACTACTACAAGGTTCCTCGAGACAAGAGGCTGCTG
NM_024915.3       GCTGCTGCCCTCGGCCTGCTCTATGACTACTACAAGGTTCCTCGAGACAAGAGGCTGCTG
                  ************************************************************

HsCD00514302      TCTGTAAGCAAAGCAAGTGACAGCCAAGAAGACCAGGAGAAAAGAAACTGCCTTGGCACC
NM_024915.3       TCTGTAAGCAAAGCAAGTGACAGCCAAGAAGACCAGGAGAAAAGAAACTGCCTTGGCACC
                  ************************************************************

HsCD00514302      AGTGAAGCCCAGAGTAATTTGAGTGGAGGAGAAAACCGAGTGCAAGTCCTAAAGACTGTT
NM_024915.3       AGTGAAGCCCAGAGTAATTTGAGTGGAGGAGAAAACCGAGTGCAAGTCCTAAAGACTGTT
                  ************************************************************

HsCD00514302      CCAGTGAACCTTTCCCTAAATCAAGATCACCTGGAGAATTCCAAGCGGGAACAGTACAGC
NM_024915.3       CCAGTGAACCTTTCCCTAAATCAAGATCACCTGGAGAATTCCAAGCGGGAACAGTACAGC
                  ************************************************************

HsCD00514302      ATCAGCTTCCCCGAGAGCTCTGCCATCATCCCGGTGTCGGGAATCACGGTGGTGAAAGCT
NM_024915.3       ATCAGCTTCCCCGAGAGCTCTGCCATCATCCCGGTGTCGGGAATCACGGTGGTGAAAGCT
                  ************************************************************

HsCD00514302      GAAGATTTCACACCAGTTTTCATGGCCCCACCTGTGCACTATCCCCGGGGAGATGGGGAA
NM_024915.3       GAAGATTTCACACCAGTTTTCATGGCCCCACCTGTGCACTATCCCCGGGGAGATGGGGAA
                  ************************************************************

HsCD00514302      GAGCAACGAGTGGTTATCTTTGAACAGACTCAGTATGACGTGCCCTCGCTGGCCACCCAC
NM_024915.3       GAGCAACGAGTGGTTATCTTTGAACAGACTCAGTATGACGTGCCCTCGCTGGCCACCCAC
                  ************************************************************

HsCD00514302      AGCGCCTATCTCAAAGACGACCAGCGCAGCACTCCGGACAGCACATACAGCGAGAGCTTC
NM_024915.3       AGCGCCTATCTCAAAGACGACCAGCGCAGCACTCCGGACAGCACATACAGCGAGAGCTTC
                  ************************************************************

HsCD00514302      AAGGACGCAGCCACAGAGAAATTTCGGAGTGCTTCAGTTGGGGCTGAGGAGTACATGTAT
NM_024915.3       AAGGACGCAGCCACAGAGAAATTTCGGAGTGCTTCAGTTGGGGCTGAGGAGTACATGTAT
                  ************************************************************

HsCD00514302      GATCAGACATCAAGTGGCACATTTCAGTACACCCTGGAAGCCACCAAATCTCTCCGTCAG
NM_024915.3       GATCAGACATCAAGTGGCACATTTCAGTACACCCTGGAAGCCACCAAATCTCTCCGTCAG
                  ************************************************************

HsCD00514302      AAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAGTTCTATGCCATAACACTC
NM_024915.3       AAGCAGGGGGAGGGCCCCATGACCTACCTCAACAAAGGACAGTTCTATGCCATAACACTC
                  ************************************************************

HsCD00514302      AGCGAGACCGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTG
NM_024915.3       AGCGAGACCGGAGACAACAAATGCTTCCGACACCCCATCAGCAAAGTCAGGAGTGTGGTG
                  ************************************************************

HsCD00514302      ATGGTGGTCTTCAGTGAAGACAAAAACAGAGATGAACAGCTCAAATACTGGAAATACTGG
NM_024915.3       ATGGTGGTCTTCAGTGAAGACAAAAACAGAGATGAACAGCTCAAATACTGGAAATACTGG
                  ************************************************************

HsCD00514302      CACTCTCGGCAGCATACGGCGAAGCAGAGGGTCCTTGACATTGCCGATTACAAGGAGAGC
NM_024915.3       CACTCTCGGCAGCATACGGCGAAGCAGAGGGTCCTTGACATTGCCGATTACAAGGAGAGC
                  ************************************************************

HsCD00514302      TTTAATACGATTGGAAACATTGAAGAGATTGCATATAATGCTGTTTCCTTTACCTGGGAC
NM_024915.3       TTTAATACGATTGGAAACATTGAAGAGATTGCATATAATGCTGTTTCCTTTACCTGGGAC
                  ************************************************************

HsCD00514302      GTGAATGAAGAGGCGAAGATTTTCATCACCGTGAATTGCTTGAGCACAGATTTCTCCTCC
NM_024915.3       GTGAATGAAGAGGCGAAGATTTTCATCACCGTGAATTGCTTGAGCACAGATTTCTCCTCC
                  ************************************************************

HsCD00514302      CAAAAAGGGGTGAAAGGACTTCCTTTGATGATTCAGATTGACACATACAGTTATAACAAT
NM_024915.3       CAAAAAGGGGTGAAAGGACTTCCTTTGATGATTCAGATTGACACATACAGTTATAACAAT
                  ************************************************************

HsCD00514302      CGTAGCAATAAACCCATTCATAGAGCTTATTGCCAGATCAAGGTCTTCTGTGACAAAGGA
NM_024915.3       CGTAGCAATAAACCCATTCATAGAGCTTATTGCCAGATCAAGGTCTTCTGTGACAAAGGA
                  ************************************************************

HsCD00514302      GCAGAAAGAAAAATCCGAGATGAAGAGCGGAAGCAGAACAGGAAGAAAGGGAAAGGCCAG
NM_024915.3       GCAGAAAGAAAAATCCGAGATGAAGAGCGGAAGCAGAACAGGAAGAAAGGGAAAGGCCAG
                  ************************************************************

HsCD00514302      GCCTCCCAAACTCAATGCAACAGCTCCTCTGATGGGAAGTTGGCTGCCATACCTTTACAG
NM_024915.3       GCCTCCCAAACTCAATGCAACAGCTCCTCTGATGGGAAGTTGGCTGCCATACCTTTACAG
                  ************************************************************

HsCD00514302      AAGAAGAGTGACATCACCTACTTCAAAACCATGCCTGATCTCCACTCACAGCCAGTTCTC
NM_024915.3       AAGAAGAGTGACATCACCTACTTCAAAACCATGCCTGATCTCCACTCACAGCCAGTTCTC
                  ************************************************************

HsCD00514302      TTCATACCTGATGTTCACTTTGCAAACCTGCAGAGGACCGGACAGGTGTATTACAACACG
NM_024915.3       TTCATACCTGATGTTCACTTTGCAAACCTGCAGAGGACCGGACAGGTGTATTACAACACG
                  ************************************************************

HsCD00514302      GATGATGAACGAGAAGGTGGCAGTGTCCTTGTTAAACGGATGTTCCGGCCCATGGAAGAG
NM_024915.3       GATGATGAACGAGAAGGTGGCAGTGTCCTTGTTAAACGGATGTTCCGGCCCATGGAAGAG
                  ************************************************************

HsCD00514302      GAGTTTGGTCCAGTGCCTTCAAAGCAGATGAAAGAAGAAGGGACAAAGCGAGTGCTCTTG
NM_024915.3       GAGTTTGGTCCAGTGCCTTCAAAGCAGATGAAAGAAGAAGGGACAAAGCGAGTGCTCTTG
                  ************************************************************

HsCD00514302      TACGTGAGGAAGGAGACTGACGATGTGTTCGATGCATTGATGTTGAAGTCTCCCACAGTG
NM_024915.3       TACGTGAGGAAGGAGACTGACGATGTGTTCGATGCATTGATGTTGAAGTCTCCCACAGTG
                  ************************************************************

HsCD00514302      AAGGGCCTGATGGAAGCGATATCTGAGAAATATGGGCTGCCCGTGGAGAAGATAGCAAAG
NM_024915.3       AAGGGCCTGATGGAAGCGATATCTGAGAAATATGGGCTGCCCGTGGAGAAGATAGCAAAG
                  ************************************************************

HsCD00514302      CTTTACAAGAAAAGCAAAAAAGGCATCTTGGTGAACATGGATGACAACATCATCGAGCAC
NM_024915.3       CTTTACAAGAAAAGCAAAAAAGGCATCTTGGTGAACATGGATGACAACATCATCGAGCAC
                  ************************************************************

HsCD00514302      TACTCGAACGAGGACACCTTCATCCTCAACATGGAGAGCATGGTGGAGGGCTTCAAGGTC
NM_024915.3       TACTCGAACGAGGACACCTTCATCCTCAACATGGAGAGCATGGTGGAGGGCTTCAAGGTC
                  ************************************************************

HsCD00514302      ACGCTCATGGAAATC---
NM_024915.3       ACGCTCATGGAAATCTAG
                  ***************